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growth inhibition of high-intensity focused ultrasound on hepatic cancer in vivo

World Journal of Gastroenterology 2005年第28期11卷 4317-4320页

作者： Xiu-Jie Wang Shu-Lan Yuan Yan-Rong Lu Jie Zhang Bo-Tao Liu Wen-Fu Zeng Yue-Ming He Yu-Rui FU Division of Experimental Oncology Key Laboratory of Biotherapy of Human Diseases of Ministry of Education PR China West China Hospital Sichuan University. Chengdu 610041. Sichuan Province. China Mianyang Electronic Equipment Factory Mianyang 621000 Sichuan ProvinceChina

AIM： To investigate the damaging effect of high-intensity focused ultrasound （HIFU） on cancer cells and the inhibitory effect on tumor growth. METHODS： Hurine H22 hepatic cancer cells were treated with HIFU at the same intensity for different lengths of time and at different intensities for the same length oftime in vitro, the dead cancer cells were determined by trypan blue staining. Two groups of cancer cells treated with HIFU at the lowest and highest intensity were inoculated into mice. Tumor masses were removed and weighed after 2 wk, tumor growth in each group was confirmed pathologically.RESULTS： The death rate of cancer cells treated with HIFU at 1 000 W/cm^2 for 0.5, 1, 2, 4, 8, and 12 s was 3.11±1.21%, 13.37±2.56%, 38.84±3.68%, 47.22±5.76%,87.55±7.32%, and 94.33±8.11%, respectively. A positive relationship between the death rates of cancer cells and the length of HIFU treatment time was found （r = 0.96,P〈0.01）. The death rate of cancer cells treated with HIFU at the intensity of 100, 200, 400, 600, 800, and 1 000 W/cm^2 for 8 s was 26.31±3.26%, 31.00±3.87%, 41.97±5.86%,72.23±8.12%, 94.90±8.67%, and 99.30±9.18%, respectively. A positive relationship between the death rates of cancer cells and the intensities of HIFU treatment was confirmed （r= 0.98, P〈0.01）. The cancer cells treated with HIFU at 1 000 W/cm^2 for 8 s were inoculated intomice ed into. The tumor inhibitory rate was 90.35% compared to the control （P〈0.01）. In the experimental group inoculated with the cancer cells treated with HIFU at 1 000 W/cm^2 for 0.5 s, the tumor inhibitory rate was 22.9% （P〈0.01）. By pathological examination, tumor growth was confirmed in 8 out of 14 mice （57.14%, 8/14） inoculated with the cancer cells treated with HIFU at 1 000 W/cm^2 for 8 s, which was significantly lower than that in the control （100%, 15/15, P〈O.05）.CONCLUSION： HIFU is effective on killing or damage of H22 hepatic cancer cells in vitro and on inhibiting tumor growth in mice ex vivo.

关键词： HIFU Uver cancer growth inhibition

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growth inhibition of Breast Cancer in Rat by AAV Mediated Angiostatin Gene

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Chinese Journal of Cancer Research 2007年第2期19卷 108-112页

作者：李然陈红任常山 Cancer Research Institute The First Affiliated Hospital China Medical University

Objective： To observe growth inhibition effect of adeno-associated viral vectors （AAV） mediated angiostatin （ANG） gene on implanted breast cancer in rat and its mechanism. Methods： Gene transfer technique was used to transfer AAV-ANG to the tumor. growth curves were drawn to observe the growth of breast cancer implanted in rat, and immunohistochemical method was used to detect the effects of angiostatin on microvesel density （MVD） of breast cancer implanted in rat. Results： Angiostatin inhibited the growth of breast cancer implanted in rat and decreased the microvessel density of tumor. Conclusion： Expression of an angiostatin transgene can suppress the growth of breast cancer implanted in rat through the inhibition of the growth of microvessels, surggesting that angiostatin gene transfer technique may be effective against breast cancer.

关键词： AAV-ANG Breast cancer growth inhibition Microvessel density

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growth inhibition to three red tide microalgae by extracts of Ulva pertusa

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Chinese Journal of Oceanology and Limnology 2006年第2期24卷 147-153页

作者：金秋董双林王长云 Mariculture Laboratory College of Fisheries Ocean University of China Qingdao 266003 China Institute of Marine Drug and Food Ocean University of China Qingdao 266003 China

growth inhibition effect of different concentration of distilled water extract and four polar organic solvent (methanol, acetone, ether and chloroform) extracts of Ulva pertusa on three typical red tide microalgae (Heterosigma akashiwo, Alexandrium tamarense and Prorocentrum micans) were inves- tigated. Liquid-liquid fractionation and HPLC analysis for methanol extract of U. pertusa were carried out. growth of the three microalgae was significantly inhibited by the distilled water extract of U. pertusa at relatively higher concentration. However, the cells of the three microalgae did not die completely even at high concentration. Methanol extract of U. pertusa showed the highest growth inhibition on the three mi- croalgae, and all the cells of the three microalgae were killed at relatively high concentration. The other three organic solvent extracts of U. pertusa had no apparent effect on the three microalgae. The results of bioassays and HPLC analysis suggested that the inhibitory substances in U. pertusa to the microalgal growth had relatively high polarities. H. akashiwo was the most sensitive one while A. tamarense was the most tolerant one to the growth inhibitory substances.

关键词： growth inhibition extracts Ulva pertusa Heterosigma akashiwo Alexandrium tamarense Prorocentrum micans

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Lentivirus mediated shRNA interference targeting MAT2B induces growth-inhibition and apoptosis in hepatocelluar carcinoma

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World Journal of Gastroenterology 2008年第29期14卷 4633-4642页

作者： Qun wang Quan-Yan Liu Zhi-Su Liu Qun Qian Quan Sun Ding-Yu Pan Department of General Surgery Zhongnan Hospital Wuhan University Wuhan 430071 Hubei Province China

AIM: To investigate the effects of lentivirus vector mediated short hairpin RNA interference targeting methionine adenosyltransferase 2β gene (LV-shMAT2B) on hepatocelluar carcinoma (HCC) cells. METHODS: We constructed four plasmids of RNA interference targeting the MAT2B gene. After LV-shMAT2B was transfected with L-02 cells and two kinds of HCC cells, cell viability and proliferation were measured with MTT and [3H]thymidine assays respectively. Flow cytometry was used to assess cell apoptosis. The level of S-adenosyl methionine (SAMe) in HepG2 cells was evaluated. The expressions of cyclin D1, cyclin D2, bcl-xL and bcl-xS were detected with western blot. RESULTS: We constructed LV-shMAT2B successfully. LV-shMAT2B was safe for human normal liver cells. LV-shMAT2B caused dramatic reduction in proliferation compared with controls in HCC cells Bel-7402 (P = 0.054) and HepG2 (P = 0.031). Flow cytometry analysis showed that cell apoptosis caused by LV-shMAT2B was greater in HCC cells Bel-7402 and HepG2 than in control induced by scrambled siRNA (P = 0.047), but apoptosis rates in L-02 induced by LV-shMAT2B and scrambled siRNA respectively had no significant difference. Moreover, LV-shMAT2B significantly suppressed expression of MAT2B leading to growth-inhibition effect on HCC cells by down-regulating cyclin D1. Apoptosis induced by LV-shMAT2B was involved indown-regulating bcl-xL and up-regulating bcl-xS. CONCLUSION: LV-shMAT2B can induce cell apoptosis and growth-inhibition in HCC cells. MAT2B may be a therapy target in HCC in the future.

关键词： Lentivirus Methionine adenosyltransferase2β gene growth inhibition Apoptosis Hepatocelluarcarcinoma

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growth inhibition and Apoptosis Induced by Retinoic Acid Combined with Interferon Alpha-2a on Transitional Cell Carcinoma of Bladder

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Journal of Nanjing Medical University 2004年第3期18卷 158-162页

作者： QIANLi-xin LIUXun-liang ZHOUJian-wei MonicaLiebert ZOUChang-chun ZOUChang-ping DepartmentofUrology theFirstAffiliatedHospitalofNanjingMedicalUniversityNanjing210029 InstituteofAppliedToxicology NanjingMedicalUniversityNanjing210029P.R.China AmericanUrologyAssociation DepartmentofOB／Gyn TheUniversityofArizonaTucsonArizona85724USA

Objective:To identify new favorable agents and develop novel approaches for the chemoprevention and treatment of superficial bladder cancer and investigate the effects of combination of retinoids and interferon α-2a on growth inhibition and apoptosis induction in bladder cancer cell lines. Methods:Four bladder cancer cell lines,grade 1 to 3,and two retinoids,all-trans-retinoic acid(ATRA),9-cis retinoic acid(9cRA),combined with interferon α-2a(INF),were used in the *** compared the competence of these agents to inhibit growth,induce apoptosis,affect the expression of nuclear retinoid receptors,and modulate STAT1 protein. Results: Most of the bladder cancer cell lines were resistant to the effect of ATRA and 9cRA on growth inhibition and apoptosis induction,even at higher concentration(10 -5M).The effects of ATRA and 9c RA on cell growth and apoptosis were enhanced by INF α- 2a. Combination of ATRA and IFNα-2a induced RARβ and Stat 1 expression in three bladder cancer cell lines. Conclusion:The results demonstrated that INFα-2a synergize with the inhibitory effect of ATRA and 9c RA on the growth inhibition and apoptosis of bladder cancer cells in vitro,which suggested that it has a potential interest for the treatment of transitional cell carcinoma of bladder.

关键词： apoptosis growth inhibition retinoids interferon bladder cancer cell

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inhibition of human gastric carcinoma cell growth by atofluding derivative N_3-o-toluyl-fluorouracil

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World Journal of Gastroenterology 2006年第42期12卷 6766-6770页

作者： Jian Liu Wen-Fang Xu Shu-Xiang Cui Yong Zhou Yun-Xia Yuan Ming-Hui Chen Ruo-Han Wang Ruo-Yan Gai Masatoshi Makuuchi Wei Tang Xian-Jun Qu Department of Pharmacology School of Pharmaceutical Sciences Shandong University Jinan 250012 Shandong Province China Department of Pharmacy Second Affiliated Hospital Shandong Traditional Chinese Medical University Jinan 250001 Shandong Province China Department of Pharmacology The Institute of Materia Medica Shandong Academy of Medical Sciences Jinan 250062 Shandong Province China Center for Drug Evvaluation and Certification of Shandong Jinan 250014 Shandong Province China Hepato-Biliary-Pancreatic Surgery Division Department of Surgery Graduate School of Medicine The University of Tokyo 113-8655 Tokyo Japan

AIM： To evaluate the growth inhibition efficacy of atofluding derivative N3-o-toluyl-fluorouracil （TFU） on human gastric carcinoma cell lines SGC-7901 and MKN-45. METHODS： Cell growth inhibition by TFU was measured by MTT and clonogenic assays without or with liver microsomal enzymes. Xenografts of cancer cells in nude mice were employed to study the anti-proliferative effects of TFU in vivo. RESULTS： TFU inhibited the growth of SGC-7901 and MKN-45 cells. However, the inhibitory effects of TFU on cell growth were not significant. The inhibition rates were enhanced in the presence of liver microsomal enzymes, ranging 4.73%-48.57% in SGC-7901 cells and 9.0%-62.02% in MKN-45 cells. In v/vo, TFU delayed the growth of SGC-7901 and MKN-45 cells in nude mice. The inhibition rates were 40.49%, 63.24%, and 75.98% in SGC-7901 cells and 40.76%, 61.41%, and 82.07% in MKN-45 cells when the oral doses were 25, 50, and 100 mg/kg, respectively. TFU treatment was generally well tolerated by mice with less than 20% reduction in body weight. CONCLUSION： TFU inhibits the growth of human gastric carcinoma cells. The inhibition rates are increased in the presence of liver microsomal enzymes. The efficacy of TFU may be associated with the sustaining release of 5-fluorouracil （5-FU） mediated by the enzymes.

关键词： N3-o-toluyl-fluorouracil Gastric carcinoma cells Pro-drug growth inhibition

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inhibition of the growth and development of mosquito larvae of Culex quinquefasciatus(Diptera:Culicidae) treated with extract from leaves of Pseudocalymma alliaceum(Bignonaceae)

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Asian Pacific Journal of Tropical Medicine 2014年第8期7卷 594-601页

作者： Carlos Granados-Echegoyen Rafael Pérez-Pacheco Marcos Soto-Hernndez Jaime Ruiz-Vega Luicita Lagunez-Rivera Nancy Alonso-Hernandez Rene Gato-Armas CIIDIR-OAXACA Instituto Poluécnico NacionalCalle Hornos 1003Santa CruzXoxocotlnOaxacaMéxico.C.P.71230 Colegio de Postgraduados MontecilloEstada de MéxicoMexico Institute de Medicina Tropical "Pedro Kouri" La HabanaCuba

Objective:To determine larvicidal activity of the essential oil,hydrolat and botanical extracts derived from leaves ol Pseudocalymma alliaceum an mosquito larvae of Culex ***:Groups of twenty lanae were used in the larvicidal *** mortality,relative growth rate,the larval and pupal duration and viability was *** essential oil was analyzed by solid phase microextraction using gas chromatography coupled to mass ***:Essential oil at 800 ppm showed larvicidal activity at 24 h with lethal values of LC_(50) and LC_(90) of 267.33 and 493.63 *** hvdrolat at 20% and 10% on 2nd stage larvae showed 100%effectiveness after 24 *** aqueous extract at 10% had a relative growth index of *** the ethanolic and methanolic extract obtained values of 0.76 and 0.70 and control reached *** treated with 10% of methanol,ethanol and aqueous extract showed a reduction in larval duration of 5.00,2.20 and 4.35 days;ethanol extract at 1% provoke decrease of 2.40 days in the development and exhibited an increment of 3.30 days when treated with 0.01%.Aqueous,ethanol and methanol extracts at 10%reduced in 6.15,3.42 and 5.57 days pupal *** main compounds were diallyl disulfide(50.05%),diallyl sulfide(11.77%) and disulfide di-2-propenyl(10.37%).Conclusions:The study demonstrated for the first time,the larvicidal activity of the essential oil and hydrolat of Pseudocalymma alliaceum:aqueous,ethanol and methanol extracts inhibited the normal growth and development of mosquito larvae,prolonging and delaying larval and pupal duration.

关键词： Pseudocallyma Mosquito control growth inhibition Botanical extracts Essential oils Hydrolat

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Sucrose Enhances Tyrosine-induced inhibition of growth in Arabidopsis Seedlings

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Agricultural Science & Technology 2017年第5期18卷 753-755页

作者： Yuting LEI Zhou ZHOU Tiantian ZHI Qi ZHU Chunmei REN Hunan Provincial Key Laboratory of Crop Germplasm Innovation and Utilization College of Bioscience and BiotechnologyHunan Agricultural University Changsha 410128 China College of Horticulture and Landscape Hunan Agricultural University Changsha 410128 China

Tyrosine (Tyr) and sucrose (Suc) play an important role in plant ***,the crosstalk between Tyr and Suc in regulating the plant growth has remained *** this study,it was found that Suc is able to enhance Tyr-induced inhibition of growth in Arabidopsis *** fresh weight and root length of Arabidopsis seedlings were significantly reduced when the medium was added with high concentrations (such as 0.5 or 1.0 mM)of Tyr whereas they were clearly increased with an addition of appropriate concentrations of Suc (such as 1 or 3%) in the medium,which suggested that the growth of Arabidopsis seedlings is inhibited by high concentrations of Tyr but promoted by appropriate concentrations of ***,the inhibition of growth in Arabidopsis seedlings by 0.5 or 1.0 mM Tyr was significantly intensified once 1%or 3%of Suc was added in the *** study revealed a positive effect of Suc on the Tyr-induced inhibition of plant growth,and the results help us to further investigate the crosstalk of Suc and Tyr in the regulation of plant growth.

关键词： Arabidopsis growth inhibition Sucrose Tyrosine

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Toxicity evaluation of ZnO and TiO_2 nanomaterials in hydroponic red bean(Vigna angularis) plant:Physiology, biochemistry and kinetic transport

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Journal of Environmental Sciences 2018年第10期30卷 140-152页

作者： Shanaz Jahan Yatimah Binti Alias Ahmad Farid Bin Abu Bakar Ismail Bin Yusoff Department of Geology Environmental and Earth Sciences Faculty of Science University of Malaya Department of Chemistry Faculty of Science University of Malaya University Malaya Centre for Ionic Liquids (UMCi L) University of Malaya

The toxicity and kinetic uptake potential of zinc oxide（Zn O） and titanium dioxide（TiO2）nanomaterials into the red bean（Vigna angularis） plant were investigated. The results obtained revealed that Zn O, due to its high dissolution and strong binding capacity, readily accumulated in the root tissues and significantly inhibited the physiological activity of the plant. However, TiO2 had a positive effect on plant physiology, resulting in promoted growth. The results of biochemical experiments implied that Zn O, through the generation of oxidative stress, significantly reduced the chlorophyll content, carotenoids and activity of stress-controlling enzymes. On the contrary, no negative biochemical impact was observed in plants treated with TiO2. For the kinetic uptake and transport study, we designed two exposure systems in which Zn O and TiO2 were exposed to red bean seedlings individually or in a mixture approach. The results showed that in single metal oxide treatments, the uptake and transport increased with increasing exposure period from one week to three ***, in the metal oxide co-exposure treatment, due to complexation and competition among the particles, the uptake and transport were remarkably decreased. This suggested that the kinetic transport pattern of the metal oxide mixtures varied compared to those of its individual constituents.

关键词： Metal oxides Physiological impacts Biochemical impacts Uptake kinetics Translocation growth inhibition

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Effects of chitosan-RE^(3+)-bentonite on growth of Chlorella vulgaris

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Journal of Rare Earths 2010年第S1期28卷 149-153页

作者：汪东风李海燕王丽媛张莉徐莹 College of Food Science and Engineering Ocean University of China

Bentonite was modified with chitosan and REIs(La3+,Ce3+) to form chitosan-RE3+-bentonite(CRB) . CRB were characterized by DSC and FT-IR. And the inhibitory effects of CRB on the growth of Chlorella vulgaris were investigated. The results indicated that CRB could inhibit the growth of Chlorella vulgaris. The relative conductivity and the generation of malondialdehyde(MDA) increased. In addition,the superoxide dismutase(SOD) and peroxidase(POD) activities decreased. So CRB could be a promising algaecide agent.

关键词： chitosan bentonite Chlorella vulgaris growth inhibition rare earths

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