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Synaptic development of layer V pyramidal neurons in the prenatal human prefrontal neocortex: a Neurolucida-aided golgi study

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Neural Regeneration Research 2020年第8期15卷 1490-1495页

作者： Li-Xin He Lily Wan Wei Xiang Jian-Ming Li An-Hua Pan Da-Hua Lu Xiangtan Medicine and Health Vocational College XiangtanHunan ProvinceChina Department of Neurology Xiangya HospitalCentral South UniversityChangshaHunan ProvinceChina Changde Vocational Technical College ChangdeHunan ProvinceChina Department of Anatomy Changsha Medical UniversityChangshaHunan ProvinceChina Department of Anatomy and Neurobiology Xiangya School of MedicineCentral South UniversityChangshaHunan ProvinceChina

The prefrontal neocortex is involved in many high cognitive functions in humans.Deficits in neuronal and neurocircuitry development in this part of the cerebrum have been associated with various neuropsychiatric disorders in adolescents and adults.There are currently little available data regarding prenatal dendrite and spine formation on projecting neurons in the human prefrontal neocortex.Previous studies have demonstrated that golgi silver staining can identify neurons in the frontal lobe and visual cortex in human embryos.In the present study,five fetal brains,at 19,20,26,35,and 38 gestational weeks,were obtained via the body donation program at Xiangya School of Medicine,Central South University,China.golgi-stained pyramidal neurons in layer V of Brodmann area 46 in fetuses were quantitatively analyzed using the Neurolucida morphometry system.Results revealed that somal size,total dendritic length,and branching points of these neurons increased from 26 to 38 gestational weeks.There was also a large increase in dendritic spines from 35 to 38 gestational weeks.These findings indicate that,in the human prefrontal neocortex,dendritic growth in layer V pyramidal neurons occurs rapidly during the third trimester of gestation.The use of human fetal brain tissue was approved by the Animal Ethics Committee of Xiangya School of Medicine,Central South University,China(approval No.2011-045)on April 5,2011.

关键词： Golgi staining human brain banking neurodevelopment Neurolucida neuropsychiatric disorders prefrontal cortex synaptogenesis three-dimensional reconstruction

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Acupuncture improves dendritic structure and spatial learning and memory ability of Alzheimer's disease mice

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Neural Regeneration Research 2018年第8期13卷 1390-1395页

作者： Bo-Hong Kan Jian-Chun Yu Lan Zhao Jie Zhao Zhen Li Yan-Rong Suo Jing-Xian Han First Teaching Hospital of Tianjin University of Traditional Chinese Medicine Tianjin China Tianjin Key Laboratory of Acupuncture & Moxibustion Science Tian)in China Institute of Acupuncture and Moxibustion Research Tianjin China Tianjin University of Traditional Chinese Medicine Tianjin China

Acupuncture can improve the cognitive state of Alzheimer＇s disease, but its mechanism is not clear. Dendritic atrophy and synaptic loss in Alzheimer＇s disease brain are positively correlated with cognitive damage. Therefore, we speculated that the effect of acupuncture on improving cognitive function may be associated with reduced dendritic damage in the brain. Acupuncture at Qihai（CV6）, Zhongwan（CV12）, Danzhong（CV17）, bilateral Zusanli（ST36）, and bilateral Xuehai（SP10） acupoints was performed once a day（1-day rest after 6-day treatment） for 14 consecutive days. Senescence-accelerated mouse prone 8（SAMP8） mice without acupuncture and senescence-accelerated mouse resistant 1（SAMR1） mice were used as normal controls. After 14 days of treatment, spatial learning and memory ability of mice was assessed in each group using the Morris water maze. Dendritic changes of pyramidal cells in the hippocampal CA1 region were analyzed by quantitative Golgi staining. Our results showed that acupuncture shortened escape latency and lengthened retention time of the former platform quadrant in SAMP8 mice. Further, SAMP8 mice exhibited a significant increase in the number of apical and basal dendritic branches and total length of apical and basal dendrites after acupuncture. These results suggest that acupuncture improves spatial learning and memory ability of middle-aged SAMP8 mice by ameliorating dendritic structure.Acupuncture can improve the cognitive state of Alzheimer＇s disease, but its mechanism is not clear. Dendritic atrophy and synaptic loss in Alzheimer＇s disease brain are positively correlated with cognitive damage. Therefore, we speculated that the effect of acupuncture on im- proving cognitive function may be associated with reduced dendritic damage in the brain. Acupuncture at Qihai （CV6）, Zhongwan （CV 12）, Danzhong （CV17）, bilateral Zusanli （ST36）, and bilateral Xuehai （SP10） acupoints was performed once a day （1-day rest after 6-day treat- ment） for 14 consecutive days. Senescence-accelerated mouse prone 8 （SAMP8） m

关键词： nerve regeneration Alzheimer＇s disease senescence-accelerated prone mouse 8 acupuncture cognition dendrite hippocampal CA 1 region Morris water maze Golgi staining neural regeneration

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Roles of FGF13 in the regulation of neuronal spine development in mouse brain

Roles of FGF13 in the regulation of neuronal spine developme...

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中国神经科学学会第十届全国学术会议

作者： Xing-Yu Pan Qing-Feng Wu Liu Yang Shuai Li Jing-Rong Zhao Lei Li Lan Bao Xu Zhang Institute of Neuroscience and State Key Laboratory of Neuroscience Shanghai Institutes for Biological SciencesChinese Academy of Sciences State Key Laboratory of Cell Biology Institute of Biochemistry and Cell BiologyShanghai Institutes for Biological SciencesChinese Academy of Sciences

Objective Fibroblast growth factor 13（FGF13）,a non-secretary protein of FGF family,is a candidate gene for X-chromosome-linked mental retardation（XLMR）.We previously found that FGF13 acted intracellularly as a microtubule-stabilizing protein required for axon and leading process development and neuronal migration in the cerebral cortex,and genetic deletion of FGF13 in mice exhibited weakened learning and memory.Here we explore the potential functions of FGF13 in regulating neuronal spine morphology and densities in mouse brain.Methods（1）Knocking down FGF13 with shRNA and examining the dendritic spine morphology and densities of cultured hippocampal neurons.（2） Knocking down FGF13 in embryonic mouse brains with in utero electroporation and examining spine densities of cortical pyramidal neurons.（3） staining neuron morphology with Golgi staining in FGF13-deficient mice and examining the spine densities in several brain regions.Results（1） Basal dendrite spine density was reduced in FGF13-deficient neurons,both in cultured neurons in vitro and in in utero electroporated mice in vivo.（2） Golgi staining of 14-day postnatal mouse brain of FGF13 conditional knock-out lines showed similar decrease in spine density in sensory cortex.Conclusion FGF13 regulates spinogenesis and spine development,and may influence synaptic transmission and maintain mature neuronal networks in mouse brains.

关键词： FGF13 dendritic spine cultured hippocampal neuron in utero electroporation Golgi staining

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