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Truncated glycoprotein e of varicella-zoster virus is an ideal immunogen for escherichia coli-based vaccine design

Science China(Life Sciences) 2023年第4期66卷 743-753页

作者： Tingting Chen Jie Sun Sibo Zhang Tingting Li Liqin Liu Wenhui Xue Lizhi Zhou Siting Liang Zhili Yu Qingbing Zheng Hai Yu Tong Cheng Jun Zhang Ying Gu Shaowei Li Ningshao Xia State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics National Institute of Diagnostics and Vaccine Development in Infectious DiseasesSchool of Life Sciences&School of Public HealthXiamen UniversityXiamen 361102China Xiang An Biomedicine Laboratory Xiamen 361102China Research Unit of Frontier Technology of Structural Vaccinology Chinese Academy of Medical SciencesXiamen 361102China

Varicella-zoster virus(VZV)is a highly infectious agent responsible for both varicella and herpes zoster disease.Despite high efficacy,there remain safety and accessibility concerns with the licensed vaccines.Here,we sought to produce a VZV g e immunogen using an e.coli expression system.We found that the soluble expression and yield of g e protein could be enhanced via C-terminal truncations to the protein,thereby facilitating a robust and scalable purification process for the purpose of vaccine manufacturing.The lead truncated g e(aa 31–358),hereafter referred to as tg e,was a homogenous monomer in solution and showed excellent antigenicity.Finally,we assessed and compared the immunogenicity of tg e with commercial v Oka LAV and Shingrix vaccine.We found that aluminum-adjuvanted tg e was immunogenic as compared with v Oka LAV.When adjuvanted with AS01B,a two-dose immunization of tg e showed comparable or better potency in antibody responses and cell-mediated immunity with those of the Shingrix vaccine at the same dosage,especially in terms of the proportion of IFN-γ-expressing CD4^(+)T cells.In conclusion,this method of e.coli-mediate tg e expression offers a cost-effective and scalable strategy to generate an ideal VZV g e immunogen for the development of both varicella and zoster vaccines.

关键词： varicella-zoster virus glycoprotein e escherichia coli vaccine

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expression of Pseudorabies Virus ge Core epitopes in escherichia coli Strain BL21 and Utilization of Indirect PRV ge-eLISA

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Agricultural Biotechnology 2014年第4期3卷 39-44页

作者： Guangjun GUO Sufang LU Guanggang QU Feng LI Lin DONG Yanli BI Jinliang WANG Feng WeI Na TANG Chunling ZHANG Zhuang DING Zhiqiang SHeN Shandong Binzhou Animal Science & Veterinary Medicine Academy College of Veterinary Medicine Jinlin University Shandong Lvdu Bio-Science & Technology Co. Ltd.

Pseudorabies virus glycoprotein e （PRV ge） has been recognized as a suitable diagnostic antigen for pseudorabies. In order to produce ge antigen in large quantities and at low cost, a gene fragment encoding PRV ge core epitopes was expressed in e. coli BL21 expression system. SDS-PAGe and Western Blotting revealed that the expression product in culture supematant of e. coli BL21 was a recombinant protein, approximately 51.8 Kd. At 5 h post-induction, protein concentration assay showed that the expression product amounted to 1.65 mg/ml, accounting for 24. 17% of total proteins in the culture supematant. An indirect PRV ge-eLISA was established by using the recombinant expression product as a coating antigen. Cross-reactivity assay showed that this antigen was PRV specific. In addition, the assay was consistently reproducible. Comparison of detection results of 240 serum samples between PRV ge-eLISA and a commercially available PRV diagnostic kit showed that there was no significant difference between these two methods （P 〉 0.05 ）.

关键词： Pseudorabies virus glycoprotein e PRV strain SA Gene expression eLISA

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Comprehensive diagnosis of variant pseudorabies virus epidemic in central Shandong province, China

Comprehensive diagnosis of variant pseudorabies virus epidem...

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中国畜牧兽医学会兽医病理学分会第二十一次学术研讨会暨中...

作者： Dongfang Hu Zhendong Zhang Lin Lv Sidang Liu College of Animal Science and Technology of Shandong Agricultural University

＜正＞Objective An epidemic that mainly endangered 3–7-day-old piglets struck many farms in Shandong province in2013 and caused heavy losses.To identify the pathogenesis,the type of lesions,and the causative agent,systemic examinations were performed.Materials and methods The study was conducted on a representative farm,and 10 of the dead piglets were necropsied in a

关键词： Bartha-K61 glycoprotein e variant pathology pseudorabies virus serology

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