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gmgpdh12,a mitochondrial FAD-GPDH from soybean, increases salt and osmotic stress resistance by modulating redox state and respiration

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The Crop Journal 2021年第1期9卷 79-94页

作者： Ying Zhao Xin Li Zexin Zhang Wenjing Pan Sinan Li Yun Xing Wanying Xin Zhanguo Zhang Zhenbang Hu Chunyan Liu Xiaoxia Wu Zhaoming Qi Dawei Xin Qingshan Chen College of Agriculture Northeast Agricultural UniversityHarbin 150030HeilongjiangChina Key Lab of Maize Genetics and Breeding Heilongjiang Academy of Agricultural SciencesHarbin 150030HeilongjiangChina College of Agriculture Heilongjiang Bayi Agricultural UniversityDaqing 163319HeilongjiangChina

In plants,glycerol-3-phosphate dehydrogenase(GPDH)catalyzes the interconversion of glycerol-3-phosphate(G3P)and dihydroxyacetone phosphate(DHAP)coupled to the reduction/oxidation of the nicotinamide adenine dinucleotide(NADH)pool,and plays a central role in glycerolipid metabolism and stress *** studies have focused mainly on the NAD+-dependent GPDH isoforms,neglecting the role of flavin adenine dinucleotide(FAD)-dependent *** isolated and characterized three mitochondrialtargeted FAD-GPDHs in soybean,of which one isoform(gmgpdh12)showed a significant transcriptional response to NaCl and mannitol treatments,suggesting the existence of a major FAD-GPDH isoform acting in soybean responses to salt and osmotic *** enzyme kinetic assay showed that the purified gmgpdh12 protein possessed the capacity to oxidize G3P to DHAP in the presence of *** and RNA interference of gmgpdh12 in soybean hairy roots resulted in elevated tolerance and sensitivity to salt and osmotic stress,respectively.G3P contents were significantly lower in gmgpdh12-overexpressing hair roots and higher in knockdown hair roots,indicating that gmgpdh12 was essential for G3P catabolism.A significant perturbation in redox status of NADH,ascorbic acid(ASA)and glutathione(GSH)pools was observed in gmgpdh12-knockdown plants under stress *** impaired redox balance was manifested by higher reactive oxygen species generation and consequent cell damage or death;however,overexpressing plants showed the opposite results for these ***12 overexpression contributed to maintaining constant respiration rates under salt or osmotic stress by regulating mRNA levels of key mitochondrial respiratory *** study provides new evidence for the roles of mitochondria-localized gmgpdh12 in conferring resistance to salt or osmotic stress by maintaining cellular redox homeostasis,protecting cells and respiration from oxidative injury.

关键词： Redox homeostasis Respiration characteristics gmgpdh12 Salt stress Osmotic stress

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