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In vitro effects of polyphenols on colorectal cancer cells

World Journal of Gastrointestinal Oncology 2014年第8期6卷 289-300页

作者： Barbara Pampaloni Gaia Palmini Carmelo Mavilia Roberto Zonefrati Annalisa Tanini Maria Luisa Brand Department of Surgery and Translational Medicine University of Florence Florence 50139 Italy

AIM:To investigate the effects of quercetin and genistein on colon cancer cell proliferation and their estrogen receptorβ(ERβ)***:Colon cancer cells were stably transfected with a mammalian expression vector to overexpress ERβ(hct8-β8-expressing cells)or a control vector(hct8-pSV2neo-expressing cells).The proliferation of these cells was examined after treatment with quercetin or genistein(5-100μmol/L),or 10 nmol/L17β-estradiol(17β-E2).Cell viability was examined by acridine orange staining following treatments for 48 or144 *** of quercetin and genistein on ERβtranscriptional transactivation were examined by luciferase activity in hct8-β8-expressing cells transiently transfected with a pEREtkLUC reporter *** addition,the regulation of ERβtranscription by phytoestrogens and 17β-E2 was examined by quantitative polymerase chain ***:Proliferation of hct8-β8-expressing cells was not reduced low doses(5μmol/L)of quercetin and genistein,while it was reduced at 25-50μmol/L with an effect similar to 10 nmol/L 17β-*** with doses of phytoestrogens≥75μmol/L completely blocked cell growth and reduced overall cell counts,however no effects at any dose were observed in hct8-pSV2neoexpressing *** results were supported by viability staining that revealed acridine orange-stained lysosomes with high doses or extended treatment *** and quercetin(50μmol/L)significantly increased ER-responsive luciferase activity similar to 10nmol/L 17β-E2(Phct8-β8-expressing cells(Phct8-pSV2neo-expressing cells with 50μmol/L quercetin or 10 nmol/L 17β-E2 significantly increased ERβmRNA levels compared to untreated controls(Phct8-β8-expressing ***:The antitumorigenic effects of the phytoestrogenic compounds querc

关键词： Estrogen receptor hct8-β8 cells hct8pSV2neo Quercetin Genistein

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