检索结果-南通市图书馆

Effect of 2-Selenium Bridged β-Cyclodextrin,glutathione peroxidase Mimic on Stroke of Stroke-prone Spontaneously Hypertensive Rats

在线全文

学校读者我要写书评

暂无评论

Chemical Research in Chinese Universities 2004年第3期20卷 328-333页

作者： JIAZhi-dan SUNYe MUYing MAJi-sheng YANGang-lin LUOGui-min KeyLaboratoryforMolecularEnzymologyandEngineeringoftheMinistryofEducation lilinUniversityChangchun130023P.R.China JilinInstituteofNaturalMedicine Changchun130012P.R.China

To investigate the treatment effect of 2-selenium bridged β -cyclodextrin(2-SeCD),a GPX mimic,on the stroke of stroke-prone spontaneously hypertensive rats(SHRSP),fifty-two SHRSP of 8-week old were randomly divided into four groups A,B,C and control group D. The rats of groups A,B,C and D were given 1.0%-1.5% NaCl mass fraction as drinking fluid. After onset of stroke,groups A,B and C were given \{orally\} 16.05,160.5 and 1605 mg·kg -1 ·day -1 of 2-SeCD,respectively,and group D was given water for \{2 weeks.\} The clinical score of stroke,systolic blood pressure(SBP),survival time of rats were recorded and the histopathologic examinations of their brain and carotid artery were made after decapitation. The clinical scores of stroke after treatment with 160.5 mg·kg -1 ·day -1 (Group B) and 1605 mg·kg -1 ·day -1 (Group C) of 2-SeCD are 2.55±0.98 and 1.98±0.79,respectively,those are obviously lower than that of group D(3.41±0.83,p<0.01). The survival days in group B(10.0±8.6) and group C(14.4±7.9) are longer than that for group D(4.7±2.9,p<0.01). The electron microscope study showed that the endothelium of carotid artery was near to normal in group B and group C,while it was seriously injured in control group D and mildly injured in group A. 2-SeCD may effectively be used to treat the stroke for SHRSP.

关键词： 2-Selenium bridged β -cyclodextrin glutathione peroxidase Enzyme mimic Stroke Stroke-prone Spontaneously hypertensive rat

Synthesis and Characterization of Specific Haptens Used to Generate Abzyme with glutathione peroxidase Activity

在线全文

学校读者我要写书评

暂无评论

Chemical Research in Chinese Universities 1998年第3期14卷 55-57页

作者： Liu Jun-qiu Gao Shu-juan Luo Gui-min Shen Jia-cong The National Laboratory of Enzyme Engineering Key Laboratory for Supermolecular Spectra and Structure of Jilin UniversityJinlin UniversityChangchun130023

ＩｎｔｒｏｄｕｃｔｉｏｎＴｈｅｇｒｅａｔｐｒｏｇｒｅｓｓｉｎａｂｚｙｍｅｈａｓｂｅｅｎｍａｄｅｄｕｒｉｎｇｔｈｅｌａｓｔｄｅｃａｄｅ［１］．Ｔｈｅｋｅｙｔｏｔｈｅｐｒｅｐａｒａｔｉｏｎｏｆａｂｚｙｍｅｉｓｔｈｅｄｅｓ... 详细信息

ＩｎｔｒｏｄｕｃｔｉｏｎＴｈｅｇｒｅａｔｐｒｏｇｒｅｓｓｉｎａｂｚｙｍｅｈａｓｂｅｅｎｍａｄｅｄｕｒｉｎｇｔｈｅｌａｓｔｄｅｃａｄｅ［１］．Ｔｈｅｋｅｙｔｏｔｈｅｐｒｅｐａｒａｔｉｏｎｏｆａｂｚｙｍｅｉｓｔｈｅｄｅｓｉｇｎｏｆｈａｐｔｅｎ．Ｏｎｔｈｅ...

关键词： glutathione peroxidase Abzyme Hapten

Oxidative stress drives divergent evolution of the glutathione peroxidase(GPX)gene family in mammals

在线全文

学校读者我要写书评

暂无评论

Integrative Zoology 2021年第5期16卷 696-711页

作者： Ran TIAN Yuepan GENG Ying YANG Inge SEIM Guang YANG Jiangsu Key Laboratory for Biodiversity and Biotechnology College of Life SciencesNanjing Normal UniversityNanjingJiangsuChina Integrative Biology Laboratory College of Life SciencesNanjing Normal UniversityNanjingJiangsuChina School of Biology and Environmental Science Queensland University of TechnologyBrisbaneQueenslandAustralia

The molecular basis for adaptations to extreme environments can now be understood by interrogating the everincreasing number of sequenced *** such as cetaceans,bats,and highland species can protect themselves from oxidative stress,a disruption in the balance of reactive oxygen species,which results in oxidative injury and cell ***,we consider the evolution of the glutathione peroxidase(GPX)family of antioxidant enzymes by interrogating publicly available genome data from 70 mammalian species from all major *** identified 8 GPX subclasses ubiquitous to all mammalian *** GPX gene families resolved into the GPX4/7/8 and GPX1/2/3/5/6 groups and are characterized by several instances of gene duplication and loss,indicating a dynamic process of gene birth and death in *** of the eight GPX subfamilies(all but GPX7)were under positive selection,with the residues under selection located at or close to active sites or at the dimer *** also reveal evidence of a correlation between ecological niches(*** oxidative stress)and the divergent selection and gene copy number of GPX ***,a convergent expansion of GPX1 was observed in several independent lineages of mammals under oxidative stress and may be important for avoiding oxidative ***,this study suggests that the GPX gene family has shaped the adaption of mammals to stressful environments.

关键词： adaptive evolution gene family glutathione peroxidase mammals oxidative stress

Effects of low molecular weight heparin-superoxide dismutase conjugate on serum levels of nitric oxide,glutathione peroxidase,and myeloperoxidase in a gerbil model of cerebral ischemia/reperfusion injury

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Neural Regeneration Research 2008年第11期3卷 1233-1236页

作者： Qingde Wang Guixiang Cui Hongxia Liu Yizhao Li Fengshan Wang Department of Neurology Hospital of Shandong Aluminium Industry Co.Ltd. Zibo 255069 Shandong Province China Department of Rehabilitation Qilu Hospital Shandong University Jinan 250012 Shandong Province China Heze Third People＇s Hospital Heze 274000 Shandong Province China Department of Rehabilitation Shandong Disabled Rehabilitation Center Jinan 250100 Shandong Province China Institute of Biochemistry and Biotechnique College of Pharmacy Shandong University Jinan 250012 Shandong Province China

BACKGROUND： Several studies have demonstrated that low molecular weight heparin-superoxide dismutase （LMWH-SOD） conjugate may exhibit good neuroprotective effects on cerebral ischemia/reperfusion injury though anticoagulation, decreasing blood viscosity, having anti-inflammatory activity, and scavenging oxygen free radicals. OBJECTIVE： To investigate the intervention effects of LMWH-SOD conjugate on serum levels of nitric oxide （NO）, glutathione peroxidase （GSH-Px）, and myeloperoxidase （MPO） following cerebral ischemia/reperfusion injury. DESIGN, TIME AND SETTING： A randomized, controlled, and neurobiochemical experiment was performed at the Institute of Biochemical Pharmacy, School of Pharmaceutical Sciences, Shandong University between April and July 2004. MATERIALS： A total of 60 Mongolian gerbils of either gender were included in this study. Total cerebral ischemia/reperfusion injury was induced in 50 gerbils by occluding bilateral common carotid arteries. The remaining 10 gerbils received a sham-operation （sham-operated group）. Kits of SOD, NO, and MPO were sourced from Nanjing Jiancheng Bioengineering Institute, China. LMWH, SOD, and LMWH-SOD conjugates were provided by Institute of Biochemistry and Biotechnique, Shandong University, China. METHODS： Fifty successful gerbil models of total cerebral ischemia/reperfusion injury were evenly randomized to five groups： physiological saline, LMWH-SOD, SOD, LMWH ＋ SOD, and LMWH. At 2 minutes prior to ischemia, 0.5 mL/65 g physiological saline, 20 000 U/kg LMWH-SOD conjugate, 20 000 U/kg SOD, a mixture of SOD （20 000 U/kg） and LMWH （LMWH dose calculated according to weight ratio, LMWH： SOD = 23.6：51）, and LMWH （dose as in the LMWH ＋ SOD group） were administered through the femoral artery in each above-mentioned group, respectively. MAIN OUTCOME MEASURES： Serum levels of NO, MPO, and GSH-Px. RESULTS： Compared with 10 sham-operated gerbils, the cerebral ischemia/reperfusion injury gerbils exhibited decreased serum lev

关键词： cerebral ischemia/reperfusion nitric oxide myeloperoxidase glutathione peroxidase low molecular weight heparin-superoxide dismutase conjugate

Evaluation of glutathione peroxidase Enzymatic Activity in Seminal Plasma of Patients Treated at the Institute Pasteur in Cote d’Ivoire

在线全文

学校读者我要写书评

暂无评论

Advances in Reproductive Sciences 2023年第4期11卷 116-126页

作者： Marie Florence N’Guessan Bi Bali Sery Foua Jonas Vanié Bi N’Gbesso Amos Ekissi Youzan Ferdinand Djohan Founzégué Amadou Coulibaly Allico Joseph Djaman Biochemistry Unit Physiology of Reproductive Cells Institute Pasteur in Ivory Coast Abidjan Cote d’Ivoire The Laboratory of The Medical Sciences Training and Research Unit Felix Houphouet-Boigny University Abidjan Cote d’Ivoire Medical Sciences Training and Research Unit Department of Basic and Bioclinical Sciences Abidjan Felix Houphouet-Boigny Uni-versity Abidjan Cote d’Ivoire Laboratory of Biotechnology Agriculture and Valorization of Biological Resources Departement Biosciences University Félix Hou-phouet-Boigny Abidjan Cote d’Ivoire Agriculture Fishery Resources and Agro-Industry Training and Research Unit University of San-Pedro San-Pedro Cote d’Ivoire Departement of Clinical and Fundamental Biochemistry Institute Pasteur in Cote d’Ivoire Abidjan Cote d’Ivoire

glutathione peroxidase (GPx) is an antioxidant that plays an important role in the maintenance of male fertility. The aim of this study was to compare the profile of enzymatic activity of glutathione peroxidase in the seminal plasma of normozoosperm and those of pathological sperm. Thus, the activity of glutathione peroxidase was determined in the seminal plasma of 20 normozoosperms, 9 azoosperms and 31 oligoasthenoteratozoosperms. It was 37.58 ± 3.14 U/L in normozoosperms, 39.39 ± 2.27 U/L in oligoasthenoteratozoosperms, and 29.77 ± 2.62 U/L in azoosperms. The mean GPx enzyme activity of normozoosperms did not differ significantly from that of oligoasthenoteratozoosperms and azoosperms. In contrast, comparison of enzyme activity between abnormal sperms gave a significant difference. This study showed that glutathione peroxidase enzymatic activity is not related to sperm quality.

关键词： glutathione peroxidase Antioxidant Oligoasthenoteratozoospermia Azoospermia Normozoospermia

Synthesis and Kinetics of a Novel Mimic with glutathione peroxidase Activity—Tellurium-containing Hyaluronic Acid (TeHA)

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2006年第7期17卷 969-972页

作者： Zhi Bo CHEN Bo Xun ZHANG Zhong Xiu HUANG Qing Lin PENG Jia CHEN Yu WANG Jian Guo ZHANG Guang Zhi JIANG Wen Shu LI Lan Ying LIU College of Life Science Jilin University Changchun 130023 State Key Laboratory of Electroanalytical Chemistry Chinese Academy of SciencesChangchun Institute of Applied Chemistry Changchun 130022

A novel mimic was synthesized by modifying hyaluronic acid （HA） with tellurium, whose function is similar to that of glutathione peroxidase （GPX）. The structure of TeHA was characterized by means of IR and NMR, the target-Te was located at -CH2OH of the N-acetyl-D- glucosamine of HA. The H202 reducing activity of TeHA, by glutathione （GSH）, was 163.6 U/μmol according to Wilson＇s method. In contrast to other mimics, TeHA displayed the highest activity. Moreover, TeHA accepted many hydroperoxides as its substrates, such as H2O2, cumenyl hydroperoxide （CuOOH） and tert-butyl hydroperoxide （t-BuOOH）, and CuOOH was the optimal substrate of TeHA. A ping-pong mechanism was observed in the steady-state kinetic studies of the reactions catalyzed by TeHA.

关键词： Hyaluronic acid tellurium glutathione peroxidase synthesis kinetics.

Studies on Artificial Imitation of glutathione peroxidase by Chemical Mutation

在线全文

学校读者我要写书评

暂无评论

Chemical Research in Chinese Universities 1994年第2期10卷 131-133页

作者： DING Lan ZHU Zhen-qi LUO Gui-min SUN Qi-an YANG Tong-shu and SHEN Jia-cong(Laboratory of Enzyme Engineering, Jilin University , Changchun , 130023 )

fter the alcoholic group of serine located in the binding site of Anti-HumanIgM(Fcμ) (Rabbit IgG) was selectively activated by phenylmethylsulfonyl fluoride(PMSF) and displaced with hydrogen selenide(H2Se) , the serine residue was con-verted to selenocysteine(SeCys). Because SeCys is a catalytic group of glutathioneperoxidase (GPX), the mutated antibody has the GPX activity which is seventytimes more than that of PZ51, the best GPX mimic in the world. Moreover, themutated antibody remains its original antibody titer.

关键词： glutathione peroxidase Artificial enzyme Chemical mutation,Phenylmethyl sulfonyl fluoride Rabbit Anti-Human IgM(Fcμ)

glutathione peroxidase mimicry of diphenyl diselenide: Plausible contribution of proteins’ thiols

在线全文

学校读者我要写书评

暂无评论

Toxicology Advances 2023年第2期5卷 14-20页

作者： Ebenezer Morayo Ale Steve Osagie Asuelimen Olawale Otitoju Department of Biochemistry Faculty of Pure and Applied SciencesFederal University WukariTaraba State 670101Nigeria

Organoseleniums are a class of compounds attracting attention across the globe owing to their glutathione peroxidase(GPx)mimicry,which confers on them a strong antioxidant *** diselenide(DPDS)is an Organoselenium whose GPx mimetic property has been suggested to rely on the oxidation of non-protein or protein thiols critical to the activities of some sulfhydryl *** study,therefore investigated the GPx mimic/antioxidant property of DPDS as well as the role of thiols of two key sulfhydryl enzymes,cerebral Na^(+)/K^(+)-ATPase(sodium pump)and hepatic delta-aminolevulinic acid dehydratase(δ-ALAD)in the GPx mimicry of *** Wistar rats were euthanized,and the liver and brain were removed and used to assay for the effect of DPDS on lipid peroxidation induced by two prooxidants[Fe2^(+)(10μM)and H2O2,(1 mM)]as well as the activities of the sulfhydryl *** results revealed that DPDS profoundly(P<0.05)counteracted Fe2^(+)and H2O2-induced lipid peroxidation in the rats’hepatic and cerebral ***,the results of assay systems for lipid peroxidation and sodium pump revealed that DPDS inhibited Na^(+)/K^(+)-ATPase and lipid peroxidation in the brain tissue homogenates in the same reaction system.A similar result was obtained in the assay system for lipid peroxidation and hepaticδ-ALAD as DPDS simultaneously inhibited the enzyme’s activity and lipid *** suggests that the GPx mimetic property of DPDS may be linked to the enzymes’loss of activity,which further validates the suggestions that the enzymes’inhibition,as well as the antioxidant action of DPDS,rely on the oxidation of critical thiols of the ***,the GPx mimicry of DPDS should be investigated in the presence of thiol-blocking or oxidizing agents in biological systems in order to further ascertain the role of protein thiols.

关键词： Organoseleniums diphenyl diselenide glutathione peroxidase antioxidant thiols delta-aminolevulinic acid dehydratase Na^(+)/K^(+)-ATPase

Selenium-dependent glutathione peroxidases——A highlight of the role of phospholipid hydroperoxide glutathione peroxidase in protection against oxidative damage

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Progress in Natural Science:Materials International 2000年第5期 3-12页

作者：暴永平,WILLIAMSON Gary Diet Health and Consumer Sciences Division Institute of Food Research Norwich Research Park Norwich NR4 7UA UK Department of Nutrition and Food Hygiene Harbin Medical University Harbin 150001 China Department of Nutrition and Food Hygiene Harbin Medical University Harbin 150001 China

Since the discovery that selenium is an integral component of the active site of the mammalian glu-tathione peroxidase, four members of the glutathione peroxidase family have been characterised: classical cellular glu-tathione peroxidase, gastrointestinal glutathione peroxidase; plasma glutathione peroxidase and phospholipid hydroperoxide glutathione peroxidase (PHGPx) . They are products of different genes and have different specificities on hydrogen peroxide and lipid hydroperoxides, the latter are generated by free radicals and can damage cell membranes and disrupt cellular functions. Interestingly, PHGPx is not only active on phospholipid hydroperoxide, but also active on thymine hydroperoxide (a model compound for DNA damage) and protein hydroperoxides. This review highlights the role of PHGPx in protection against peroxidative damage of lipids, protein and DNA.

关键词： selenium selenoprotein phospholipid hydroperoxide glutathione peroxidase oxidative damage.