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Protective efficacy of an h5/h7 trivalent inactivated vaccine(h5-Re13,h5-Re14, and h7-Re4 strains) in chickens, ducks, and geese against newly detected h5N1, h5N6, h5N8, and h7N9 viruses

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Journal of Integrative Agriculture 2022年第7期21卷 2086-2094页

作者： ZENG Xian-ying hE Xin-wen MENG Fei MA Qi WANG Yan BAO hong-mei LIU Yan-jing DENG Guo-hua ShI Jian-zhong LI Yan-bing TIAN Guo-bin ChEN hua-lan State Key Laboratory of Veterinary Biotechnology National Poultry Laboratory Animal Resource CenterHarbin Veterinary Research InstituteChinese Academy of Agricultural SciencesHarbin 150069P.R.China

Some h5 viruses isolated in poultry or wild birds between 2020 and 2021 were found to be antigenically different from the vaccine strains(h5-Re11 and h5-Re12) used in China. In this study, we generated three new recombinant vaccine seed viruses by using reverse genetics and used them for vaccine production. The vaccine strain h5-Re13 contains the hemagglutinin(hA) and neuraminidase(NA) genes of an h5 N6 virus that bears the clade 2.***.4.4 h hA gene, h5-Re14 contains the hA and NA genes of an h5 N8 virus that bears the clade 2.***.4.4 b hA gene, and h7-Re4 contains the hA and NA genes of h7 N9 virus detected in 2021. We evaluated the protective efficacy of the novel h5/h7 trivalent inactivated vaccine in chickens, ducks, and geese. The inactivated vaccine was immunogenic and induced substantial antibody responses in the birds tested. Three weeks after vaccination, chickens were challenged with five different viruses detected in 2020 and 2021: three viruses(an h5 N1 virus, an h5 N6 virus, and an h5 N8 virus) bearing the clade 2.***.4.4 b hA gene, an h5 N6 virus bearing the clade 2.***.4.4 h hA gene, and an h7 N9 virus. All of the control birds shed high titers of virus and died within 4 days post-challenge, whereas the vaccinated chickens were completely protected from these viruses. Similar protective efficacy against h5 viruses bearing the clade 2.***.4.4 h or 2.***.4.4 b hA gene was observed in ducks and geese. Our study indicates that the newly updated h5/h7 vaccine can provide solid protection against the h5 and h7 N9 viruses that are currently circulating in nature.

关键词： avian influenza h5/h7 trivalent vaccine h5-Re13 h5-Re14 h7-Re4 strains protective efficacy chickens ducks and geese

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Protective efficacy of an h5/h7 trivalent inactivated vaccine produced from Re-11, Re-12, and h7-Re2 strains against challenge with different h5 and h7 viruses in chickens

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Journal of Integrative Agriculture 2020年第9期19卷 2294-2300页

作者： ZENG Xian-ying ChEN Xiao-han MA Shu-jie WU Jiao-jiao BAO hong-mei PAN Shu-xin LIU Yan-jing DENG Guo-hua ShI Jian-zhong ChEN Pu-cheng JIANG Yong-ping LI Yan-bing hU Jing-lei LU Tong MAO Sheng-gang GUO Xing-fu LIU Jing-li TIAN Guo-bin ChEN hua-lan State Key Laboratory of Veterinary Biotechnology Harbin Veterinary Research InstituteChinese Academy of Agricultural SciencesHarbin 150069P.R.China harbin Weike Biotechnology Development Company Harbin 150069P.R.China

We developed an h5/h7 trivalent inactivated vaccine by using Re-11, Re-12, and h7-Re2 vaccine seed viruses, which were generated by reverse genetics and derived their hA genes from A/duck/Guizhou/S4184/2017(h5N6) (DK/GZ/S4184/17) (a clade 2.***.4.4d virus), A/chicken/Liaoning/SD007/2017(h5N1) (CK/LN/SD007/17) (a clade 2.***.2.1d virus), and A/chicken/ Guangxi/SD098/2017(h7N9) (CK/GX/SD098/17), respectively. The protective efficacy of this novel vaccine and that of the recently used h5/h7 bivalent inactivated vaccine against different h5 and h7N9 viruses was evaluated in chickens. We found that the h5/h7 bivalent vaccine provided solid protection against the h7N9 virus CK/GX/SD098/17, but only 50–60% protection against different h5 viruses. In contrast, the novel h5/h7 trivalent vaccine provided complete protection against the h5 and h7 viruses tested. Our study underscores the importance of timely updating of vaccines for avian influenza control.

关键词： avian influenza h5/h7 trivalent vaccine Re-11 Re-12 and h7-Re2 strains protective efficacy

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