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Isolation and characterization of a sterile-dwarf mutant in Asian cotton (gossypium arboreum L.)

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Journal of Genetics and Genomics 2009年第6期36卷 343-353页

作者： Chuntai Wu Baoliang Zhou Tianzhen Zhang National Key Laboratory of Crop Genetics and Germplasm Enhancement Cotton Research Institute Nanjing Agricultural University Nanjing 210095 China

Plant height is an important trait in cotton. To elucidate the molecular mechanisms of the dwarf phenotype, a sterile-dwarf mutant derived from gossypium arboreum L. cv. Jinhuazhongmian was developed by ^60Co y-ray irradiation. The results demonstrated that the steriledwarf mutant phenotype was controlled by a pair of recessive gene, which was designated sd^a. Plants carrying the sd^a gene contained lower levels of indole-3-acetic acid （IAA） and abscisic acid （ABA） compared with wild-type （WT） plants. The chlorophyll content and net photosynthetic rate in mutant leaves were markedly decreased. However, it was possible that ABA biosynthesis or signaling was involved in governing the sd^a phenotype. Semi-quantitative RT-PCR analysis detected 13 differentially expressed ESTs, and the steriledwarf mutant exhibited decreased expression levels relative to the WT. The role of nine potential hormone biosynthetic genes in the synthesis of IAA, ABA, polyamines （PAs） and jasmonic acid （JA） were discussed.

关键词： gossypium arboreum sd^a auxin abscisic acid ESTs

Identification and Characterization of Cotton Genes Involved in Fuzz-Fiber Development

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Journal of Integrative Plant Biology 2013年第7期55卷 619-630页

作者： Gaskin Wang Guo-Hong Zhao Yin-Hua Jia Xiong-Ming Du State Key Laboratory of Cotton Biology Institute of Cotton Research of the Chinese Academy of Agricultural Sciences

Cotton fuzz fibers, like Arabidopsistrichomes, are elongated unicells. It is postulated that a transcriptional complex of GLABRA1 （GL1）, GLABRA3 （GL3）, and TRANSPARENT TESTAGLABRA1 （TTG1） might be in existence in Arabidopsis as evidenced by their physical interaction in yeast, and the complex regulates expression of GLABRA2 （GL2） controlling trichome cell differentiation; it is also assumed that TRIPTYCHON （TRY） and CAPRICE （CPC） counteract the complex formation in neighboring cells. Here, the homologs GaMYB23 （a homolog of GL1）, GaDEL65 （a homolog of GL3）, GaTTG1, GaCPC and GaTRY were identified in gossypium arboreum. We show that GaMYB23 can bind to and activate the promoters of GaCPC, GaGL2 and GaTRY, and that GaMYB23, GaTRY and GaTTG1 could interact with GaDEL65 in yeast and in planta. In situ analysis showed that GaMYB23, GaGL2, GaDEL65, and GaTRY were predominantly expressed in fuzz fiber, but GaTRY proteins were primarily found in undeveloped epidermal cells. A G. arboreum fuzzless mutant with consistently high level GaMYB23 transcript has lost the detectable GaMYB23-promoter of GaGL2 complex, corresponding to sharply reduced transcription of GaGL2. Our results support that cotton homologs to the genetic molecules regulating Arabidopsis trichome differentiation interacted in the epidermis of ovules and the redundant GaMYB23 serves as a negative regulator in fuzz-fiber patterning.

关键词： Convergent evolution fuzz, fuzzless mutant gossypium arboreum lateral inhibitor trichome.

Polysaccharide composition during cotton seed fibre development:temporal differences between species and in different seasons

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Journal of Cotton Research 2022年第4期5卷 12-24页

作者： PETTOLINO Filomena A. YULIA Dina BACIC Antony LLEWELLYN Danny J. Commonwealth Scientific and Industrial Research Organisation(CSIRO) Agri-culture and FoodCanberraACT 2601Australia Department of Animal Plant&Soil SciencesLa Trobe Institute for Agriculture and FoodLa Trobe UniversityAgriBio BuildingBundooraVIC 3083Australia

Background:Cotton seed fibres are long single-celled epidermal trichomes that first appear on the surface of the ovule at anthesis and then elongate rapidly over a period of 15-25 days until a secondary cell wall(SCW)begins to develop through a rapid increase in the deposition of microfibrillar cellulose between the plasma membrane and the primary cell wall that eventually terminates *** measurements of the different polysaccharide components in both wall types over time and how they influence fibre quality can direct studies involved in enhanc-ing fibre properties for yarn quality through cell wall manipulation or molecular ***:A detailed chemical analysis of cell wall composition by differential solvent fractionation was used to identify the range of polysaccharides present in mature cotton fibres and used to validate a simpler total cell wall monosaccharide linkage analysis protocol for wall compositional *** of fibres from 5 days post-anthesis through maturity for three cultivated species,gossypium hirsutum,***,and ***,showed the dynamic nature of cell wall polysaccharide composition through fibre development and that it progressed differently for each *** grown in the glasshouse during either autumn to winter or spring to summer and within each species had fibre qualities and temporal aspects of cell wall development that were different for each ***,the timing of the deposition of the SCW was delayed in winter grown plants and appeared to influence key fibre qual-ity ***:These results suggest that the temporal aspects of cell wall polysaccharide biogenesis during fibre development influence final fibre quality,and this timing is determined by both genetic and environmental *** onset of SCW synthesis appears to be a critical factor coinciding with termination of fibre elongation and specify-ing the duration of wall thickening that then affects fibre le

关键词： Fibre development gossypium hirsutum gossypium barbadense gossypium arboreum