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Obstruction of the formation of granulation tissue leads to delayed wound healing after scald burn injury in mice

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Burns & Trauma 2021年第1期9卷 523-539页

作者： Yunxia Chen Xiaorong Zhang Zhihui Liu Jiacai Yang Cheng Chen Jue Wang Zengjun Yang Lei He Pengcheng Xu Xiaohong Hu Gaoxing Luo Weifeng He State Key Laboratory of Trauma Burn and Combined InjuryInstitute of Burn ResearchSouthwest HospitalThird Military Medical University(Army Medical University)Chongqing 400038China Chongqing Key Laboratory for Disease Proteomics Chongqing 400038China Department of Dermatology Southwest HospitalArmy Military Medical UniversityChongqingChina Department of Osteopathic Medicine Southwest HospitalArmy Military Medical UniversityChongqingChina

Background:Delayed wound healing remains a common but challenging problem in patients with acute or chronic wound following accidental scald burn ***,the systematic and detailed evaluation of the scald burn injury,including second-degree deep scald(SDDS)and thirddegree scald(TDS),is still *** present study aims to analyze the wound-healing speed,the formation of granulation tissue,and the healing quality after cutaneous ***:In order to assess SDDS and TDS,the models of SDDS and TDS were established using a scald instrument in C57BL/6 ***,an excisional wound was administered on the dorsal surface in mice(Cut group).The wound-healing rate was first analyzed at days 0,3,5,7,15 and 27,with the Cut group as a ***,on the full-thickness wounds,hematoxylin and eosin(H&E)staining,Masson staining,Sirius red staining,Victoria blue staining and immunohistochemistry were performed to examine re-epithelialization,the formation of granulation tissue,vascularization,inflammatory infiltration and the healing quality at different time points in the Cut,SDDS and TDS ***:The presented data revealed that the wound-healing rate was higher in the Cut group,when compared with the SDDS and TDS groups.H&E staining showed that re-epithelialization,formation of granulation tissue and inflammatory infiltration were greater in the Cut group,when compared with the SDDS and TDS *** revealed that the number of CD31,vascular endothelial growth factor A,transforming growth factor-βandα-smooth muscle actin reached preferential peak in the Cut group,when compared with other *** addition,Masson staining,Sirius red staining,Victoria blue staining,Gordon-Sweets staining and stress analysis indicated that the ratio of collagen I to III,reticular fibers,failure stress,Young’s modulus and failure length in the SDDS group were similar to those in the normal group,suggesting that healing quality was better in the SDDS

关键词： Burn wound healing Second-degree deep scald Third-degree scald Re-epithelialization granulation tissue Healing quality

TRANSFORMATION OF EFFUSION AND FORMATION OF granulation tissue IN PATHOLOGIC PROCESS OF OTITIS MEDIA WITH EFFUSION

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Journal of Pharmaceutical Analysis 2003年第2期15卷 170-172,188页

作者：韦俊荣张青张全安 Department of Otorhinolaryngology Second Hospital of Xi'an Jiaotong University

Objective To explore the diversity of stagnant effusions and the pathologic processes leading to granulation tissue formation in otitis media with effusion(OME). Methods Temporal bone slides from 306 ears with OME were studied histopathologically under the light microscope. Results Results of this study revealed a pathologic process in witch the type and the condition of stagnant effusion in the middle ear cleft was a variable in the dynamics of OME progression from an early stage to an advanced stage. The location of granulation tissue and retentive effusion were found to be closely related. Conclusion Early stage granulation tissue formation exhibited a pathologic process in which granulation tissue formation occurred only in areas where effusion had stagnated or was absorbed. The incidence of the retentive effusion and formation of granulation tissue was much higher and the pathologic changes most extensive in the area around the ossicular chain.

关键词： transformation formation effusion granulation tissue otitis media

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Effects of platelet-rich plasma on in vitro proliferation and migration of fibroblasts from human chronic refractory wound granulation tissue

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Discussion of Clinical Cases 2021年第1期8卷 24-28页

作者： Tie-ning Zhang Quan Li Te Ba Tian-xi Shao Fang Li Ling-feng Wang Baotou Medical College of Inner Mongolia University of Science and Technology BaotouInner MongoliaChina Department of Burns Baogang HospitalBaotouInner MongoliaChina

Objective:To observe the effects of platelet-rich plasma(PRP)on in vitro proliferation and migration of fibroblasts from human chronic refractory wound granulation ***:Fibroblasts were separated from human chronic refractory wound granulation tissue and then were *** obtained fibroblasts were divided into fetal bovine serum(FBS)group,hydrogel group and PRP group,and the three groups were cultured with culture mediums containing FBS,hydrogel and PRP respectively,in order to observe the growth of *** wound scratch assay was used to observe the migration of ***:PRP group had more fibroblasts than FBS group and hydrogel group since Day 5 of culture,and exhibited greater fibroblast scratch migration area than FBS group on 48 h and 72 h of wound scratch assay(all p<.05).Conclusions:Compared with FBS,human fibroblasts cultured by PRP can more effectively promote the proliferation and migration of fibroblasts.

关键词： Fibroblast Chronic refractory wound granulation tissue Platelet-rich plasma Cell proliferation Cell migration In vitro

Effect of three interventional bronchoscopic methods on tracheal stenosis and the formation of granulation tissues in dogs

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Chinese Medical Journal 2010年第5期123卷 621-627页

作者： ZHANG Jie WANG Ting WANG Juan PEI Ying-hua XU Min WANG Yu-ling ZHANG Xia WANG Chen Department of Pulmonary and Critical Care Medicine Beijing Chao-Yang Hospital Capital Medical University Beijing 100020 China Department of Pulmonary Medicine Beijing Tian Tan Hospital Capital Medical University Beijing 100050 China

Background Therapeutic approaches for tracheal stenosis caused by the formation of exuberant granulation tissues usually include electrocautery, mechanical dilation, laser therapy, argon plasma coagulation （APC）, cryotherapy and stent placement. However, restenosis after stent insertion remains a significant limitation. We examined the efficacy of three different approaches, including induction of mechanical lesions, APC performed with different powers or durations and cryotherapy, to identify the method that limited the formation of granulation tissue. Methods Twelve specially bred research mongrel dogs were divided into three groups. In group 1 （four dogs） mild （procedure 1; two dogs） or moderate （procedure 2; two dogs） damage was induced mechanically. Group 2 （six dogs） received APC at different powers or durations （procedure 3：30 W, 1 cm/s; procedure 4：30 W, 2 cm/s; procedure 5：25 W, 3 cm/s）. Group 3 （two dogs） received cryotherapy （procedure 6： two freeze-thaw cycles of 30 seconds）. Uncovered self-expandable metallic stents were inserted in all dogs to maintain a continuous stimulus to the trachea mucosa. Dogs were monitored for 4 weeks and the relationship between granulation tissue proliferation and method used was analyzed. Results In group 1, granulation tissue growth increased with more severe mechanical damage. The growth of granulation tissue in group 2 was more pronounced than in group 1, and both dogs in procedure 3 died because of severe stenosis. In this group, the formation of granulation tissue decreased with decreasing power and duration. In group 3, no obvious granulation tissue was found at week 4. Conclusions Lesions and stimuli from a foreign body （the stent） are two important factors that lead to overgrowth of granulation tissue. Thermal lesions, such as APC, seem to induce greater granulation tissue growth and cartilage damage compared with mechanical and cryotherapy lesions. Cryotherapy in combination with mechanical dilation may be a safe

关键词： interventional bronchoscopy tracheal stenosis granulation tissue

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Preparation and characterization of paclitaxel-loaded poly lactic acid-co-glycolic acid coating tracheal stent

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Chinese Medical Journal 2014年第12期127卷 2236-2240页

作者： Kong Yingying Zhang Jie Wang Ting Qiu Xiaojian Wang Yuling Department of Pulmonary Medicine Beijing Tian Tan HospitalCapital Medical UniversityBeijing 100050China

Background In-stent restenosis caused by airway granulation poses a challenge due to the high incidence of recurrence after *** applications of anti-proliferative drugs have potential value in delaying the recurrence of airway ***,it is not practical to subject patients to repeated bronchoscopy and topical drug *** fabricated novel pacilitaxel-eluting tracheal stents with sustained and slow pacilitaxel release,which could inhibit the formation of granulation *** we assessed the quality and drug release behaviors of drug-eluting stents （DESs） in *** Stents were dipped vertically into a coating solution prepared by dissolving 0.5 g （2％ w/v） of poly lactic acid-coglycolic acid （PLGA） and 0.025 g （0.1％ w/v） of pacilitaxel in 25 ml of *** morphology was examined by scanning electron microscopy （SEM）.Pacilitaxel release kinetics from these DESs was investigated in vitro by shaking in PBS buffer followed by high performance liquid chromatography （HPLC）.Results Using an orthogonal experimental design,we fabricated numerous pacilitaxel/PLGA eluting tracheal stents to assess optimum coating *** optimum coating proportion was 0.1％（w/v） pacilitaxel and 2％（w/v） PLGA,which resulted in total pacilitaxel loading of （16.380 6±0.002 1） mg/*** SEM the coating was very smooth and *** released from DES was at （0.376 3±0.003 8） mg/d,which is a therapeutic *** was a prolonged,sustained release of pacilitaxel of ＞40 *** Paclitaxel-loaded PLGA coating tracheal stents were successfully developed and *** assessments demonstrated favorable surface morphology as well as sustained and effective drug release behavior,which provides an experimental reference for clinical practitioners.

关键词： in-stent restenosis paclitaxel-eluting tracheal stent PLGA slow-release granulation tissue

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高龄患者气管置管并发肉芽组织增生的护理2例

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中国实用护理杂志 2007年第6期23卷 34-35页

作者：闫有梅中国医学科学院中国协和医科大学北京协和医院特需医疗部

气管切开术是抢救呼吸道梗阻患者的一种有效的措施,而人工气道的阻塞问题已成为当今呼吸治疗和危重治疗中的一个重要课题。近年来国内外护理同行在此方面进行了大量的研究[1]。肉芽组织增生是气管切开的后期并发症,它可导

关键词：高龄患者气管置管肉芽组织增生护理 granulation tissue 气管切开术气管套管现报道如下呼吸道梗阻后期并发症阻塞呼吸治疗人工气道临床经验致死抢救课题金属国内功效

Role of angiogenesis and angiogenic factors in acute and chronic wound healing

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Plastic and Aesthetic Research 2015年第1期2卷 243-249页

作者： Thittamaranahalli Muguregowda Honnegowda Pramod Kumar Echalasara Govindarama Padmanabha Udupa Sudesh Kumar Udaya Kumar Pragna Rao Department of Plastic Surgery and Burns Kasturba Medical CollegeManipal 576104KarnatakaIndia Department of Plastic Surgery King Abdulaziz Specialist HospitalSakaka 42421Al-JoufSaudi Arabia Department of Biochemistry Kasturba Medical CollegeManipal 576104KarnatakaIndia Department of Surgery District Government HospitalUdupi 576108KarnatakaIndia

Angiogenesis plays a crucial role in wound healing by forming new blood vessels from preexisting vessels by invading the wound clot and organizing into a microvascular network throughout the granulation *** dynamic process is highly regulated by signals from both serum and the surrounding extracellular matrix *** endothelial growth factor,angiopoietin,fibroblast growth factor and transforming growth factor-beta are among the potent angiogenic cytokines in wound *** endothelial cell ECM receptors are critical for morphogenetic changes in blood vessels during wound *** particular integrin(αvβ3)receptors for fibrin and fibronectin,appear to be required for wound angiogenesis:αvβ3 is focally expressed at the tips of angiogenic capillary sprouts invading the wound clot,and any functional inhibitors ofαvβ3 such as monoclonal antibodies,cyclic RGD peptide antagonists,and peptidomimetics rapidly inhibit granulation tissue *** spite of clear knowledge about influence of many angiogenic factors on wound healing,little progress has been made in defining the source of these factors,the regulatory events involved in wound angiogenesis and in the clinical use of angiogenic stimulants to promote repair.

关键词： Angiogenic factors endothelium extracellular matrix protein granulation tissue wound healing

Comparative Evaluation of Different Treatment for Purulent Wounds in Dogs

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Open Journal of Veterinary Medicine 2016年第7期6卷 119-126页

作者： Ahmad Massoud Niazi Mohammad Monir Tawfeeq Amanullah Aziz Jahid Zabuli Shahpoor Rahmati Abdul Razaq Irshad Clinic Department Faculty of Veterinary Science Kabul University Kabul Afghanistan

To compare the healing of purulent wounds with Shilajit dressing vs. Vishnevsky dressing in dogs with purulent wounds of >2 weeks of duration, eight dogs were chosen from same ages in two groups i.e., Shilajit and Vishnevsky dressing group. Dressing was done on every day basis for more than two weeks of follow up period. Main outcome of healing measure was completed at three weeks. Wound healing status was assessed at three days intervals till end of three weeks. Shilajit treated achieved complete decrease in the wound surface area, effect of healing score in Shilajit dressing group in comparison to the Vishnevsky dressing group at p > 0.05 level of significance. Shilajit is highly effective in achieving a characteristic feature of regenerative and granulation healing of purulent wounds as compared to Vishnevsky dressing group in dogs.

关键词： Shilajit Wound Dressing Purulent Wound of Dog granulation tissue

Histopathological study of chronic wounds modulated by intermittent negative pressure therapy under limited access dressing

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Plastic and Aesthetic Research 2015年第1期2卷 272-276页

作者： Thittamaranahalli Muguregowda Honnegowda Pramod Kumar Rekha Singh Swarna Shivakumar Pragna Rao Hemanth K.Prasad Sudesh Kumar Udaya Kumar Echalasara Govindarama Padmanabha Udupa Department of Plastic Surgery and Burns Kasturba Medical CollegeManipal 576104KarnatakaIndia Department of Plastic Surgery King Abdulaziz Specialist HospitalSakaka 42421Al-JoufSaudi Arabia Department of Pathology Vydehi Institute of Medical Sciences and Research CenterBengaluru 560066KarnatakaIndia Department of Pathology Apollo HospitalsBengaluru 560066KarnatakaIndia Department of Biochemistry Kasturba Medical CollegeManipal 576104KarnatakaIndia Department of Surgery Government District HospitalUdupi 576101KarnatakaIndia

Aim:Negative pressure wound therapy(NPWT)has achieved widespread success in the treatment of chronic ***,its effects have been only partially explored,and investigations have generally concentrated on the wound-dressing interface;a detailed histopathological description of the evolution of wounds under NPWT is still *** present study was performed to investigate the effect of a limited access dressing(LAD)which exerts intermittent NPWT in a moist environment on chronic ***:A total of 140 patients were randomized into 2 groups:LAD group(n=64)and conventional dressing group(n=76).By histopathological analysis of the granulation tissue,the amount of inflammatory infiltrate,necrotic tissue,angiogenesis,and extracellular matrix(ECM)deposition was studied and compared to determine healing between the 2 ***:After 10 days of treatment,histopathological analysis showed a significant decrease in necrotic tissue with LAD compared to the conventional dressing group(mean±standard error,11.5±0.48 vs.10.1±0.30,P=0.007),the number of inflammatory cells(12.6±0.60 vs.8.63±0.35,P=0.018),a significant increase in new blood vessels(12.8±0.58 vs.9.3±0.29,P=0.005)and ECM deposit(13.3±0.50 vs.9.6±0.24,P=0.001).Conclusion:LAD exerts its beneficial effects on chronic wound healing by decreasing the amount of necrotic tissue and inflammatory cells while increasing the amount of ECM deposition and angiogenesis.

关键词： Angiogenesis chronic wounds extracellular matrix granulation tissue inflammatory cell limited access dressing necrotic tissue negative pressure wound therapy