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Vertical distribution of phosphorus and P-dissolving/ decomposing bacteria in the sediment of guanting reservoir

Science China Earth Sciences 2005年第Z2期48卷 285-294页

作者： LI Cui, YUAN Hongli & HUANG Huaizeng College of Biological Sciences, Key Laboratory of Agro-Microbial Resource and Application, Ministry of Agriculture, China Agricultural University, Beijing 100094, China National Research Center of Geoanalysis, Beijing 100037, China College of Biological Sciences Key Laboratory of Agro-Microbial Resource and Application Ministry of Agriculture China Agricultural University Beijing 100094 China National Research Center of Geoanalysis Beijing 100037 China

In this study, we determined pH, phosphorus content and the number of P-dis- solving/decomposing bacteria in relation to the depth in the sediment of guanting reservoir in Beijing. The pH value was slightly increased from 8.0 in the surface to 8.5 in the bottle (69 cm in depth) of sediment. The highest P content, 1269 mg/kg, was detected in the 35 cm layer, followed by the 5 cm (993 mg/kg) and 69 cm (580 mg/kg) layers. The number of inorganic P-dissolving bacteria varied from 6(102 to 8(104 and the organic P-discomposing bacteria were from 1.9(103 to 6.3(104 per gram sediment in different depths, which were counted under 28℃ and 20℃ ( pH7.5 and 8.5. The number of P-dissolving/discomposing bacteria was directly correlated to the P content in each layer of sediments. The analysis of P-dissolving/decomposing ability of bacteria showed that some of these bacteria were also capable of accumulating P intracellularly. The intracellular P-accumulation was more efficient at lower temperature; in contrast, the activity of P-dissolving/decomposing was stronger at higher temperature. So the content of dissolved P in water body, or quality of water, could be affected by the change of temperature via the regulation of bacterial activity. On the basis of 16S rDNA sequencing, the 13 efficient P-dis- solving/decomposing bacteria were identified as Bacillus spp., Bacterium sp., Microbacterium sp., Paenibacillus sp. and Pseudomonas sp.

关键词： guanting reservoir, sediment, P-dissolving/decomposing bacteria, phosphorus cycling, eutrophication.

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Science China Earth Sciences 2005年第Z2期48卷 276-284页

作者： QU Jianhang, YUAN Hongli, HUANG Huaizeng & WANG Entao College of Biological Sciences, Key.Laboratory of Agro-Microbial Resource and Application, Ministry of Agriculture, China Agricultural University, Beijing 100094, China National Research Center of Geoanalysis, Beijing 100037, China Escuela Nacional de Ciencias Bioldgicas, Instituto Politecnico Nacional, Mexico D. F. 11340, Mexico College of Biological Sciences Key Laboratory of Agro-Microbial Resource and Application Ministry of Agriculture China Agricultural University Beijing 100094 China National Research Center of Geoanalysis Beijing 100037 China Escuela Nacional de Ciencias Biológicas Instituto Politécnico Nacional México D. F. 11340 México

In this study, DNAs were extracted from sediment samples at depths of 5, 35, and 69 cm from eutrophic guanting reservoir, China. 16S rDNAs were amplified by PCR and clone libraries were constructed. The depth-related distribution of bacterial community in the sediment was characterized by using amplified 16S rDNA restriction analysis (ARDRA) and sequencing of the dominant clones. The results indicated that species diversity in the sediment of guanting reservoir was rather high with the Shannon-Wiener index about 5.8. Bacterial richness varied in different depths: the highest in the sample of 35 cm in depth; followed by the sample of 5 cm in depth; and the lowest bacterial richness in the sample of 69 cm. Dominant species from the three samples were different although there were some common clones. PhyIogenetic analysis showed that all of the dominant clones in the three layers were uncultured bacteria and distantly related to the previously reported species in beta or gamma subclass of proteobacteria, including bacterial groups that have the ability to degrade aromatic hydrocarbons, n-all:anes, chlorinated organic compounds, or to accumulate polyphosphate, etc. Changes of deptt-related bacterial community in the guanting reservoir sediment might reflect the pollution history and the water quality of the reservoir. In addition, the cloned sequences from the guanting reservoir sediment were all different from the presently reported ones, indicating that there were some particular bacteria in that environment.

关键词： guanting reservoir, sediment, bacterial community, 16S rDNA, eutrophication.

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Bacterial Community Structure of Sediment in Upstream Ecological Wetland of guanting Reservoir under the Action of Different Plants in Winter

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Meteorological and Environmental Research 2019年第1期10卷 46-48,51页

作者： Yan Donghua Jiang Tiantian Li Hongbo Zhai Xuezheng Wang Mengyao Wang Jianghua Zhao Jianguo Institute of Green Development Hebei Provincial Academy of Environmental Sciences Institute of Water Environment Science Hebei Provincial Academy of Environmental Sciences Baoding Institute of Environmental Protection

In order to study the effect of reed and cattail on the bacterial community structure of surface sediments of wetland in winter,highthroughput sequencing technology was used to analyze the bacterial composition. The results showed that 134 228 high-quality gene sequences were obtained by high-throughput sequencing in sediments of natural wetland in winter,and there were 13 866 OTUs,in which the number of OUT in sediments of reed wetland and cattail wetland was 6 677 and 7 189 respectively. The richness and diversity of bacterial communities in sediments of cattail wetland in winter were higher than those in sediments of reed wetland. Relative abundance of Proteobacteria in sediments of reed wetland and cattail wetland was 0. 54 and 0. 52,respectively. The dominant classes of bacteria were Betaproteobacteria,Deltaproteobacteria,Gammaproteobacteria and Epsilonproteobacteria,which belonged to gram-negative bacteria,indicating that the rhizosphere of wetland was easier to enrich gram-negative bacteria. Under the genus level,the dominant bacteria in the sediments were not obvious.

关键词： High-throughput sequencing Bacterial community Sediment Winter Upstream ecological wetland of guanting Reservoir

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