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Chinese Chemical Letters 2022年第3期33卷 1619-1622页

作者： Kefurong Deng Yachao Li Xiaoyu Liang Cheng Shen Zenan Zeng Xianghui Xu Department of Pharmacy College of BiologyHunan UniversityChangsha 410082China

Infectious diseases become one of the leading causes of human death. Traditional treatment based on classical antibiotics could not provide enough antibacterial activity to combat bacterial infections due to low bioavailability, even leading to antibiotic resistance. In recent years, biomimetic delivery systems have been developed to improve drug therapy for various diseases, such as malignant tumor and cardiovascular disease. In this work, we designed virus-inspired nanodrugs(VNDs) through co-assembly of amphiphilic lipopeptide dendrons and poly(lactic-co-glycolic acid) polymers for high-efficiency antibiotic delivery. These VNDs had well-defined and stable nanostructures for tetracycline encapsulation and delivery. The VNDs were capable of promoting antibiotic internalization and enhancing their antibacterial effects against gram-negative Escherichia coli and gram-positive Staphylococcus aureus. Additionally, no obvious cytotoxicity of VNDs was observed to human cell lines. This work successfully demonstrated the virus-mimetic nanoparticles served as promising and applicable antibiotic delivery platform for antibacterial treatment.

关键词： Virus-inspired nanoparticles bacterial intracellular drug delivery Antibacterial treatment gram-positive bacteria gram-negative bacteria

Selective inactivation of gram-positive bacteria in vitro and in vivo through metabolic labelling

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Science China Materials 2022年第1期65卷 237-245页

作者： Tao Feng Hui Lu Xiaoting Ye Chaofan Nie Jianhong Zhang Luofeng Yu Haoyu Jin Peng Li Wei Huang Frontiers Science Center for Flexible Electronics(FSCFE) Xi’an Institute of Flexible Electronics(IFE)Xi’an Institute of Biomedical Materials and Engineering(IBME)Ningbo Institute&Chongqing Technology Innovation CenterNorthwestern Polytechnical University(NPU)Xi’an 710072China Key Laboratory of Flexible Electronics(KLOFE)&Institute of Advanced Materials(IAM) Nanjing Tech University(NanjingTech)Nanjing 211816China Key Laboratory for Organic Electronics and Information Displays&Institute of Advanced Materials(IAM) Nanjing University of Posts and TelecommunicationsNanjing 210023China

bacterial infections are grave threats to human health,particularly those caused by the most common grampositive *** massive administration of broad-spectrum antibiotics to treat various bacterial infections has led to the evolution and spread of drug *** a universal antimicrobial technique unapt to induce drug resistance,photothermal therapy(PTT)is attracting extensive attention in recent ***,its unspecific killing capability and side effects towards adjacent mammalian cells severely impede the practical ***,we proposed a metabolic engineering strategy to selectively inactivate gram-positive bacteria by PTT.A bioorthogonal photothermal agent was prepared by the conjugation of IR-780 iodide and dibenzocyclooctyne(IR780-DBCO).Upon pre-metabolizing with 3-azido-D-alanine,gram-positive bacteria rather than gramnegative ones,such as Staphylococcus aureus and vancomycinresistant Enterococcus faecalis(VRE),could be specifically tied up by the explosive IR780-DBCO via copper-free click ***,they spontaneously detonated under 15 min near-infrared light irradiation and inactivated nearly 100% gram-positive bacteria in ***,superbug VRE-induced infection was significantly inhibited by this approach in a mouse skin wound *** metabolic labelling-based photothermal ablation strategy specific to gram-positive microbes would stimulate the development of precise antibacterial candidates for preclinical applications.

关键词： gram-positive bacteria selective inactivation photothermal agent metabolic labelling wound infection

Specific discrimination of gram-positive bacteria and direct visualization of its infection towards mammalian cells by a DPAN-based AIEgen

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Specific discrimination of gram-positive bacteria and direct...

Antibacterial activity of bioceramic coatings on Mg and its alloys created by plasma electrolytic oxidation(PEO): A review

第十一届全国化学生物学学术会议

作者： Ping He Rong Hu Fan Zhou Taotao Zhou Jianlei Shen Zhiming Wang Zujin Zhao Anjun Qin Ben Zhong Tang State Key Laboratory of Luminescent Materials and Devices Center for Aggregation-induced EmissionGuangzhou International campusSouth China University of Technology HKUST-Shenzhen Research Institute Department of Chemistry Hong Kong Branch of Chinese National Engineering Research Centre for Tissue Restoration and ReconstructionInstitute for Advanced Studyand Department of Chemical and Biological EngineeringThe Hong Kong University of Science ＆ Technology （HKUST）

The infections caused by gram-positive bacteria（G+） is a major threat to public health due to their high morbidity and mortality, and the identification of G+ with high sensitivity and selectivity is urgently desirable. In this paper, a probe with aggregation-induced emission（AIE） characteristic is developed and used to discriminate G+ from other bacteria and fungus with excellent selectivity. Thanks for its AIE feature, this probe shows intense fluorescence when interacted with G+, and the signal can last for 24 h after incubation with S. aureus, which is benefit for long term tracing of translocation process with high contrast. Moreover, this probe can be used to visualize the infection processes of G+ towards mammalian cells, which provides valuable information for understanding the therapeutic mechanism.

关键词： aggregation-induced emission gram-positive bacteria specificity discrimination visualization

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Journal of Magnesium and Alloys 2022年第1期10卷 88-106页

作者： Arash Fattah-alhosseini Maryam Molaei Meisam Nouri Kazem Babaei Department of Materials Engineering Bu-Ali Sina UniversityHamedan 65178-38695Iran

Mg and its alloys are suitable choices for implant materials due to their biodegradability and biocompatibility features. However, the high electrochemical activity of this family of biomaterials results in their fast degradation and severe corrosion in the physiological environment,producing hydrogen(H;) gas, and therefore increasing the p H of the environment. To meet the clinical requirements, the degradation rate of Mg biomaterials needs to be reduced. Nevertheless, higher corrosion resistance of Mg results in a low alkaline p H, weakening the antibacterial activity. Therefore, while the rapid degradation problem of Mg-based biomaterials needs to be addressed, good antibacterial properties are also necessary. By using the plasma electrolytic oxidation(PEO) surface modification technique, the antibacterial activity of Mg and its alloys can be enhanced while maintaining their corrosion protection properties at a high level. Throughout the PEO process, introducing antibacterial agents into solutions results in a major increase in antibacterial activity of the coatings. Moreover, post-or pre-processing on PEO coatings can provide better protection against bacteria. In this review, the antibacterial activity of PEO coatings applied on Mg and also its alloys will be discussed in more detail.

关键词： Antibacterial activity Magnesium Plasma electrolytic oxidation(PEO) gram-positive bacteria gram-negative bacteria

Highly fluorescent,photostable,and biocompatible silicon theranostic nanoprobes against Staphylococcus aureus infections

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Nano Research 2018年第12期11卷 6417-6427页

作者： Xia Zhai Bin Song Binbin Chu Yuanyuan Su Houyu Wang Yao He Laboratory of Nanoscale Biochemical Analysis lnstitute of Functional Nano &Soft Materials (FUNSOM)and Collaborative Innovation Center of Suzhou Nano Science and Technology (NANO-CIC)Soochow UniversitySuzhou 215123China

Human health is severely threatened by bacterial infections, which fuel urgent demand for the development of novel theranostic agents that are suitable for diagnosing and combating bacterial infections. To meet this goal, we synthesized a new kind of theranostic probes, made of vancomycin (Van)-modified fluorescent silicon nanoparticles (SiNPs-Van). The new probes feature good selectivity for gram-positive bacterial infections, favorable fluorescence, strong resistance to photobleaching (e.g., 12% loss of fluorescence intensity during 40 min continuous laser excitation in the presence of Staphylococcus aureus (S. aureus)), and good biocompatibility (e.g., cell viability > 95% during 24 h treatment). Because of these benefits, the probes were effective at prolonged (i.e., 8-day) fluorescence tracking of S. aureus infections in vivo, thus providing a visible and accurate way to evaluate treatment efficacy. Moreover, the resultant SiNPs-Van with a minimum inhibitory concentration (MIC) of ca. 0.5 μg/mL exhibited high antibacterial efficiency of 92.5%, superior to that (76.5%) of free Van with higher MIC of ca. 1 μg/mL.

关键词： fluorescent silicon nanoparticles gram-positive bacteria Staphylococcus aureus prolonged extended period

Comparative study between three carbonaceous nanoblades and nanodarts for antimicrobial applications

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Journal of Environmental Sciences 2024年第2期136卷 594-605页

作者： Mohamed S.Selim Ahmed M.Azzam Mohamed A.Shenashen Shimaa A.Higazy Bayaumy B.Mostafa Sherif A.El-Safty National Institute for Materials Science(NIMS) 1-2-1 SengenTsukuba-ShiIbaraki-Ken 305-0047Japan Petroleum Application Department Egyptian Petroleum Research InstituteNasr City 11727Egypt Department of Environmental Research Theodor Bilharz Research InstituteGizaEgypt

The design of nanostructured materials occupies a privileged position in the development and management of affordable and effective technology in the antibacterial ***,we discuss the antimicrobial properties of three carbonaceous nanoblades and nanodarts materials of graphene oxide(GO),reduced graphene oxide(RGO),and single-wall carbon nanotubes(SWCNTs)that have a mechano-bactericidal effect,and the ability to piercing or slicing bacterial *** demonstrate the significance of size,morphology and composition on the antibacterial activity mechanism,the designed nanomaterials have been *** minimum inhibitory concentration(MIC),standard agar well diffusion,and transmission electron microscopy were utilized to evaluate the antibacterial activity of GO,RGO,and *** on the evidence obtained,the three carbonaceous materials exhibit activity against all microbial strains tested by completely encapsulating bacterial cells and causing morphological disruption by degrading the microbial cell membrane in the order of RGO>GO>*** of the external cell wall structure and outer membrane proteins,the synthesized carbonaceous nanomaterials exhibited higher antibacterial activity against gram-positive bacterial strains than gram-negative and fungal *** had the lowest MIC values(0.062,0.125,and 0.25 mg/mL against ***,***,and ***,respectively),as well as minimum fungal concentrations(0.5 mg/mL for both *** and ***).At 12 hr,the cell viability values against tested microbial strains were completely *** lysis and death occurred as a result of severe membrane damage caused by microorganisms perched on RGO *** work gives an insight into the design of effective graphene-based antimicrobial materials for water treatment and remediation.

关键词： Antimicrobial properties Nanoblades Mechano-bactericidal effect RGO nanosheets gram-positive bacteria Cell viability

High pyrolysis temperature biochar reduced the transport of petroleum degradation bacteria Corynebacterium variabile HRJ4 in porous media

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Journal of Environmental Sciences 2021年第2期33卷 228-239页

作者： Saisai Guo Xiaomei Liu Hang Zhao Lan Wang Jingchun Tang College of Environmental Science and Engineering Nankai UniversityTianjin 300350China Key Laboratory of Pollution Processes and Environmental Criteria(Ministry of Education) Tianijin 300350China Tianjin Engineering Research Center of Enuironmental Diagnosis and Contamination Remediation Tianjin 300350China

Biochar has been widely applied for the remediation of petroleum-contaminated ***,the effect of biochar on the transport of petroleum degradation bacteria has not been studied.A typical gram-positive petroleum degradation bacteria-Corynebacterium variabile HRJ4 was used to study the effect of different biochars on bacterial transport and *** indicated that the addition of biochar in sand was effective for reducing the transport of bacteria and poplar sawdust biochar(PSBC)had a stronger hinder effect than corn straw biochar(CSBC).The hindrance was more evident with pyrolysis temperature of biochar raised from 300℃ to 600℃,which was attributed to the increase of specific surface area(309 times).The hindrance effect also enhanced with higher application rate of ***,the reduction of HRJ4 transport was more obvious in higher(25 mmol/L)concentration of Na Cl solution owing to electrostatic attraction *** adsorption of biochar to HRJ4 was defined to contribute to the hindrance of HRJ4 transport *** the influence of feedstocks and pyrolysis temperature on HRJ4 transport,it suggested that specific surface area had the greatest effect on HRJ4 transport,and pore-filling,electrostatic force also contributed to HRJ4 retained in quartz sand *** last,phenol transportation experiment indicated that the restriction of biochar on HRJ4 enhanced the phenol removal rate in the *** study provides a theoretical basis for the interaction of biochar and bacteria,which is vital for the remediation of oil-contaminated soil and groundwater in the field.

关键词： gram-positive bacteria Feedstocks HRJ4 Transport Retention

Accumulation of Rare Earth Elements in Various Microorganisms

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Journal of Rare Earths 2007年第5期25卷 526-532页

作者： Takehiko Tsuruta Department of Chemical Engineering on Biological Environment Hachinohe Institute of Technology 88-1 Azaohbiraki Onazamyoh Hachinohe Aomori 031-8501 Japan

The removal of rare earth elements （REEs） from solution in various microorganisms was examined. Seventy-six strains from 69 species （22 bacteria, 20 actinomycetes, 18 fungi, and 16 yeasts） were tested. Initially, Sm was used to test the removal capabilities of the various organisms. gram-positive bacteria, such as Bacillus licheniformis, B. subtilis, Brevibacterium helovolum, and Rhodococcus elythropolis, exhibited a particularly high capacity for accumulating Sm. In particular, the B. lichemiformis cells accumulated approximately 316 μmol Sm per gram dry wt. of microbial cells. A full suite of screenings was then conducted to compare the abilities of the organisms to remove Se, Y, La, Er, and, Lu from solution. Tests were done with solutions containing one REE at a time. Accumulation was nearly identical for the various metals and organisms. However, when solutions with equimolar amounts of two REEs were used, preferential removal from solution was observed. When an Eu/Gd solution was used, gram-positive bacteria removed more Eu and Gd as compared to actinomycetes. When Eu/Sm combination was used, gram-positive bacteria removed equal mounts of both metals and some actinomycetes removed more Eu. The selective removal was quantified by calculating separation factors （S. F.）, which indicated that Streptomyces levoris cells accumulated the greatest proportion of Eu. The removal of REEs from a solution containing five metals （Y, La, Sm, Er, and Lu） was then examined. Mucorjavanicus preferentially accumulated Sm and S. flavoviridis preferentially accumulated Lu. The effects of pH and Sm concentration on the accumulation of Sm by B. licheniformis were also examined. Accumulation increased at higher pH and at greater solution concentrations.

关键词： Sm accumulation selective rare earth accumulation microorganism gram-positive bacteria actinomycetes

A widespread response of gram-negative bacterial acyl-homoserine lactone receptors to gram-positive Streptomyces γ-butyrolactone signaling molecules

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Science China(Life Sciences) 2021年第10期64卷 1575-1589页

作者： Xiang Liu Wenxi Wang Junyue Li Yue Li Jihui Zhang Huarong Tan State Key Laboratory of Microbial Resources Institute of MicrobiologyChinese Academy of SciencesBeijing 100101China College of Life Sciences University of Chinese Academy of SciencesBeijing 100049China

Cell-cell communication is critical for bacterial survival in natural habitats,in which miscellaneous regulatory networks are ***,elucidating the interaction networks of a microbial community has been hindered by the population *** study reveals thatγ-butyrolactone(GBL)molecules from Streptomyces species,the major antibiotic producers,can directly bind to the acyl-homoserine lactone(AHL)receptor of Chromobacterium violaceum and influence violacein production controlled by the quorum sensing(QS)***,the widespread responses of more gram-negative bacterial AHL receptors to gram-positive Streptomyces signaling molecules are *** on the cross-talk between GBL and AHL signaling systems,combinatorial regulatory circuits(CRC)are designed and proved to be workable in Escherichia coli(***).It is significant that the QS systems of gram-positive and gram-negative bacteria can be bridged via native Streptomyces signaling *** findings pave a new path for unlocking the comprehensive cell-cell communications in microbial communities and facilitate the exploitation of innovative regulatory elements for synthetic biology.

关键词： signaling communication cross-talk gram-positive bacteria Streptomyces γ-butyrolactone gram-negative bacteria acyl-homoserine lactone synthetic biology combinatorial regulatory circuit

Causative Microorganisms Isolated from Patients with Intra-Abdominal Infections and Their Drug Resistance Profiles:An 11-Year(2011–2021)Single-Center Retrospective Study

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Biomedical and Environmental Sciences 2023年第8期36卷 732-742页

作者： DING Rui MA Rui Rui LIU Ya Li ZHAO Ying GUO Li Na DOU Hong Tao SUN Hong Li LIU Wen Jing ZHANG Li WANG Yao LI Ding Ding YI Qiao Lian XU Ying Chun Department of Laboratory Medicine State Key Laboratory of Complex Severe and Rare DiseasesPeking Union Medical College HospitalChinese Academy of Medical Science and Peking Union Medical CollegeBeijing 100730China Beijing Key Laboratory for Mechanisms Research and Precision Diagnosis of Invasive Fungal Diseases Beijing 100730China Graduate School Chinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100730China

Objective To investigate the distribution and antimicrobial susceptibility of causative microorganisms recovered from patients with intra-abdominal infections(IAIs).Methods A total of 2,926 bacterial and fungal strains were identified in samples collected from 1,679 patients with IAIs at the Peking Union Medical College Hospital between 2011 and *** bacteria and fungi were identified using matrix-assisted laser desorption/ionization time-of-flight mass *** susceptibility testing(AST)was performed using the VITEK 2 compact system and the Kirby–Bauer *** results were interpreted based on the M100-Ed31 clinical breakpoints of the Clinical and Laboratory Standards *** Of the 2,926 strains identified,49.2%,40.8%,and 9.5%were gram-negative bacteria,gram-positive bacteria,and fungi,*** coli was the most prevalent pathogen in intensive care unit(ICU)and non-ICU patients;however,a significant decrease was observed in the isolation of *** between 2011 and ***,significant decreases were observed between 2011 and 2021 in the levels of extended-spectrumβ-lactamase(ESBL)-producing ***(from 76.9%to 14.3%)and Klebsiella pneumoniae(from 45.8%to 4.8%).Polymicrobial infections,particularly those involving co-infection with gram-positive and gram-negative bacteria,were commonly observed in IAI ***,Candida albicans was more commonly isolated from hospital-associated IAI samples,while Staphylococcus epidermidis had a higher ratio in community-associated ***,AST results revealed that most antimicrobial agents performed better in non-ESBL-producers than in ESBL-producers,while the overall resistance rates(56.9%–76.8%)of Acinetobacter baumanmii were higher against all antimicrobial agents than those of other common gram-negative ***,Enterococcus faecium,Enterococcus faecalis,***,and *** were consistently found to be susceptible to vancomycin

关键词： Intra-abdominal infection Causative microorganisms Antimicrobial susceptibility testing gram-negative bacteria gram-positive bacteria