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Twist Promotes the Migration of Hepatocellular Carcinoma Cells

Clinical oncology and cancer researeh 2009年第5期6卷 313-316页

作者： Haibo Han Yantao Du Zhiqian Zhang Zhao Wei Clinical Laboratory Peking University School of Oncology Beijing Institute for Cancer Research Beijing 100142 China.

OBJECTIVE To study the effect of the Twist gene on the migration of human hepatocellular carcinoma cells and the possible mechanisms involved. METHODS RT-PCR was used to detect expression of the Twist gene in primary （Hep11） and recurrent （Hep12） cell lines from the same hcc.patient. Hep11 cells were stably transfected with Twist-cDNA, and Hep12 cells were transiently transfected with Twist RNAi plasmid. Cell migration assays were performed on Twist up-regulated Hep11 cells and Twist RNAi Hep12 cells. RT- PCR and Western blot were used to test the expression of EMT markers. RESULTS Twist was expressed higher level and had increased migration capability in recurrent Hep12 cells than those in primary Hep11 cells. Cell models （Twist-Hep11） in which Twist protein was steadily and highly expressed were obtained. Compared with pcDNA3-Hep11 cells, migration of Twist-Hep11 cells was clearly increased. However, migration of Twist RNAi （Si-Twist-Hep12） Hep12 cells were reduced. Overexpression of Twist in Hep11 cells promoted expression of N-cad and vimentin. CONCLUSION These results indicate that Twist promotes the migration of hepatocellular carcinoma cells in vitro and may play an important role in the upregulation of mesenchymal markers.

关键词： Twist RNA interference gene transfection migration and invasion hcc.

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