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Single Particle Colorimetric Acid Phosphatase Activity Assay with CeO_(2)-modified gold nanoparticles

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Chemical Research in Chinese Universities 2024年第2期40卷 320-325页

作者： ZHU Tang Jiayao YUAN Xiang MAO Guojiang WEI Lin XIAO Lehui Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research National and Local Joint Engineering Laboratory for New Petrochemical Materials and Fine Utilization of ResourcesCollege of Chemistry and Chemical EngineeringHunan Normal UniversityChangsha410081P.R.China Henan Key Laboratory of Organic Functional Molecule and Drug Innovation Collaborative Innovation Center of Henan Province for Green Manufacturing of Fine ChemicalsKey Laboratory of Green Chemical Media and ReactionsMinistry of EducationSchool of Chemistry and Chemical EngineeringHenan Normal UniversityXinxiang453007P.R.China College of Chemistry and Chemical Engineering Central South UniversityChangsha410083P.R.China

Acid phosphatase(ACP)is a ubiquitous phosphatase in living organisms.The abnormal variation of ACP is related to various diseases.Herein,we propose a colorimetric method based on CeO_(2)-modified gold core shell nanoparticles(Au@CeO_(2)NPs)to analyze ACP activity with high sensitivity and specificity.In this design,2-phospho-L-ascorbic acid trisodium salt(AAP)is dephosphorylated by ACP and produces reductive ascorbic acid(AA),which makes the CeO_(2)shell decomposition.A remarkable blue shift of localized surface plasmon resonance peak(LSPR,from yellow to green)along with the scattering intensity ratio changes from individual Au@CeO_(2)NPs are observed.ACP activity can be quantified by calculating the ratio changes of individual Au@CeO_(2)NPs.This assay reveals limit of detection(LOD)of 0.044 mU/mL and the linear range of 0.05–5.0 mU/mL,which are much lower than most of spectroscopic measurements in bulk solution.Furthermore,the recovery measurements in real samples are satisfactory and the capacity for practical application is demonstrated.As a consequence,Au@CeO_(2)NPs used in this assay will find new applications for the ultrasensitive detection of enzyme activity.

关键词： Single particle imaging Dark field gold nanoparticle Enzyme activity Colorimetric detection

Demonstration of biophoton-driven DNA replication via gold nanoparticle-distance modulated yield oscillation

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Nano Research 2021年第1期14卷 40-45页

作者： Na Li Daoling Peng Xianjing Zhang Yousheng Shu Feng Zhang Lei Jiang Bo Song School of Optical-Electrical Computer Engineering University of Shanghai for Science and TechnologyShanghai 200093China State Key Laboratory of Respiratory Disease Key Laboratory of Oral MedicineGuangzhou Institute of Oral DiseaseStomatology HospitalDepartment of Biomedical EngineeringSchool of Basic Medical SciencesGuangzhou Medical UniversityGuangzhou 511436China School of Chemistry South China Normal UniversityGuangzhou 510006China State Key Laboratory of Medical Neurobiology Institute for Translational Brain ResearchMOE Frontiers Center for Brain ScienceDepartment of NeurologyHuashan HospitalFudan UniversityShanghai 200032China Biomedical Nanocenter School of Life SciencesInner Mongolia Agricultural UniversityHohhot 010011China Key Laboratory of Bio-inspired Materials and Interfacial Science Technical Institute of Physics and ChemistryChinese Academy of SciencesBeijing 100190China School of Future Technology University of Chinese Academy of SciencesBeijing 100049China Key Laboratory of Bio-inspired Smart Interfacial Science and Technology of Ministry of Education School of ChemistryBeihang UniversityBeijing 100191China

Biologically,there exist two kinds of syntheses:photosynthesis and ATP-driven biosynthesis.The light harvesting of photosynthesis is known to achieve an efficiency of〜95%by the quantum energy transfer of photons.However,how the ATP-driven biosynthesis reaches its high efficiency still remains unknown.Deoxynucleotide triphosphates(dNTPs)in polymerase chain reaction(PCR)adopt the identical way of ATP to release their energy,and thus can be employed to explore the ATP energy process.Here,using a gold nanoparticle(AuNP)enhanced PCR(AuNP-PCR),we demonstrate that the energy released by phosphoanhydride-bond(PB)hydrolysis of dNTPs is in form of photons(PB-photons)to drive DNA replication,by modulating their resonance with the average inter-AuNP distance(D).The experimental results show that both the efficiency and yield of PCR periodically oscillate with D increasing,indicating a quantized process,but not simply a thermal one.The PB-photon wavelength is further determined to 8.4 pm.All these results support that the release,transfer and utilization of bioenergy are in the form of photons.Our findings of ATP-energy quantum conversion will open a new avenue to the studies of high-efficiency bioenergy utilization,biochemistry,biological quantum physics,and even brain sciences.

关键词： intrinsic biophoton DNA replication polymerase chain reaction(PCR) gold nanoparticle high-efficiency energy utilization biological quantum physics

Change Color Effect and Spectral Properties of gold nanoparticle-cationic Surfactants System

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Chemical Research in Chinese Universities 2004年第5期20卷 523-528页

作者： JIANGZhi-liang PANHong-cheng YUANWei-en DepartmentofResourceandEnvironmentalScience GuangxiNormalUniversityGuilin541004P.R.China

The change color effect of gold nanoparticle solutions was studied by means of resonance scattering and absorption spectrometry and scan electron microscopy. The red Au nanoparticles with a size of 10 nm exhibit a resonance absorption peak and a resonance scattering peak all at 525 nm. After some inorganic electrolyte was added to a red Au nanoparticles solution, the color of the solution became blue and the absorbance at (600_700) nm was significantly increased. The ratio of the concentration of monovalent cations, at which the resonance scattering of the system at 525 nm is maximal to that of divalent cations, is in the range of 100∶1_100∶1.8. It is in good agreement with the Schulze-Hardy rule of the coagulation value of electrolyte. After adding some cationic surfactants to the above solution, the color of the solution is in deep blue, with two resonance absorption peaks at 550 and 680 nm, and a greatly enhanced resonance scattering peak at 525 nm. The experiments demonstrate that the stronger the hydrophobicity of the cationic surfactant is, the stronger the change color effect of the Au nanoparticle solution promoted by cationic surfactant is. The change color effect of Au nanoparticle solution is resulted from the increased diameter of Au nanoparticles, and the changes of resonance absorption peak and resonance scattering.

关键词： Change color effect gold nanoparticle Cationic Surfactant

Graphene-gold nanoparticle-modified Electrochemical Sensor for Detection of Kanamycin Based on Target-induced Aptamer Displacement

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Chemical Research in Chinese Universities 2018年第6期34卷 952-958页

作者： ZHENG Jingyi FENG Rongrong HE Caimei LI Xiaoxia School of Chemistry and Chemical Engineering Yah'an University Yah'an 716000 P. R. China

A highly sensitive and selective label-free electrochemical sensor was developed for the determination of kanamycin. To improve the sensitivity of the electrochemical sensor, graphene-gold nanoparticles were prepared by a one-step electrochemical coreduction process and were modified on the surface of a glassy carbon electrode. The double-stranded DNA（ds-DNA） duplex probe was immobilized onto the graphene-gold nanoparticle-modified elec-trode. The introduction of target kanamycin induced the displacement of aptamer from the ds-DNA duplex into the solution. Methylene blue（MB） as a redox indicator monitored the current change using differential pulse voltammetry Under optimal conditions, the designed electrochemical aptasensor exhibited a wide linear range from 0. i pmol/L to 10 pmol/L with a detection limit of 0.03 pmol/L for kanamycin. The experimental strategy enabled the direct analysis of milk samples, and the results showed high sensitivity and good selectivity.

关键词： kanamycin Electrochemical aptasensor Graphene gold nanoparticle Methylene blue

Detection of Lectins by Saccharide-gold nanoparticle Conjugates

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Chemical Research in Chinese Universities 2012年第6期28卷 947-952页

作者： HOU Qiong WANG Jin-e LIU Xia LI Xiao-kun MA Li-na DUAN Wu-biao WANG Zhen-xin School of Chemistry Heilongjiang University Harbin 150080 P. R. China State Key Laboratory of Electroanalytical Chemistry Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun 130022 P. R. China School of Sciences Beijing Jiaotong University Beijing 100044 P. R. China

A general functionalization strategy was reported,which enables one to conjugate saccharide（SA） on gold nanoparticle（GNP） surface without affecting SA properties.First,disulfide phenylboronic acid（Bor） functionalized GNPs（Bor@GNPs） were synthesized by the reaction of citrate stabilized GNPs of 13 nm in diameter with the mixture of Bor and pentapeptide（Cys-Ala-Leu-Asn-Asn,CALNN）.Subsequently,the SA-GNP conjugates（SA@GNPs） were prepared by coupling SA to the GNP surface via the reaction of phenylboronic acid（PBA） with the cis-diol configuration in SA.The interactions of three SA@GNPs with three lectins have been analyzed by UV-visible spectroscopic and transmission electronic microscopic（TEM） techniques,respectively.The experimental results demonstrate that SA@GNPs can efficiently bind to lectins and show a great promise as optical probes for monitoring specific affinities of lectins for SA,and detecting lectins with high sensitivity.

关键词： Saccharide gold nanoparticle Lectin Phenylboronic acid

Characterization of gold nanoparticle bioconjugation by resonance light scattering correlation spectroscopy

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Chinese Chemical Letters 2010年第10期21卷 1227-1230页

作者： Xin Qiu Li Chuan Tang Chao Qing Dong Ji Cun Ren College of Chemistry & Chemical Engineering State Key Laboratory of Metal Matrix Composites Shanghai Jiaotong University Shanghai 200240 China

gold nanoparticles （GNPs） have been widely used as probes and nanomaterials in certain biological and biomedical fields thanks to its special physical and chemical properties. However, it is still difficult to characterize GNPs-bioconjugates in solution, which has greatly limited further bioapplications of GNPs. In this study, we reported a single particle method for characterizing GNPs- biomolecules in solution using resonance light scattering correlation spectroscopy （RLSCS）. The interaction of GNPs with bovine serum albumin （BSA） and thiol-modified oligonucletides were investigated.

关键词： Resonance light scattering correlation spectroscopy gold nanoparticle Interaction Biomolecules

Graphene oxide/multi-walled carbon nanotubes/gold nanoparticle hybridfunctionalized disposable screen-printed carbon electrode to determine Cd(II)and Pb(II)in soil

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International Journal of Agricultural and Biological Engineering 2019年第3期12卷 194-200页

作者： Hui Wang Yuan Yin Guo Zhao Fernando Bienvenido Isabel M.Flores-Parrad Zhiqiang Wang Gang Liu Key Laboratory of Modern Precision Agriculture System Integration Research Ministry of Education and Key Laboratory of Agricultural Information Acquisition TechnologyMinistry of AgricultureChina Agricultural UniversityBeijing 100083China Department of Informatics University of AlmeriaAlmeriaSpain College of Computer Science and Technology Shandong University of TechnologyZibo 255049China Research Institute of Wood Industry Chinese Academy of ForestryBeijing 100091China

Cadmium(Cd)and lead(Pb)in soil or water environment cause the ecological destruction and environmental deterioration when their contents exceed the natural background values.To trace the concentrations of Cd(II)and Pb(II),a sensitive and selective electrode was developed using disposable screen-printed carbon electrode(SPE)immobilized with a composite film of reduced graphene oxide/carboxylation multi-walled carbon nanotubes/gold nanoparticle hybrid(RGO-MWNT-AuNP)throughπ-πbind.This highly conductive nano-composite layer,“RGO-MWNT-AuNP,”was characterized by scanning electron microscopy,UV-visible spectrometer,cyclic voltammetry,and electrochemical impedance spectroscopy.Square wave stripping voltammetry was applied to RGO-MWNT-AuNP/SPE to electroplate bismuth film and monitor the Cd(II)and Pb(II)simultaneously.To obtain high current responses,the detecting parameters were optimized.Under optimized conditions,the current responses showed a linear relationship with the concentrations of Cd(II)and Pb(II)in the range from 1.0 to 80.0μg/L with a lower detection limit of 0.7μg/L and 0.3μg/L(S/N=3),respectively.Finally,the prepared electrode was further employed to detect Cd(II)and Pb(II)in soil samples with good results.

关键词： electrochemical electrode heavy metal contamination screen-printed carbon electrode graphene gold nanoparticle lead cadmium multi-walled carbon nanotube

Surface-enhanced Raman Scattering from Molecules Adsorbed on Mixed Silver/gold nanoparticle Surfaces

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Chemical Research in Chinese Universities 2004年第6期20卷 817-820页

作者： FANGJing-huai HUANGYun-xia LIXia DOUXiao-ming InstituteofOpticsandPhotonics ShanghaiJiaotongUniversityShanghai200030P.R.China DepartmentofPhysics EastChinaNormalUniversityShanghai200062P.R.China DepartmentofChemistry ShanghaiTeachersUniversityShanghai200234P.R.China

Since the first discovery of Surface-Enhanced Raman Scattering(SERS) from pyridine molecules adsorbed at roughened silver electrodes in 1974 by Fleischmann et al.[1],the research of SERS has made tremendous progress i... 详细信息

关键词： Silver nanoparticle gold nanoparticle UV-Vis spectrum SERS

Sensitive detection of microRNAs using polyadenine-mediated fluorescent spherical nucleic acids and a microfluidic electrokinetic signal amplification chip

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Journal of Pharmaceutical Analysis 2022年第5期12卷 808-813页

作者： Jun Xu Qing Tang Runhui Zhang Haoyi Chen Bee Luan Khoo Xinguo Zhang Yue Chen Hong Yan Jincheng Li Huaze Shao Lihong Liu NMPA Key Laboratory for Research and Evaluation of Drug Metabolism Guangdong Provincial Key Laboratory of New Drug ScreeningSchool of Pharmaceutical SciencesSouthern Medical UniversityGuangzhou510515China The Second Clinical Medical School Southern Medical UniversityGuangzhou510515China Department of Biomedical Engineering City University of Hong KongHong Kong999077China

The identification of tumor-related microRNAs(miRNAs)exhibits excellent promise for the early diagnosis of cancer and other bioanalytical applications.Therefore,we developed a sensitive and efficient biosensor using polyadenine(polyA)-mediated fluorescent spherical nucleic acid(FSNA)for miRNA analysis based on strand displacement reactions on gold nanoparticle(AuNP)surfaces and electrokinetic signal amplification(ESA)on a microfluidic chip.In this FSNA,polyA-DNA biosensor was anchored on AuNP surfaces via intrinsic affinity between adenine and Au.The upright conformational polyA-DNA recognition block hybridized with 6-carboxyfluorescein-labeled reporter-DNA,resulting in fluorescence quenching of FSNA probes induced by AuNP-based resonance energy transfer.Reporter DNA was replaced in the presence of target miRNA,leading to the recovery of reporter-DNA fluorescence.Subsequently,reporter-DNAs were accumulated and detected in the front of with Nafion membrane in the microchannel by ESA.Our method showed high selectivity and sensitivity with a limit of detection of 1.3 pM.This method could also be used to detect miRNA-21 in human serum and urine samples,with recoveries of 104.0%-113.3% and 104.9%-108.0%,respectively.Furthermore,we constructed a chip with three parallel channels for the simultaneous detection of multiple tumor-related miRNAs(miRNA-21,miRNA-141,and miRNA-375),which increased the detection efficiency.Our universal method can be applied to other DNA/RNA analyses by altering recognition sequences.

关键词： MicroRNAs Microfluidic chip Electrokinetic signal amplification Polyadenine-DNA gold nanoparticle