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Nano Research 2017年第1期10卷 108-120页

作者： Juan Peng Liqiang Liu Liguang Xu Shanshan Song Hua Kuang Gang Cui Chuanlai Xu State Key Laboratory of Food Science and Technology Jiangnan University Wuxi 214122 China School of Food Science and Technology Jiangnan University Wuxi 214122 China

For rapid and simultaneous detection of （fluoro）quinolones, a broadly specific monoclonal antibody （mAb） that recognizes 32 （fluoro）quinolone antibiotics was prepared using a mixture of a norfloxacin derivative and a sarfloxacin derivative as the hapten. An immunochromatographic strip based on gold nanoparticles （AuNPs） was then assembled with goat anti-mouse antibody and antigen （sarfloxacin coupled to ovalbumin）, used to form the C line and T line, respectively. This antigen competes with the （fluoro）quinolones in a sample incubated with mAbs labeled with AuNPs. The strip can detect 32 （fluoro）quinolones including oxolinic acid, nalidixic acid, miloxacin, pipemidic acid, piromidic acid, rosoxacin, cinoxacin, norfloxacin, pefloxacin, lomfloxacin, enofloxacin, fleroxacin, ciprofloxacin, enrofloxacin, dafloxacin, orbifloxacin, sparfloxacin, gemifloxacin, besifloxacin, balofloxacin, gatifloxacin, moxifloxacin, nadifloxacin, ofloxacin, marbofloxacin, flumequine, pazufloxacin, prulifloxacin, sarafloxacin, difloxacin, trovafloxacin, and tosufloxacin in milk within 10 min with the naked eye. The cut-off values of the strip range from 1 to 100 ng/mL and the limits of detection are 0.1- 10 ng/mL. The strip does not cross-react with antibiotics including tetracycline, sulfamethazine, ampicillin, erythromycin, aflatoxin B1, or gentamicin. In short, this immunochromatographic strip is a very useful tool for the primary screening of （fluoro）quinolones in milk.

关键词： (fluoro)quinolones gold nanoparticle monoclonal antibody paper sensor

gold nanoparticle-based Colorimetric Assay for Determination of Lead(Ⅱ) in Aqueous Media

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Chemical Research in Chinese Universities 2010年第2期26卷 194-197页

作者： LI Xiao-kun WANG Zhen-xin State Key Laboratory of Electroanalytical Chemistry Changehun Institute of Applied Chemistry Chinese Academy of Sciences Changchun 130022 R R. China Graduate School of the Chinese Academy ofScienees Beoing 100049 P. R. China

The authors presented a simple colorimetric assay for the detection of toxic heavy metal lead（Ⅱ） ion（Pb2＋） Pentapeptide, cysteine-alanine-leacine-asparagine-asparagine（CALNN）, functionalized gold nanoparticles（GNPs） were aggregated in the presence of the divalent metal ion in solution by an ion-templated chelation process, which caused an easily measurable change in the absorption spectrum of the particles. Typically, mono-dispersing GNPs exhibit an absorption band at 522 nm, corresponding to a red color solution, while aggregated GNPs have it at longer wavelengths, corresponding to a purple or blue color solution. The chelation/aggregation process is reversible via the addition of a strong metal ion chelator such as EDTA. Highly selective and sensitive detection of Pb^2＋ in aqueous solution is thus provided. A detection limit of 0.1 μmol/L of Pb^2＋ was demonstrated.

关键词： gold nanoparticle Peptide Colorimetric assay Lead(Ⅱ)

Recent progress in gold nanoparticle-based biosensing and cellular imaging

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Science China Chemistry 2016年第7期59卷 783-793页

作者： Haiyang Peng Hao Tang Jianhui Jiang State Key Laboratory of Chemo/Bio-Sensing and Chemometrics College of Chemistry and Chemical Engineering Hunan University

gold nanoparticles(Au NPs) have been extensively used in optical biosensing and bioimaging due to the unique optical properties. Biological applications including biosensing and cellular imaging based on optical properties of Au NPs will be reviewed in the paper. The content will focus on detection principles, advantages and challenges of these approaches as well as recent advances in this field.

关键词： gold nanoparticle optical functionalization biosensing cellular imaging

Demonstration of biophoton-driven DNA replication via gold nanoparticle-distance modulated yield oscillation

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Nano Research 2021年第1期14卷 40-45页

作者： Na Li Daoling Peng Xianjing Zhang Yousheng Shu Feng Zhang Lei Jiang Bo Song School of Optical-Electrical Computer Engineering University of Shanghai for Science and TechnologyShanghai 200093China State Key Laboratory of Respiratory Disease Key Laboratory of Oral MedicineGuangzhou Institute of Oral DiseaseStomatology HospitalDepartment of Biomedical EngineeringSchool of Basic Medical SciencesGuangzhou Medical UniversityGuangzhou 511436China School of Chemistry South China Normal UniversityGuangzhou 510006China State Key Laboratory of Medical Neurobiology Institute for Translational Brain ResearchMOE Frontiers Center for Brain ScienceDepartment of NeurologyHuashan HospitalFudan UniversityShanghai 200032China Biomedical Nanocenter School of Life SciencesInner Mongolia Agricultural UniversityHohhot 010011China Key Laboratory of Bio-inspired Materials and Interfacial Science Technical Institute of Physics and ChemistryChinese Academy of SciencesBeijing 100190China School of Future Technology University of Chinese Academy of SciencesBeijing 100049China Key Laboratory of Bio-inspired Smart Interfacial Science and Technology of Ministry of Education School of ChemistryBeihang UniversityBeijing 100191China

Biologically,there exist two kinds of syntheses:photosynthesis and ATP-driven *** light harvesting of photosynthesis is known to achieve an efficiency of〜95%by the quantum energy transfer of ***,how the ATP-driven biosynthesis reaches its high efficiency still remains *** triphosphates(dNTPs)in polymerase chain reaction(PCR)adopt the identical way of ATP to release their energy,and thus can be employed to explore the ATP energy ***,using a gold nanoparticle(AuNP)enhanced PCR(AuNP-PCR),we demonstrate that the energy released by phosphoanhydride-bond(PB)hydrolysis of dNTPs is in form of photons(PB-photons)to drive DNA replication,by modulating their resonance with the average inter-AuNP distance(D).The experimental results show that both the efficiency and yield of PCR periodically oscillate with D increasing,indicating a quantized process,but not simply a thermal *** PB-photon wavelength is further determined to 8.4 *** these results support that the release,transfer and utilization of bioenergy are in the form of *** findings of ATP-energy quantum conversion will open a new avenue to the studies of high-efficiency bioenergy utilization,biochemistry,biological quantum physics,and even brain sciences.

关键词： intrinsic biophoton DNA replication polymerase chain reaction(PCR) gold nanoparticle high-efficiency energy utilization biological quantum physics

Change Color Effect and Spectral Properties of gold nanoparticle-cationic Surfactants System

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Chemical Research in Chinese Universities 2004年第5期20卷 523-528页

作者： JIANGZhi-liang PANHong-cheng YUANWei-en DepartmentofResourceandEnvironmentalScience GuangxiNormalUniversityGuilin541004P.R.China

The change color effect of gold nanoparticle solutions was studied by means of resonance scattering and absorption spectrometry and scan electron microscopy. The red Au nanoparticles with a size of 10 nm exhibit a resonance absorption peak and a resonance scattering peak all at 525 nm. After some inorganic electrolyte was added to a red Au nanoparticles solution, the color of the solution became blue and the absorbance at (600_700) nm was significantly increased. The ratio of the concentration of monovalent cations, at which the resonance scattering of the system at 525 nm is maximal to that of divalent cations, is in the range of 100∶1_100∶1.8. It is in good agreement with the Schulze-Hardy rule of the coagulation value of electrolyte. After adding some cationic surfactants to the above solution, the color of the solution is in deep blue, with two resonance absorption peaks at 550 and 680 nm, and a greatly enhanced resonance scattering peak at 525 nm. The experiments demonstrate that the stronger the hydrophobicity of the cationic surfactant is, the stronger the change color effect of the Au nanoparticle solution promoted by cationic surfactant is. The change color effect of Au nanoparticle solution is resulted from the increased diameter of Au nanoparticles, and the changes of resonance absorption peak and resonance scattering.

关键词： Change color effect gold nanoparticle Cationic Surfactant

gold nanoparticle stabilization within tailored cubic mesoporous silica:Optimizing alcohol oxidation activity

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Chinese Journal of Catalysis 2017年第3期38卷 545-553页

作者：洪伟闫晓庆李仁宏范杰浙江大学化学系浙江省应用化学重点实验室浙江杭州310027 浙江理工大学先进纺织材料与制备技术教育部重点实验室浙江杭州310018

Stabilizing gold nanoparticles（AuNPs） within a desired size range is critical to realize their promising catalytic performance in many important ***,we investigate the anti-sintering properties of cubic mesoporous silica（FDU-12） as a function of pore entrance *** adjustments to the type of organic template and reaction temperature enable the successful synthesis of FDU-12 with controllable entrance sizes（ 3,3-5 and 7 nm）.Excellent anti-sintering properties are observed for FDU-12 with a sub-5-nm entrance size（3-5 nm） over a wide loading concentration（1.0-8.3 wt%） and the AuNPs can be stabilized within a 4.5-5.0-nm range after calcination at 550 ℃in air for 5 *** entrance size（ 3 nm） prevents ingress of 3-nm AuNPs to the mesopores and results in low loading capacity and ***,FDU-12 possessing a larger entrance size（7 nm） shows promising anti-sintering properties at high loading concentrations,although catalytic performance is significantly lost at lower concentrations（e.g.2.1 wt%,14.2 ± 5.5 nm）.Different anti-sintering mechanisms are proposed for each of the different FDU-12 entrance ***,catalytic data indicates that the obtained 4.5-nm AuNPs supported on FDU-12 with a sub-5-nm entrance size exhibit excellent mass-specific activity（1544 mmol g_（Au）^（-1） h^（-1）） and selectivity（ 99%）at 230 ℃ for the gas-phase selective oxidation of cyclohexanol.

关键词： gold nanoparticle Cubic mesoporous silica Entrance size Anti-sintering property Alcohol oxidation

Graphene-gold nanoparticle-modified Electrochemical Sensor for Detection of Kanamycin Based on Target-induced Aptamer Displacement

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Chemical Research in Chinese Universities 2018年第6期34卷 952-958页

作者： ZHENG Jingyi FENG Rongrong HE Caimei LI Xiaoxia School of Chemistry and Chemical Engineering Yah'an University Yah'an 716000 P. R. China

A highly sensitive and selective label-free electrochemical sensor was developed for the determination of kanamycin. To improve the sensitivity of the electrochemical sensor, graphene-gold nanoparticles were prepared by a one-step electrochemical coreduction process and were modified on the surface of a glassy carbon electrode. The double-stranded DNA（ds-DNA） duplex probe was immobilized onto the graphene-gold nanoparticle-modified elec-trode. The introduction of target kanamycin induced the displacement of aptamer from the ds-DNA duplex into the solution. Methylene blue（MB） as a redox indicator monitored the current change using differential pulse voltammetry Under optimal conditions, the designed electrochemical aptasensor exhibited a wide linear range from 0. i pmol/L to 10 pmol/L with a detection limit of 0.03 pmol/L for kanamycin. The experimental strategy enabled the direct analysis of milk samples, and the results showed high sensitivity and good selectivity.

关键词： kanamycin Electrochemical aptasensor Graphene gold nanoparticle Methylene blue

Detection of Lectins by Saccharide-gold nanoparticle Conjugates

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Chemical Research in Chinese Universities 2012年第6期28卷 947-952页

作者： HOU Qiong WANG Jin-e LIU Xia LI Xiao-kun MA Li-na DUAN Wu-biao WANG Zhen-xin School of Chemistry Heilongjiang University Harbin 150080 P. R. China State Key Laboratory of Electroanalytical Chemistry Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun 130022 P. R. China School of Sciences Beijing Jiaotong University Beijing 100044 P. R. China

A general functionalization strategy was reported,which enables one to conjugate saccharide（SA） on gold nanoparticle（GNP） surface without affecting SA ***,disulfide phenylboronic acid（Bor） functionalized GNPs（Bor@GNPs） were synthesized by the reaction of citrate stabilized GNPs of 13 nm in diameter with the mixture of Bor and pentapeptide（Cys-Ala-Leu-Asn-Asn,CALNN）.Subsequently,the SA-GNP conjugates（SA@GNPs） were prepared by coupling SA to the GNP surface via the reaction of phenylboronic acid（PBA） with the cis-diol configuration in *** interactions of three SA@GNPs with three lectins have been analyzed by UV-visible spectroscopic and transmission electronic microscopic（TEM） techniques,*** experimental results demonstrate that SA@GNPs can efficiently bind to lectins and show a great promise as optical probes for monitoring specific affinities of lectins for SA,and detecting lectins with high sensitivity.

关键词： Saccharide gold nanoparticle Lectin Phenylboronic acid

Convective Assembly of Linear gold nanoparticle Arrays at the Micron Scale for Surface Enhanced Raman Scattering

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Nano Research 2011年第11期4卷 1117-1128页

作者： Lihua Qian Ronobir Mookherjee Nevada Nanotechnology Center Howard R. Hughes College of Engineering University of Nevada Las Vegas Nevada 89154-4026 USA Advanced Technologies Academy 2501 Vegas Drive Las Vegas NV 89106 USA

A convective assembly technique at the micron scale analogous to the writing action of a ＂pipette pen＂ has been developed for the linear assembly of gold nanoparticle strips with micron scale width and millimeter scale length for surface enhanced Raman scattering （SERS）. The arrays with interparticle gaps smaller than 3 nm are hexagonally stacked in the vicinity of the pipette tip. Variable numbers of stacked layers and clean surfaces of the assembled nanoparticles are obtained by optimizing the velocity of the pipette tip. The SERS properties of tile assembled nanoparticle arrays rely on their stacking number and surface cleanliness.

关键词： Convective assembly gold nanoparticle surface enhanced Raman scattering pipette writing

Characterization of gold nanoparticle bioconjugation by resonance light scattering correlation spectroscopy

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Chinese Chemical Letters 2010年第10期21卷 1227-1230页

作者： Xin Qiu Li Chuan Tang Chao Qing Dong Ji Cun Ren College of Chemistry & Chemical Engineering State Key Laboratory of Metal Matrix Composites Shanghai Jiaotong University Shanghai 200240 China

gold nanoparticles （GNPs） have been widely used as probes and nanomaterials in certain biological and biomedical fields thanks to its special physical and chemical properties. However, it is still difficult to characterize GNPs-bioconjugates in solution, which has greatly limited further bioapplications of GNPs. In this study, we reported a single particle method for characterizing GNPs- biomolecules in solution using resonance light scattering correlation spectroscopy （RLSCS）. The interaction of GNPs with bovine serum albumin （BSA） and thiol-modified oligonucletides were investigated.

关键词： Resonance light scattering correlation spectroscopy gold nanoparticle Interaction Biomolecules