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Exploration of the metabolic flexibility of glycogen accumulating organisms through metatranscriptome analysis and metabolic characterization

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Journal of Environmental Sciences 2023年第4期126卷 234-248页

作者： Bin Zhao Yanping Yang Chen Zhao Chunchun Zhang Zhaohui Zhang Liang Wang Shang Wang Jingfeng Wang School of Environmental Science Engineering Tiangong UniversityTianjin 300387China Department of Hygienic Toxicology and Environmental Hygiene Tianjin Institute of Environmental and Operational MedicineTianjin 300201China

glycogen accumulating organisms(GAOs) are closely related to the deterioration of enhanced biological phosphorus removal systems. However, the metabolic mechanisms that drive GAOs remain unclear. Here, the two-thirds supernatant of a reactor were decanted following the anaerobic period to enrich GAOs. Long-term monitoring demonstrated that the system was stable and exhibited typical characteristics of GAOs metabolism. Acetate was completely consumed after 60 min of the anaerobic phase. The level of glycogen decreased from 0.20 to 0.14 g/gSS during the anaerobic phase, whereas the level of glycogen significantly increased to 0.21g/gSS at the end of the aerobic period. Moreover, there was almost no phosphate release and absorption in the complete periods, thus confirming the successful construction of a GAOs enrichment system. Microbial community analysis demonstrated that Ca. Contendobacter was among the core functional genera and showed the highest activity among all of the communities. Furthermore, our study is the first to identify the involvement of the ethyl-malonyl-CoA pathway in the synthesis of polyhydroxyvalerate via croR, ccr, ecm, mcd, mch and mcl genes. The Embden-Meyerhof-Parnas(EMP) pathway was preferentially used via glgP. Furthermore, the glyoxylate cycle was the main source of ATP under anaerobic conditions, whereas the tricarboxylic acid cycle provided ATP under aerobic conditions. aceA and mdh appeared to be major modulators of the glyoxylate pathway for controlling energy flow. Collectively, our findings not only revealed the crucial metabolic mechanisms in a GAOs enrichment system but also provided insights into the potential application of Ca. Contendobacter for wastewater treatment.

关键词： glycogen accumulating organisms Intracellular storage Metatranscriptome Bacterial community Glyoxylate pathway

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Monitoring microbial community structure and succession of an A/O SBR during start-up period using PCR-DGGE

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Journal of Environmental Sciences 2009年第2期21卷 223-228页

作者： WANG Xiuheng,ZHANG Kun,REN Nanqi,LI Nan,REN Lijiao State Key Laboratory of Urban Water Resource and Environment,Harbin Institute of Technology,Harbin 150090,China. State Key Laboratory of Urban Water Resource and Environment Harbin Institute of Technology Harbin 150090 China

Polymerase chain reaction （PCR）-denaturing gradient gel electrophoresis （DGGE） protocol was employed for revealing microbial community structure and succession in a sequential anaerobic and aerobic reactor performing enhanced biological phosphorus removal （EBPR） during start-up period. High phosphorus removal was achieved after 15 d. On day 30, phosphorus removal efficiency reached to 83.2% and the start-up was finished. DGGE profiles of periodical sludge samples showed that dominant microbial species were 19 OTUs （operational taxonomy units）. Unweighted pair-group method using arithmetic averages （UPGMA） clustering analysis revealed that rapid community succession correlated to low phosphorus removal rate and high phosphorus removal efficiency reflected on steady community structure. Sequencing results indicated that determined sequences （12 OTUs） belonged to Proteobacterium, Actinobacteria, Gemmatimonadales and unaffiliate group. Proteobacterium, Tetrasphaera elongate and Gemmatimonas aurantiaca may act important roles in phosphorus removal. With little amount as known glycogen accumulating organisms, Candidatus Competibacter phosphatis still at accumulating-phase had limited effect on microbial community structure. When climax community was obtained, dominant microbes were 14 OTUs. Microbes in a large amount were uncultured bacterium Thauera sp., uncultured y-Proteobacterium and Tetrasphaera elongata.

关键词： biological phosphorus removal polyphosphate accumulating organisms glycogen accumulating organisms

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Inhibitory factors affecting the process of enhanced biological phosphorus removal(EBPR)- a mini-review

Inhibitory factors affecting the process of enhanced biologi...

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浙江省环境科学学会2014年学术年会

作者： Xiongliu Zheng Peide Sun Jingyi Han Yingqi Song Zhirong Hu Haiqing Fan Shuyu Lv School of Environmental Science and Engineering Zhejiang Gongshang University Zhejiang metallurgical environmental protection design & research Co. LTD EnviroSim Associates Ltd Hamilton Ontario L8P 0A1 Canada

Enhanced biological phosphorus removal(EBPR) technology has been widely considered as a key strategy in preventing eutrophication and recognized as the advancing front of research in wastewater treatment. The key to keep its high efficiency in biological phosphorus removal is to optimize the operation and management of the system. Previous research in this field has undoubtedly improved understanding of the factors hindered overall efficiency of EBPR. However, it is obvious that much remains to be learnt. This paper attempts to review the fundamental understanding in factors inhibiting the stability and reliability of the EBPR systems in the state-of-the-art research. In view of modeling the EBPR systems, an appropriate extension of the current mechanistic models with these inhibitory factors is recommended in order to better simulate and predict the behavior of full-scale and lab-scale EBPR plants. From the perspectives of the further mechanistic and multi-factors study, the direction of denitrifying dephosphatation and granules/biofilms are also discussed. This comprehensive overview will not only help us to understand the overall mechanism of the EBPR process, but also benefit the researchers and engineers to consider all the possible factors affecting the process in the urban sewage treatment plants.

关键词： Enhanced biological phosphorus removal glycogen accumulating organisms Inhibitory factors Mechanism Modeling Polyphosphate accumulating organisms

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