检索结果-南通市图书馆

人血浆hdl对高脂饲养家兔肝细胞膜hdl受体活性的影响

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

四川大学学报(医学版) 1999年第4期30卷 370-372页

作者：吴新伟傅明德蓝德宾刘秉文周建均邓萍华西医科大学基础医学院载脂蛋白研究室!成都610041 成都蜀阳制药厂

为进一步探讨ＨＤＬ对抗动脉粥样硬化的作用机理，采用高胆固醇膳食饲养家兔，通过静脉注射人血浆ＨＤＬ制剂，观察ＨＤＬ对高脂饲养家兔血脂、肝脏和胆囊胆汁脂质含量以及肝细胞膜ＨＤＬ受体活性的影响。结果发现：ＨＤＬ制剂虽无降低... 详细信息

为进一步探讨ＨＤＬ对抗动脉粥样硬化的作用机理，采用高胆固醇膳食饲养家兔，通过静脉注射人血浆ＨＤＬ制剂，观察ＨＤＬ对高脂饲养家兔血脂、肝脏和胆囊胆汁脂质含量以及肝细胞膜ＨＤＬ受体活性的影响。结果发现：ＨＤＬ制剂虽无降低高脂饲养家兔血清脂质的作用，但可减少其肝脏脂质沉积，并且具有促进脂质经胆道排泄的作用。高脂饲养家兔的肝细胞膜ＨＤＬ受体具有Ｋｄ值减小，Ｂｍａｘ值增加的趋势；注射ＨＤＬ制剂的家兔，与正常对照比较，其Ｋｄ值虽无明显变化，但Ｂｍａｘ值显著增加（Ｐ＜０．０５），表明注射人血浆ＨＤＬ可使高脂饲养家兔肝细胞膜ＨＤＬ受体呈现以受体数目增加为特征的活性升高。

关键词：动脉粥样硬化 hdl hdl受体肝细胞膜胆固醇逆向转运

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

双亲型吸附剂对hdl的吸附动力学研究

离子交换与吸附 2002年第6期18卷 543-548页

作者：袁毅俞耀庭王深琪南开大学生物活性材料教育部重点实验室南开大学生物活性材料教育部重点实验室天津300071

设计合成了以葡聚糖为载体,同时具有亲水性磺酸基和疏水性胆固醇两类配基的新型吸附剂。通过对高密度脂蛋白 (hdl) 纯溶液中吸附等温线的测试,比较了以Dextran G-75为载体双亲型吸附剂与单一亲水型磺酸基配基、单一疏水型胆固醇配基吸... 详细信息

设计合成了以葡聚糖为载体,同时具有亲水性磺酸基和疏水性胆固醇两类配基的新型吸附剂。通过对高密度脂蛋白 (hdl) 纯溶液中吸附等温线的测试,比较了以Dextran G-75为载体双亲型吸附剂与单一亲水型磺酸基配基、单一疏水型胆固醇配基吸附剂吸附量和亲和吸附系数的关系,对双亲型吸附剂的吸附动力学进行了初步研究。在hdl溶液中,亲水型磺酸基、疏水型胆固醇配基、双亲型吸附剂对hdl的吸附曲线基本符合Langmuir吸附方程。

关键词：双亲性吸附剂 hdl 吸附动力学

hdl通过ANXA1发挥对内皮细胞保护功能

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

中国病理生理杂志 2015年第10期 1808-1809页

作者：潘兵孔金阁赵明明郑乐民北京大学心血管所教育部重点实验室

目的:高密度脂蛋白(hdl)通过抗炎作用发挥重要的心血管保护功能。膜联蛋白A1(ANXA1)最早被发现是一种抗炎因子,能抑制血管损伤的白细胞浸润。本研究探讨了hdl调节内皮细胞中ANXA1对单核细胞黏附的影响及相关机制。方法:采用蛋白质组学... 详细信息

目的:高密度脂蛋白(hdl)通过抗炎作用发挥重要的心血管保护功能。膜联蛋白A1(ANXA1)最早被发现是一种抗炎因子,能抑制血管损伤的白细胞浸润。本研究探讨了hdl调节内皮细胞中ANXA1对单核细胞黏附的影响及相关机制。方法:采用蛋白质组学和质谱鉴定技术研究hdl对人内皮细胞系Hy926蛋白的表达,通过siRNA干扰和抗体阻断分析ANXA1表

关键词： hdl 内皮细胞 ANXA1 保护功能

高脂膳食诱发家兔血清LPO升高及LDL、VLDL和hdl在活体内的氧化修饰

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

华西医科大学学报 1997年第1期28卷 1-5页

作者：江渝刘秉文傅明德华西医科大学基础医学院四川成都

为探讨高脂膳食对家兔血清过氧化脂质（ＬＰＯ）升高和ＬＤＬ、ＶＬＤＬ，特别是ＨＤＬ氧化修饰的影响。正常对照组（ｎ＝８）喂以普通饲料，高脂实验组（ｎ＝８）用高胆固醇饲料（普通饲料加５％猪油，另每只兔加喂０．５ｇ胆固醇）喂... 详细信息

为探讨高脂膳食对家兔血清过氧化脂质（ＬＰＯ）升高和ＬＤＬ、ＶＬＤＬ，特别是ＨＤＬ氧化修饰的影响。正常对照组（ｎ＝８）喂以普通饲料，高脂实验组（ｎ＝８）用高胆固醇饲料（普通饲料加５％猪油，另每只兔加喂０．５ｇ胆固醇）喂养１２周。兔血清ＬＤＬ、ＶＬＤＬ及ＨＤＬ用密度梯度超速离心法分离得到。ＬＤＬ、ＶＬＤＬ及ＨＤＬ的氧化修饰用琼脂糖凝胶电泳、２３４ｎｍ光吸收及ＴＢＡＲＳ荧光检测进行鉴定。结果显示：高脂实验组兔血清的ＴＣ、ＴＧ和ＬＰＯ均显著高于正常对照组（Ｐ＜０．０１），而ＨＤＬ－Ｃ则显著降低（Ｐ＜０．０５）；同时，高脂实验组的ＬＤＬ、ＶＬＤＬ和ＨＤＬ的电泳迁移率增加，其２３４ｎｍ光吸收及ＬＰＯ含量均显著高于正常对照组（Ｐ＜０．０１）。表明高脂膳食在诱发家兔血清ＴＣ、ＴＧ显著增高的同时，不仅可引起血浆ＬＤＬ及ＶＬＤＬ的氧化修饰。

关键词：高脂膳食过氧化脂质 LPO VLDL 高血脂病 hdl

维普期刊数据库

Profiling of human hdl and LDL associated proteins by native 2DE-MS mapping

在线全文

学校读者我要写书评

暂无评论

Profiling of human HDL and LDL associated proteins by native...

The influence of hdl and purified apoprotein E on platelet activation induced by serotonin

2018年中国质谱学术大会（CMSC2018）

作者： Ya Jin Shujie Bu Jin Chen Takashi Manabe Wen Tan Institute of Biomedical and Pharmaceutical Sciences Guangdong University of Technology School of Bioscience and Bioengineering South ChinaUniversity of Technology Faculty of Science Ehime University

Plasma lipoproteins are soluble complexes of proteins and lipids that transport lipids in the circulation. Historically, lipoproteins were categorized and named by density range, thus each class contains a group of molecules varying in size, charges and lipid and protein compositions. Such differences were demonstrated to contribute to their specific physiological functions and pathological roles. However, profiling of the protein content and interactions of lipoproteins has always been analytically challenging and requires methods of high sensitivity and high resolution. In our work, a method employing nondenaturing 2 DE, grid gel-cutting and quantitative LC-MS/MS has been used to analyze the associated proteins in human plasma high-density lipoprotein(hdl) and low-density lipoprotein(LDL) [1,2]. A human plasma sample was separated by nondenaturing 2 DE, the gel areas that were detected by immunochemical staining to contain hdl and LDL were respectively subjected for grid cut. For hdl, the 5 mm × 18 mm area was cut into 90 squares of 1 mm × 1 mm, and each of the gel piece was analyzed in standardized procedures of in-gel digestion and quantitative LC-MS/MS. Totally 154 proteins were detected and the quantity distribution of each was reconstructed as a color-density pattern(a native protein map). The map of apolipoprotein(Apo) A-I showed a wide apparent mass distribution characteristic to hdl and was compared with the maps of the other 153 proteins. Eleven proteins showed maps of wide distribution that overlapped with the map of Apo A-I, and all have been reported to be the components of hdl. Further, seven minor proteins associated with hdl were detected at the gel positions of high Apo A-I quantity. In the analysis of LDL, the 18 mm × 4.8 mm area was cut into 72 squares. A total of 253 proteins were detected and their native maps were reconstructed. Apo B-100 was shown to be the most abundant protein in the grid-cut area, concentrated at p I ca. 5.4–6.1 and appare

关键词： human lipoproteins hdl LDL nondenaturing 2DE LC-MS/MS mapping

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Chinese Medical Journal 1998年第1期111卷 91-91页

作者： Olaf Pfennig, Bing Liu and R.Dierichs Platelet Research Unit Institute of Anatomy University of Münster Germany Institute of Physiology Ruhr-University Bochum Bochum Germany

Elevated plasma levels of high density lipoprotein (hdl) are recognized as having a beneficial influence on the progression of atherosclerosis. As platelets are closely involved in atherosclerogenesis, it is difficult to evaluate if the protective effect of hdl is associated with its ability to affect platelet structure and function. Previous studies give conflicting reports concerning the hdl platelet interaction. In our in vitro experiments, washed human blood platelets were preincubated with hdl (100 800 μg/ml) and then stimulated by serotonin (5 Hydroxytryptamine, 1 5 μM), a potent agonist and mediator in the process of atherosclerosis. Aggregatory studies and electron microscopy revealed that hdl could not prevent morphological alterations of blood platelets treated with serotonin. Furthermore, high dosages of hdl led to platelet activation. These findings indicate that hdl may not inhibit agonist induced platelet activation. As hdl is a heterogeneous group of different subclasses with opposite effects on platelet function, it Platelet Research Unit, Institute of Anatomy, University of Münster, Germany (Pfennig O and Dierichs R) Institute of Physiology, Ruhr University Bochum, Bochum, Germany (Liu B) can be concluded that hdl platelet interaction reflects relative concentrations of the particular sample. Our findings suggest that the protective influence of hdl may not be associated with decreased platelet function. Apoprotein E rich subclasses of hdl (hdl 2), particularly hdl enriched apoprotein E (hdl E), inhibit agonist induced platelet activation. In order to enlighten the role of apo E in this process, platelets in suspensions were preincubated with purified apoprotein E (50 400 μg/ml) and then stimulated by serotonin (5 μM). Apoprotein E of 300 μg/ml prevented morphological alterations of blood platelets and suppressed serotonin induced activation. Lower concentrated apoprotein E showed no clear effects. There is evid

关键词： hdl The influence of hdl and purified apoprotein E on platelet activation induced by serotonin

维普期刊数据库

如何在FPGA或ASIC设计中用TCL为hdl模型搭建自动验证平台

在线全文

学校读者我要写书评

暂无评论

如何在FPGA或ASIC设计中用TCL为HDL模型搭建自动验证平台

基于层次化设计的Verilog hdl增量编译方法

全国第一届嵌入式技术联合学术会议

作者：陈小龙荆涛北京交通大学电子信息工程学院

随着芯片复杂程度的增加,快速搭建一个强大、高效、灵活、可扩展性好的验证平台是芯片设计成功的关键因素之一。介绍了一种基于文件的层次化验证平台,使用TCL(tool command language)脚本语言来构建,因此在方法学上有一定的通用性。

关键词： FPGA ASIC TCL hdl 测试激励

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

计算机工程与应用 2003年第22期39卷 9-11页

作者：胡燕翔刘明业北京理工大学ASIC研究所北京100081

根据自顶向下设计方法和使用Veriloghdl设计层次化的特点,提出依据设计层次、以模块为单位的Veriloghdl增量编译方法。利用在语法分析和语义检查时构建的设计层次信息和符号表,根据被修改模块在设计层次中的位置,自动对全部相关模块进... 详细信息

根据自顶向下设计方法和使用Veriloghdl设计层次化的特点,提出依据设计层次、以模块为单位的Veriloghdl增量编译方法。利用在语法分析和语义检查时构建的设计层次信息和符号表,根据被修改模块在设计层次中的位置,自动对全部相关模块进行增量编译,同时更新编译结果和重构层次引用关系。在设计Veriloghdl编译器的同时予以实现。通过测试证明对于多模块设计中个别模块的修改,使用增量编译可以显著缩短重新编译的时间开销。

关键词：增量编译 IC Verilog hdl 模块设计层次

维普期刊数据库

Verilog hdl程序设计实例详解 1

在线全文

学校读者我要写书评

暂无评论