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Peritoneal fluid indocyanine green test for diagnosis of gut leakage in anastomotic leakage rats and colorectal surgery patients

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World Journal of Gastrointestinal Surgery 2024年第6期16卷 1825-1834页

作者： Yu Huang Tian-Yang Li Jie-Feng Weng Hui Liu Yu-Jie Xu Shuai Zhang Wei-Li Gu Department of Surgery Guangzhou First People's HospitalGuangzhou 510180Guangdong ProvinceChina

BACKGROUND Application of indocyanine green(ICG)fluorescence has led to new developments in gastrointestinal ***,little is known about the use of ICG for the diagnosis of postoperative gut leakage(GL).In addition,there is a lack of rapid and intuitive methods to definitively diagnose postoperative *** To investigate the effect of ICG in the diagnosis of anastomotic leakage in a surgical rat GL model and evaluate its diagnostic value in colorectal surgery *** Sixteen rats were divided into two groups:GL group(n=8)and sham group(n=8).Approximately 0.5 mL of ICG(2.5 mg/mL)was intravenously injected *** peritoneal fluid was collected for the fluorescence test at 24 and 48 *** patients with rectal cancer who had undergone laparoscopic rectal cancer resection plus enterostomies were injected with 10 mL of ICG(2.5 mg/mL)on postoperative day *** ostomy fluids were collected 24 h after ICG injection to identify the possibility of the ICG excreting from the peripheral veins to the enterostomy *** who had undergone colectomy or rectal cancer resection were enrolled in the diagnostic *** peritoneal fluids from drainage were collected 24 h after ICG *** ICG fluorescence test was conducted using OptoMedic endoscopy along with a near-infrared fluorescent imaging *** The peritoneal fluids from the GL group showed ICG-dependent green fluorescence in contrast to the sham *** samples of ostomy fluids showed green fluorescence,indicating the possibility of ICG excreting from the peripheral veins to the enterostomy stoma in *** peritoneal fluid ICG test exhibited a sensitivity of 100%and a specificity of 83.3%for the diagnosis of *** positive predictive value was 71.4%,while the negative predictive value was 100%.The likelihood ratios were 6.0 for a positive test result and 0 for a negative *** The postoperative ICG test in a drainage tube is a valuable and simple t

关键词： gut leakage Indocyanine green Anastomotic leakage model Diagnostic test Diagnostic technique

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Poultry gut health and beyond

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Animal Nutrition 2023年第2期13卷 240-248页

作者： Richard Ducatelle Evy Goossens Venessa Eeckhaut Filip Van Immerseel Livestock gut Health Team Department of PathobiologyPharmacology and Zoological MedicineFaculty of Veterinary MedicineGhent UniversityBelgium

Intestinal health is critically important for the digestion and absorption of nutrients and thus is a key factor in determining *** health issues are very common in high performing poultry lines due to the high feed intake,which puts pressure on the physiology of the digestive *** nutrients which are not digested and absorbed in the small intestine may trigger dysbiosis,i.e.a shift in the microbiota composition in the intestinal *** as well as other stressors elicit an inflammatory response and loss of integrity of the tight junctions between the epithelial cells,leading to gut *** this paper,key factors determining intestinal health and the most important nutritional tools which are available to support intestinal health are reviewed.

关键词： Poultry Intestinal health Inflammation gut leakage Microbiota Feed additive

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