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Expression,purification and serological analysis of hepatocellular carcinoma associated antigen hca587 in insect cells

引用

World Journal of Gastroenterology 2003年第4期9卷 678-682页

作者： Bing Li Hong-Yan Wu Xiao-Ping Qian Yan Li Wei-Feng Chen Immunology Department of Peking University Health Science Center,Beijing,100083,China Immunology Department of Peking University Health Science Center 38 Xueyuan Road Beijing 100083 China. wfchen@***

AIM: In order to assess hepatocellular carcinoma associated antigen hca587 as a potential target for immunotherapy,the Bac-to-Bac expression system was used to express recombinant protein hca587 in insect ***: The cDNA encoding hca587 gene was cloned into donor vector pFasBacHtb and recombinant pFasBac Htb587 was transformed into competent cells *** Bacmid-587 was transfected into Sf9 insect cells using CELLFECTIN, Recombinant hca587 protein was produced in Sf9 insect cells after infection with recombinant baculovirus, and was purified using Ni-NTA resin. Sera from HCC patients were also screened using recombinant protein ***: The molecular weight of the recombinant protein hca587 expressed in insect cells was approximately *** blot results proved the recombinant protein hca587had the similar antigenicity with its native *** analysis told that the rate of seroreactivity to hca587 was not high in HCC ***: The recombinant protein hca587 was successfully expressed and purified using Bac-to-Bac expression system. It paved the way for generation of specific antibody and investigation of immunohistochemical analysis and immune responses of HCC in the future.

关键词： hca587抗原肝细胞癌血清学检查免疫疗法免疫组织化学

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