检索结果-南通市图书馆

Formalin-induced astrocyte glutamate-glutamine cycle involved in rat spinal cord central sensitization

Neural Regeneration Research 2009年第11期4卷 918-922页

作者： Haili Shen Yufeng XieO Hua Han Yayi Xia Youcheng Zhang Cuifang Wang Jing Wang Institute of Orthopaedics Second Hospital of Lanzhou University Lanzhou 730030 Gansu Province China Robarts Research Institute University of Western Ontario 100 Perth Drive London ON Canada N6A 5K8 Department of General Surgery Second Hospital of Lanzhou University Lanzhou 730030 Gansu Province China

BACKGROUND： Central sensitization, a state of increased excitability of nociceptive neurons in the spinal dorsal horn following peripheral tissue injury and/or inflammation, is an important mechanism underlying hyperalgesia and neuropathic pain. Participation of the glutamate-glutamine cycle in central sensitization of the spinal cord remains poorly understood. OBJECTIVE： To determine whether the astrocyte-neuronal glutamate-glutamine cycle is involved in formalin-induced central sensitization in the spinal cord. DESIGN, TIME AND SETTING： A randomized, controlled, animal experiment was performed at the Institute of Orthopedics, Second Hospital, Lanzhou University, China from September 2007 to August 2008. MATERIALS： Methionine sulfoximine （MSO, 0.1 mmol/L）, glutamine （0.25 mmol/L）, and formalin were used for this study. METHODS： A total of 43 male, Sprague Dawley rats, aged 4 months, were randomly assigned to a sham operation group （n = 6） and a model group （n = 37）. Rats in the model group received intrathecal infusion in the spinal cord. 7 days later, 37 model rats were randomly divided into PBS, MSO, glutamine, MSO ＋ glutamine and formalin subcutaneous injection alone groups. The PBS, MSO, glutamine, MSO ＋ glutamine groups were respectively intrathecally injected with PBS, MSO, glutamine, MSO ＋ glutamine （50 μL each）, and then infused with 10 μL of saline. Rats from the sham operation group were not subjected to intrathecal infusion in the spinal cord. At 15 minutes after intrathecal injection, a rat model of formalin-induced inflammatory pain was established by subcutaneous injection of 5% formalin （50 μL） in the left hindpaw. MAIN OUTCOME MEASURES： Changes in spontaneous nociceptive behavior （licking/biting or flinching） were observed following formalin injection into the rat hindpaw. RESULTS： Compared with the PBS group, duration of licking/biting was significantly shortened, and flinching frequency was significantly diminished in the MSO group （P 〈 0.05）. Com

关键词： astrocyte glutamate-glutamine cycle central sensitization inflammatory pain spinal cord

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Astroglial glutamate-glutamine cycle is involved in the modulation of inflammatory nociception in rats

引用

Neural Regeneration Research 2011年第35期6卷 2746-2750页

作者： Tiancheng Wang Jing Wang Bin Geng Hongyu Guo Haili Shen Yayi Xia Department of Neurology Second Hospital of Lanzhou University Lanzhou University Lanzhou 730030 Gansu Province China Institute of Orthopedics Second Hospital of Lanzhou University Lanzhou University Lanzhou 730030 Gansu Province China Department of Obstetrics and Gynecology Second Hospital of Lanzhou University Lanzhou University Lanzhou 730030 Gansu Province China Department of Pain Second Hospital of Lanzhou University Lanzhou University Lanzhou 730030 Gansu Province China

Our previous behavioral studies have indicated that the astroglial glutamate-giutamine cycle is involved in the process of formalin-induced spinal cord central sensitization, but there was little morphological evidence. In this study, double-labeling immunofluorescence techniques showed that after rats were intrathecally injected with PBS and plantarly injected with formalin, glial fibrillary acidic protein （GFAP） and glutamine synthesase （GS） expression were increased and GFAP/GS coexpression was changed to include layers III and IV. After intrathecal injection of methionine sulfoximine, a GS specific inhibitor, the formalin-induced change in expression and coexpression of GFAP and GS in spinal cord dorsal horns was inhibited. The morphology, distribution and quantity of astrocytes recovered to normal levels. An intrathecal glutamine injection reversed the inhibitory effect of methionine sulfoximine. Astrocytes showed significant activation and distribution extended to layers V and VI. The present study provides morphological evidence that the astroglial glutamateglutamine cycle is involved in the process of formalin-induced spinal cord central sensitization.

关键词： astrocyte glutamate-glutamine cycle central sensitization immunohistochemistry

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Ceftriaxone improves learning and memory deficits of APP/PS1 transgenic mice in early stage of Alzheimer’s disease

Ceftriaxone improves learning and memory deficits of APP/PS1...

引用

中国神经科学学会第十二届全国学术会议

作者： Shu-Juan Fan Xiao-Hui Xian Yu-Yan Hu Min Zhang Wen-Bin Li Department of Pathophysiology Hebei Medical University

Objective Glial glutamate transporter-1（GLT-1） is impaired in Alzheimer’s disease（AD）. Ceftriaxone（Cef） has been reported to up-regulate the expression and uptake activity of GLT-1. The present study was undertaken to explore whether Cef upregulate the expression of GLT-1 and proteins associated with glutamate-glutamine cycle and then alleviate the cognitive deficits in 6-and 7-month APP/PS1 mice. Methods The cognitive function, expression of GLT-1 and proteins related to glutamate-glutamine cycle and level of Aβ in hippocampus were assayed by Novel object recognition and Morris water maze tests, immunohistochemistry, western blot and ELISA, respectively. Results Cef treatment（200 mg/kg and 300 mg/kg） markedly improved cognitive deficits of APP/PS1 mice and up-regulated the expression of GLT-1 in hippocampus. Moreover, Cef up-regulated the activity of glutamine synthetase, the expression of system N glutamine transporter SN1, system A transporter GlnT and vesicular glutamate transporters, which were related to the glutamate-glutamine cycle. Conclusion Cef could improve cognitive deficits of APP/PS1 mice in early stage of AD by up-regulating GLT-1 expression and the effectiveness of glutamate-glutamine cycle.

关键词： Ceftriaxone APP/PS1 mice GLT-1 Morris water maze Alzheimer’s disease glutamate-glutamine cycle

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：