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Deficiency of Antisense lncRNA gm48853 Resulted in Embryonic Lethality and Impaired Placental Development in Mice

Reproductive and Developmental Medicine 2019年第1期3卷 5-10页

作者： Qi-Yun Qin Wei-Jia Zeng Chu-Yue Peng Yu-Fang Zheng Hong-Yan Wang The Institute of Reproduction and Developmental Biology Obstetrics and Gynecology HospitalFudan UniversityShanghai 200438China Institute of Developmental Biology and Molecular Medicine Fudan UniversityShanghai 200438China The State Key Laboratory of Genetic Engineering MOE Key Laboratory of Contemporary AnthropologySchool of Life ScienceFudan UniversityShanghai 200438China Children’s Hospital and Institutes of Biomedical Sciences Fudan UniversityShanghai 200438China

Objective:Long noncoding RNAs(lncRNAs)play important roles in embryonic development and in various ***48853 is a predicted natural antisense lncRNA gene of unknown *** present study aimed to investigate its expression and ***:Quantitative reverse transcription-polymerase chain reaction and sequencing were used to detect the expression of gm48853 antisense *** and eosin staining and pathological analysis were applied to evaluate the effects of gm48853 mutations on embryonic/placental development.5-Ethynyl-2’-deoxyuridine(EdU)and terminal deoxynucleotidyl transferase deoxy-UTP-nick-end labeling assays were used to analyze the status of cell proliferation and apoptosis in gm48853-deficient embryos and ***:PiggyBac(PB)transposon insertion into the intron of gm48853 resulted in a significant decrease in gm48853 expression and embryonic lethality at *** gm48853 mutations led to severe growth retardation of embryos and impaired the morphogenesis of the placental *** addition,cell proliferation was dramatically decreased in gm48853^(PB/PB) embryos and placentas,and apoptosis was radically increased in the mutant embryos but not in the mutant ***:Antisense lncRNA gm48853 may regulate embryonic/placental development by enhancing cell proliferation and/or inhibiting apoptosis.

关键词： Antisense lncRNA Embryonic Development gm48853 Placental Development

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Deficiency of Antisense lncRNAResulted in Embryonic Lethality and Impaired Placental Development in Mice

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生殖与发育医学（英文） 2019年第1期 5-10页

Objective: Long noncoding RNAs (lncRNAs) play important roles in embryonic development and in various ***48853 is a predicted natural antisense lncRNA gene of unknown function. The present study aimed to investigate its expression and ***: Quantitative reverse transcription-polymerase chain reaction and sequencing were used to detect the expression ofgm48853 antisense lncRNA. Hematoxylin and eosin staining and pathological analysis were applied to evaluate the effects ofgm48853 mutations on embryonic/placental development. 5-Ethynyl-2’-deoxyuridine (EdU) and terminal deoxynucleotidyl transferase deoxy-UTP-nick-end labeling assays were used to analyze the status of cell proliferation and apoptosis ingm48853-deficient embryos and ***: PiggyBac (PB) transposon insertion into the intron ofgm48853 resulted in a significant decrease ingm48853 expression and embryonic lethality at midgestation. Homozygousgm48853 mutations led to severe growth retardation of embryos and impaired the morphogenesis of the placental labyrinth. In addition, cell proliferation was dramatically decreased ingm48853PB/PB embryos and placentas, and apoptosis was radically increased in the mutant embryos but not in the mutant ***: Antisense lncRNAgm48853 may regulate embryonic/placental development by enhancing cell proliferation and/or inhibiting apoptosis.

关键词： Antisense lncRNA Embryonic Development gm48853 Placental Development

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