检索结果-南通市图书馆

The AMPK and AKT/GSK3β pathways are involved in recombinant proteins fibroblast growth factor 1(rFGF1 and rFGF1a) improving glycolipid metabolism in rainbow trout(Oncorhynchus mykiss) fed a high carbohydrate diet

引用

Animal Nutrition 2024年第2期17卷 11-24页

作者： Huixia Yu Shuo Geng Shuai Li Yingwei Wang Xin Ren Debin Zhong Haolin Mo Mingxing Yao Jiajia Yu Yang Li Lixin Wang College of Animal Science and Technology Northwest A&F University Meixian Aquaculture Farm of Shitouhe Reservoir Administration

Fibroblast growth factor 1(FGF1) regulates vertebrate cell growth, proliferation and differentiation, and energy metabolism. In this study, we cloned rainbow trout(Oncorhynchus mykiss) fgf1 and fgf1a, prepared their recombinant proteins(rFGF1 and rFGF1a), and described the molecular mechanisms by which they improve glycolipid metabolism in carnivorous fish. A 31-d feeding trial was conducted to investigate whether they could enhance glycolipid metabolism in rainbow trout on high-carbohydrate diets(HCD). A total of 720 rainbow trout(8.9 ± 0.5 g) were equally divided into 4 groups: the chow diet(CD) group injected with PBS, the HCD group injected with PBS, the HCD group injected with rFGF1(400 ng/g body weight), and the HCD group injected with rFGF1a(400 ng/g body weight). The results showed that short-term HCD had a significant positive effect on the specific growth rate(SGR) of rainbow trout(P glycogen content under HCD, and increased glucose uptake by hepatocytes. We observed a decrease in mRNA levels for pepck, g6pase, and pygl, along with an increase in mRNA levels for gys, glut2, and gk in the liver. Furthermore, these proteins regulated hepatic gluconeogenesis and glycogen synt

关键词： Rainbow trout Recombinant proteins Fibroblast growth factor 1 glycogen synthesis Lipid deposition

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Loss of hnRNP A1 in murine skeletal muscle exacerbates high-fat diet-induced onset of insulin resistance and hepatic steatosis

引用

Journal of Molecular Cell Biology 2020年第4期12卷 277-290页

作者： Mingxia Zhao Lihong Shen Zijun Ouyang Manru Li Guoliang Deng Chenxi Yang Wei Zheng Lingdong Kong Xuefeng Wu Xudong Wu Wenjie Guo Ye Yin Qiang Xu Yang Sun State Key Laboratory of Pharmaceutical Biotechnology Department of Biotechnology and Pharmaceutical SciencesSchool of Life SciencesNanjing UniversityNanjing 210023China Key Laboratory of Human Functional Genomics of Jiangsu Province Department of Biochemistry and Molecular BiologyNanjing Medical UniversityNanjing 210029China State Key Laboratory of Drug Research Shanghai Institute of Materia MedicaChinese Academy of SciencesShanghai 201203China

Impairment of glucose(Glu)uptake and storage by skeletal muscle is a prime risk factor for the development of metabolic *** nuclear ribonucleoprotein A1(hnRNP Al)is a highly abundant RNA-binding protein that has been implicated in diverse cellular *** aim of this study was to investigate the function of hnRNP A1 on muscle tissue insulin sensitivity and systemic Glu *** results showed that conditional deletion of hnRNP Al in the muscle gave rise to a severe insulin resistance phenotype in mice fed a high-fat diet(HFD).Conditional knockout mice fed a HFD showed exacerbated obesity,insulin resistance,and hepatic *** vitro interference of hnRNP Al in C2C12 myotubes impaired insulin signal transduction and inhibited Glu uptake,whereas hnRNP Al overexpression in C2C12 myotubes protected against insulin resistance induced by supraphysiological concentrations of *** expression and stability of glycogen synthase(gysl)mRNA were also decreased in the absence of hnRNP A ***,hnRNP Al interacted with gys l and stabilized its mRNA,thereby promoting glycogen synthesis and maintaining the insulin sensitivity in muscle *** together,our findings are the first to show that reduced expression of hnRNP Al in skeletal muscle affects the metabolic properties and systemic insulin sensitivity by inhibiting glycogen synthesis.

关键词： hnRNP A1 insulin resistance hepatic steatosis glycogen synthesis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Molecular Cloning and Construction of agp Gene Deletion-mutant in Cyanobacterium Synechocystis sp. PCC 6803

引用

Acta Botanica Sinica 2001年第5期43卷 512-516页

作者：吴桂芳沈忠耀吴庆余赵南明清华大学生物科学与技术系北京100084 清华大学化工系生物化工研究所北京100084

The agp gene encoding the ADP-glucose pyrophosphorylase involved in cyanobacterial glycogen synthesis was amplified by PCR. The resulting agp fragment was cloned in plasmid pUC118 to generate plasmid pUCA. Part of the fragment within the agp DNA was deleted and replaced by an erythromycin resistance cassette to generate plasmid pUCAE, which was used to transform the Synechocystis sp. PCC 6803 wild-type strain and a mutant with resistance to erythromycin was obtained. PCR analysis of the genomic DNA from the resulting mutant indicated that the appropriate deletion and insertion indeed had occurred. The cell growth and Chl a, glycogen content in the mutant showed difference from those in the wild-type strain. The obtained biomass as well as the Chl a content in the mutant strain was higher than that of the wild-type strain, which suggested that the photosynthesis efficiency in the agp(-) strain was higher than that in the wild-type strain. No glycogen was found in the mutant, providing evidence for the correction of the mutant in physiological level.

关键词： cyanobacterium Synechocystis sp PCC 6803 agp cloning deletion mutant glycogen synthesis photosynthesis

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The protein phosphatase 1 complex is a direct target of AKT linking insulin signaling to hepatic glycogen deposition

The protein phosphatase 1 complex is a direct target of AKT ...

引用

中国生物化学与分子生物学会2019年全国学术会议暨学会成立四十周年

作者： Qiqi Li Qiuye Zhao Junyu Zhang Linkang Zhou Wenhao Zhang Boon Tin Chua Yan Chen Li Xu Peng Li State Key Laboratory of Membrane Biology and Tsinghua-Peking Center for Life Sciences School of Life Sciences Tsinghua University The Institute of Metabolism and Integrative Biology Fudan University CAS Key Laboratory of Nutrition Metabolism and Food Safety Shanghai Institute of Nutrition and Health Chinese Academy of Sciences

Insulin-stimulated hepatic glycogen synthesis is central to glucose homeostasis. Here, we identify that PPP1 R3 g, a regulatory subunit of protein phosphatase 1(PP1), is directly phosphorylated by AKT. PPP1 R3 g phosphorylation oscillates with fasting-refeeding cycle, and is required for insulin-stimulated dephosphorylation(hence activation) of glycogen synthase(GS) in hepatocytes. We show that knockdown of PPP1 R3 g significantly blunted insulin response. Introduction of wild-type PPP1 R3 g, but not phosphorylation-defective mutant, leads to increase blood glucose clearance, hepatic glycogen deposition, and improved insulin sensitivity in vivo. Mechanistically, phosphorylated PPP1 R3 g displays increased binding for, and promotes dephosphorylation of, phospho-GS. Furthermore, PPP1 R3 b, another regulatory subunit of PP1 binds to the dephosphorylated GS, thereby relaying insulin stimulation to hepatic glycogen deposition. Importantly, this PP1-mediated signaling cascade is independent of GSK3. We have thus revealed a novel regulatory axis consisting of insulin/AKT/PPPP1 R3 g/PPP1 R3 b that operates in parallel to the GSK3-dependent route, controlling glycogen synthesis and glucose homeostasis in insulin signaling.

关键词： glycogen synthesis hepatocytes insulin signal postprandial glucose regulation protein phosphorylation

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：