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Biological characteristics of dynamic expression of nerve regeneration related growth factors in dorsal root ganglia after peripheral nerve injury

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Neural Regeneration Research 2020年第8期15卷 1502-1509页

作者： Yin-Ying Shen Xiao-Kun Gu Rui-Rui Zhang Tian-Mei Qian Shi-Ying Li Sheng Yi Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education Co-innovation Center of NeuroregenerationNantong UniversityNantongJiangsu ProvinceChina Department of Hand Surgery Affiliated Hospital of Nantong UniversityNantongJiangsu ProvinceChina

The regenerative capacity of peripheral nerves is limited after nerve injury.A number of growth factors modulate many cellular behaviors,such as proliferation and migration,and may contribute to nerve repair and *** previous study observed the dynamic changes of genes in L4–6 dorsal root ganglion after rat sciatic nerve crush using transcriptome *** current study focused on upstream growth factors and found that a total of 19 upstream growth factors were dysregulated in dorsal root ganglions at 3,9 hours,1,4,or 7 days after nerve crush,compared with the 0 hour ***-six rat models of sciatic nerve crush injury were prepared as described ***,they were divided into six groups to measure the expression changes of representative genes at 0,3,9 hours,1,4 or 7 days post *** current study measured the expression levels of representative upstream growth factors,including nerve growth factor,brain-derived neurotrophic factor,fibroblast growth factor 2 and amphiregulin genes,and explored critical signaling pathways and biological process through bioinformatic *** data revealed that many of these dysregulated upstream growth factors,including nerve growth factor,brain-derived neurotrophic factor,fibroblast growth factor 2 and amphiregulin,participated in tissue remodeling and axon growth-related biological processes Therefore,the experiment described the expression pattern of upstream growth factors in the dorsal root ganglia after peripheral nerve *** analysis revealed growth factors that may promote repair and regeneration of damaged peripheral *** animal surgery procedures were performed in accordance with Institutional Animal Care Guidelines of Nantong University and ethically approved by the Administration Committee of Experimental Animals,China(approval No.20170302-017)on March 2,2017.

关键词： axon growth bioinformatic analysis dorsal root ganglia growth factors Ingenuity Pathway Analysis nerve regeneration peripheral nerve injury rat sciatic nerve crush injury transcriptome sequencing upstream regulators

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comparison between tocotrienol and omeprazole on gastric growth factors in stress-exposed rats

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World Journal of Gastroenterology 2017年第32期23卷 5887-5894页

作者： Mohd Fahami Nur Azlina Hj Mohd Saad Qodriyah Kien Hui Chua Yusof Kamisah Department of Pharmacology Faculty of MedicineUniversiti Kebangsaan Malaysia Department of Physiology Faculty of MedicineUniversiti Kebangsaan Malaysia

AIM To investigate and compare the effects of tocotrienol and omeprazole on gastric growth factors in rats exposed to water-immersion restraint stress(WIRS).METHODS Twenty-eight male Wistar rats were randomly assigned to four groups of seven rats. The two control groups were administered vitamin-free palm oil(vehicle) and the two treatment groups were given omeprazole(20 mg/kg) or tocotrienol(60 mg/kg) by oral gavage. After 28 d of treatment, rats from one control group and both treated groups were subjected to WIRS one time for 3.5 h. Gastric lesions were measured and gastric tissues were obtained to measure vascular endothelial growth factor(VEGF), epidermal growth factor(EGF), basic fibroblast growth factor(b FGF), and transforming growth factor-alpha(TGF-α) m RNA expression. RESULTS Rats exposed to WIRS for 3.5 h demonstrated the presence of considerable ulcers in the form of gastric erosion. The lesion index in the stressed control(S) group was increased(P growth factors that assist in repair of gastric tissue injuries.

关键词： Tocotrienol Omeprazole Restraint-stress Gastric ulcers growth factors

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Analysis and Optimisation of Halomonas growth factors Based on PCA and RSM

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China Petroleum Processing & Petrochemical Technology 2019年第4期21卷 81-87页

作者： Shi Ke Huang Guofu Xu Huachun Xue Jianliang Sun Jingkuan Xiao Xinfeng Li Lin College of Chemical and Environmental Engineering Shandong University of Science and TechnologyQingdao 266590 Shandong Key Laboratory of Eco-Environmental Science for Yellow River Delta(Binzhou University) Bingzhou 256600 Shandong Peninsula Engineering Research Center of Comprehensive Brine Utilization Weifang University of Science and TechnologyShouguang 262700

The biomass of petroleum-degrading bacteria, such as Halomonas spp., is crucial to the alleviation of severe oil spills through bioremediation. In this paper, the bacterium(HDMP1) was isolated and identified. growth factors were analysed and optimised through the single-factor experiments, the factor analysis(FA), the principal component analysis(PCA), and the response surface methodology(RSM). Results indicated that HDMP1 was identified as genus Halomonas. In the single-factor experiments, the range of suitable growth conditions for HDMP1 covered: a salt concentration of 2%-4%, a medium pH value of approximately 9, an inoculum concentration of 1.0%, a substrate concentration of 1.0%-1.4%, and a rotation rate of 140 r/min. The evaluation by FA and PCA indicated that three significant growth factors were the salt concentration, the pH value, and the rotation rate. A maximum biomass of HDMP1 was obtained under the conditions covering a salt concentration of 3.5%, a medium pH of 8, and a rotation rate of 151 r/min by optimization.

关键词： biomass factor analysis principal component analysis growth factors response surface methodology

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Comprehensive assessment of growth factors,inflammatory mediators, and cytokines in vitreous from patients with proliferative diabetic retinopathy

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International Journal of Ophthalmology(English edition) 2022年第11期15卷 1736-1742页

作者： Qiao-Yun Gong Guang-Yi Hu Su-Qin Yu Tian-Wei Qian Xun Xu Department of Ophthalmology Shanghai General HospitalShanghai Jiao Tong UniversityShanghai 200080China National Clinical Research Center for Eye Diseases Shanghai 200080China Shanghai Key Laboratoryof Ocular Fundus Diseases Shanghai 200080China Shanghai Engineering Center for Visual Science and Photomedicine Shanghai 200080China Shanghai Engineering Center for Precise Diagnosis and Treatment of Eye Disease Shanghai 200080China

AIM: To assess alterations in growth factors, inflammatory mediators, and cytokines associated with vitreous-retinal diseases in vitreous humor from patients with proliferative diabetic retinopathy(PDR), and to identify potential new treatment targets and ***: Control vitreous samples were collected from patients with macular hole, epiretinal membranes, or rhegmatogenous retinal detachments, and PDR samples from patients with complications of PDR, who required pars plana vitrectomy. Specimens were stored at-80℃ and then investigated by Luminex multi-factor assay. Parametric and nonparametric analyses of demographic characteristics and cytokine expression levels were conducted using ***: There were no significant differences in demographic characteristics between patients with and without PDR. Expression levels of growth factors [plateletderived growth factor(PDGF)-AA, glial cell line-derived neurotrophic factor(GDNF), and vascular endothelial growth factor A(VEGFA)], inflammatory mediators [interleukin(IL)-8, IL-11, and tumor necrosis factor-α(TNF-α)] and cytokines [chemokine C-X-C ligand(CXCL)10, interferon-γ(IFN-γ), and granulocyte macrophage-colony stimulating factor(GM-CSF)] were significantly elevated in vitreous humor from patients with PDR compared with those in the control group(all P0.05).CONCLUSION: This proof-of-concept study demonstrates the potential for combinational therapeutic strategies to ameliorate diabetic retinopathy progression by targeting growth factors, inflammatory factors, and cytokines, in addition to VEGFA.

关键词： vitreous proliferative diabetic retinopathy growth factors inflammation cytokines

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Inflammatory priming of mesenchymal stem cells:Focus on growth factors enhancement

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BIOCELL 2022年第9期46卷 2049-2052页

作者： ALEKSANDRA GORNOSTAEVA ELENA ANDREEVA LUDMILA BURAVKOVA Cell Physiology Laboratory Institute of Biomedical Problems of Russian Academy of SciencesMoscow123007Russia

Multipotent mesenchymal stromal cells(MSCs)are actively involved in reparation and inflammation processes,providing damaged tissue reparation and suppressing immune cell responses in *** effects are mostly due to the production of a wide range of paracrine factors,including growth factors and immunomodulatory *** induce immunosuppressive activity,MSCs are primed by inflammatory cytokines,which results in an increased production of immunomodulatory ***,stimulation of reparative properties is also *** viewpoint manuscript highlights the possibilities of inflammatory priming to increase the production of growth factors by MSCs.

关键词： Multipotent mesenchymal stromal cells Priming growth factors

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IMMUNOELECTRON　MICROSCOPIC　LOCALIZATION　OF　growth　factors　AND　OTHER　MARKERS　IN　HUMAN　LONG－TERM　BONE　MARROW　CULTURES

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Chinese Medical Sciences Journal 1996年第3期11卷 129-135页

作者：刘杰文 Institute of Haemotology CAMS Tianjin.

Ultrastructural immunocytochemical characterization of human long-term bone marrow cultures has shown positive localization for growth factors on cell surface and on extracellular matrix(ECM).In some cases double-labelling indicated co-localization of growth factors and specific cell surface *** markers for endothelial cells and fibroblasts showed that growth factor(GM-CSF,G-CSF and b-FGF) were present at the surface of these cell *** scanning and transmission electron microscopy indicated intense labelling for growth factors on the extracellular ***-labelling of heparan sulphate proteoglycans and GM-CSF showed a co-localization of the labelling,which indicated the binding of growth factor to the extracellular matrix.

关键词： bone marrow growth factors specific markers

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Electroacupuncture at Ren channel point combined with injection of basic fibroblast growth factors for neural stem cell proliferation in subventricular zone of cerebral ischemic side

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Neural Regeneration Research 2006年第5期1卷 424-427页

作者： Min Pi Lihong Diao Haibo Yu Xiaodan Rao Ling Wang Yuansheng Liu Wenshu Luo Zhuoxin Yang Department of Acupuncture and Moxibustion Shenzhen Hospital Guangzhou University of Traditional Chinese Medicine Shenzhen 518033 Guangdong Province China Postdoctoral Station of Guangzhou University of Traditional Chinese Medicine Guangzhou 510405 Guangdong Province China

BACKGROUND： Electroacupuncture at Ren channel point can treat stroke and generate some neurodevelopment-related growth factors, which are associated with the proliferation and differentiation of stem cells. OBJECTIVE： To observe the effect of electroacupuncture at Ren channel point combined with injection of basic fibroblast growth factors （bFGF） on the expression of 5-bromo-2-deoxyuridine （BrdU）-Iabeled and BrdU/Neuroepthelial stem cell protein （Nestin）-Iabeled positive cells in subventricular zone of cerebral ischemic rats. DESIGN ： Completely randomized grouping design and controlled trial.SETTING： Department of Acupuncture and Moxibustion, Shenzhen Hospital, Guangzhou University of Traditional Chinese Medicine. MATERIALS ： Eighty-four adult healthy male Wistar rats were involved and divided into 5 groups by a lot： blank group （n =6）, sham-operation group （n =6）, model group （n =26）, injection of bFGF group （bFGF group, n =24） and electroacupuncture at Ren channel combined with injection of bFGF group （bFGF group, n =22）. Three time points were set in each of the latter three groups for observation： 7, 14 and 28 days after ischemia respectively. Mouse BrdU and Nestin monoclonal antibody （analytical pure, China）, G6805 electro-acupuncture apparatus （Huayi Medical Instrument, Shanghai） and Olympus FV 500 laser confocal microscope （Olympus Company） were used. METHODS ： This trial was carried out in the Laboratory of Anatomy, Medical College of Sun Yat-sen University from September 2004 to July 2005. ① Except for blank group and sham-operation group, the rats in the other groups were prepared into rat models of local cerebral ischemia by suture-occluded method. The rats were reperfused, and then they were graded in neurologic deficits based on neurological scoring of Longa. Rat model was successful when the neurological scoring ranged from 1 to 4 points and used for the trial. Blank group was untouched; Right common carotid artery was isolated and right exter

关键词： subventricular zone cell proliferation cerebral ischemia normal group Traditional Chinese Medicine growth factors common carotid artery Acupuncture and Moxibustion neural stem cells monoclonal antibody confocal microscope synergistic effect Guanyuan point Wistar rats time point

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Content of blood cell components,inflammatory cytokines and growth factors in autologous platelet-rich plasma obtained by various methods

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World Journal of Orthopedics 2022年第6期13卷 587-602页

作者： Maciej Dejnek Jarosław Witkowski Helena Moreira Sylwia Płaczkowska Piotr Morasiewicz PawełReichert Aleksandra Królikowska Department of Trauma Surgery Wroclaw Medical UniversityWroclaw 50-556Poland Department of Medical Science Foundation Wroclaw Medical UniversityWroclaw 50-556Poland Teaching and Research Diagnostic Laboratory Department of Laboratory DiagnosticsWroclaw Medical UniversityWroclaw 50-556Poland Institute of Medical Sciences Department of Orthopaedic and Trauma SurgeryUniversity of OpoleOpole 45-052Poland Ergonomics and Biomedical Monitoring Laboratory Department of PhysiotherapyWroclaw Medical UniversityWroclaw 50-355Poland

BACKGROUND The evaluation of the efficacy of platelet-rich plasma(PRP) in clinical practice yields conflicting results and raises numerous controversies. This may be due to different concentrations of biologically active components in PRP obtained with the use of different methods of gravity *** To compare the content, repeatability and correlations between biologically active components in PRP obtained with four different commercial *** From a whole blood sample of each of 12 healthy male volunteers, 4 PRP samples were prepared using 4 different commercial kits [Arthrex Autologous Conditioned Plasma(ACP), Mini GPS Ⅲ, Xerthra, Dr. PRP] in accordance with the instructions provided by the manufacturers. A comparative analysis of blood cell components-13 selected inflammatory cytokines and 7 growth factors-in the obtained PRP samples was performed using the Kruskal-Wallis test by ranks. The repeatability of results in each method was evaluated by the estimation of the coefficient of variation. The Spearman correlation was used to estimate the relationship between blood cell content and *** Significantly higher concentrations of platelets(PLT), white blood cells(WBC) and red blood cells(RBC) were found in PRP obtained with the use of Mini GPS Ⅲ than in PRP obtained using other systems. Significant differences in the content of growth factors and cytokines in PRP were found. A positive correlation of the amount of PLT, RBC and WBC with the concentration of most of the growth factors was found but in only three inflammatory cytokines. The obtained correlations between blood cell components and cytokines differed between the systems in terms of statistical significance, which may be due to insufficient sample size. The repeatability of the obtained PLT concentration also varied between protocols with the lowest in Xerthra and the highest in Arthrex *** Significant differences in the content of biologically active components a

关键词： Platelet-rich plasma Cytokines Chemokines growth factors

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growth factors and Supporting Cells of Nerve Conduits for Peripheral Nerve Regeneration

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Chinese Journal of Plastic and Reconstructive Surgery 2019年第4期1卷 46-54页

作者： Yang XIANG Zhi-Wu CHEN Jun-Shui ZHENG Zhuan YANG Guang-Hao LIN Peng WEI Department of Plastic and reconstructive surgery Ningbo First HospitalNingbo CityZhejiang Province315010China Ningbo university Ningbo CityZhejiang Province315010China

Peripheral nerve injury is a common disease that endangers human *** is a variety of methods to repair peripheral nerve injury,the current"gold standard"approach is autograft,however it still faces many disadvantages.A new choice is the use of artificial nerve conduits,which are tubular structures and are designed to bridge nerve *** order to bridge longer nerve gaps and gain ideal nerve regeneration effects,multiple technologies have been developed to the design of nerve conduits,such as selecting sutible materials,supplementing growth factors,transplanting supporting cells and so *** review mainly introduce current progess in growth factors supplementation and supporting cells transplantation technology of nerve conduits.

关键词： peripheral nerve injury nerve conduits growth factors supporting cells

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Detecting cell-secreted growth factors in microfluidic devices using bead-based biosensors

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Microsystems & Nanoengineering 2017年第1期3卷 242-250页

作者： Kyung Jin Son Pantea Gheibi Gulnaz Stybayeva Ali Rahimian Alexander Revzin Department of Biomedical Engineering University of CaliforniaDavisCalifornia 95616USA Department of Physiology and Biomedical Engineering Mayo ClinicRochesterMinnesota 55905USA

Microfluidic systems provide an interesting alternative to standard macroscale cell cultures due to the decrease in the number of cells and reagents as well as the improved physiology of cells confined to small ***,the tools available for cell-secreted molecules inside microfluidic devices remain *** this paper,we describe an integrated microsystem composed of a microfluidic device and a fluorescent microbead-based assay for the detection of the hepatocyte growth factor(HGF)and the transforming growth factor(TGF)-β1 secreted by primary *** microfluidic system is designed to separate a cell culture chamber from sensing chambers using a permeable hydrogel ***-secreted HGF and TGF-β1 diffuse through the hydrogel barrier into adjacent sensing channels and are detected using fluorescent microbead-based *** specificity of sensing microbeads is defined by the choice of antibodies;therefore,our microfluidic culture system and sensing microbeads may be applied to a variety of cells and cell-secreted factors.

关键词： biosensors growth factors microbead microchamber primary hepatocytes

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