检索结果-南通市图书馆

Molecular cloning and functional characterization of an isoflavone glucosyltransferase from Pueraria thomsonii

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Natural Medicines 2022年第2期20卷 133-138页

作者： DUAN Hai-Yan WANG Jian ZHA Liang-Ping PENG Hua-Sheng ZHAO Yu-Ping YUAN Yuan HUANG Lu-Qi School of Pharmacy Anhui University of Chinese MedicineHefei 230012China State Key Laboratory Breeding Base of Dao-di Herbs China Academy of Chinese Medical SciencesBeijing 100700China Research Unit of DAO-DI Herbs Chinese Academy of Medical SciencesBeijing 100700China

Pueraria thomsonii has long been used in traditional Chinese *** are the principle pharmacologically active components,which are primarily observed as glycosyl-conjugates and accumulate in *** ***,the molecular mechanisms underlying the glycosylation processes in(iso)flavonoid biosynthesis have not been thoroughly *** the current study,an O-glucosyltransferase(PtUGT8)was identified in the medicinal plant *** from RNA-seq *** assays of the recombinant PtUGT8 showed that it was able to glycosylate chalcone(isoliquiritigenin)at the 4-OH position and glycosylate isoflavones(daidzein,formononetin,and genistein)at the 7-OH or 4′-OH position,exhibiting no enzyme activity to flavonones(liquiritigenin and narigenin)in *** identification of PtUGT8 may provide a useful enzyme catalyst for efficient biotransformation of isoflavones and other natural products for food or pharmacological applications.

关键词： Flavonone Isoflavone glucosyltransferase 4′-O-glucosylation 7-O-glucosylation

Cold Stress-induced glucosyltransferase CsUGT78A15 is Involved in the Formation of Eugenol Glucoside in Camellia sinensis

在线全文

学校读者我要写书评

暂无评论

Horticultural Plant Journal 2020年第6期6卷 439-449页

作者： Mingyue Zhao Binbin Cai Jieyang Jin Na Zhang Tingting Jing Jingming Wang Yuting Pan Zixiang Zhou Yifan Zhao Yingying Feng Feng Yu Mengting Zhang Yating Li Zhonghua Liu Chuankui Song State Key Laboratory of Tea Plant Biology and Utilization International Joint Laboratory on Tea Chemistry and Health EffectsAnhui Agricultural UniversityHefei 230036China Key Laboratory of Tea Science of Ministry of Education National Research Center of Engineering Technology for Utilization of Functional Ingredients from BotanicalsCollege of HorticultureHunan Agricultural UniversityChangsha 410128China

Eugenol is a natural phenolic compound known for its health-promoting properties and its ability to add a floral scent to tea *** eugenol glycosides have been identified and shown to make important contributions to fruit floral ***,the details of their biosynthesis and metabolism in tea plants are still ***,eugenol glucoside was unambiguously identified as a native metabolite in the tea plant,and its biosynthesis was shown to be induced by low temperature *** the analysis of UGTs induced by low temperature,the glycosyltransferase CsUGT78A15 was identified in tea,and its encoded protein was shown to catalyze the glucosylation of ***/Km ratios showed that eugenol was the most suitable substrate for *** donor preference analysis showed that CsUGT78A15 had a higher selectivity for glucose,followed by galactose and glucuronic *** expression of CsUGT78A15was correlatedwith the accumulation of eugenol glucoside in different tissues and genotypes of ***-regulation of CsUGT78A15 led to a decreased eugenol glucoside content under cold stress,indicating that CsUGT78A15 plays an important role in the biosynthesis of eugenol glucoside under cold *** identification of eugenol glucoside in the tea plant and the discovery of a cold stress-induced eugenol glucosyltransferase in tea provide the foundation for the improvement of tea flavor under cold stress and the biotechnological production of eugenol glucoside.

关键词： Camellia sinensis Eugenol glucosyltransferase Cold stress

Synthesis of Camptothecin-lO-O-glucoside Using an Engineered Oleandomycin glucosyltransferase

在线全文

学校读者我要写书评

暂无评论

Chemical Research in Chinese Universities 2018年第3期34卷 423-427页

作者： ZHU Xuelin YE Qinmei WEN Chao CHEN Liping LIANG Guangping ZENG Ting ZHANG Dongmei JIANG Renwang Guangdong Province Key Laboratory of Pharmacodynamic Constituents of Traditional Chinese Medicine and New Drugs Research Institute of Traditional Chinese Medicine and Natural Products College of Pharmacy Jinan University Guangzhou 510632 P. R. China

Oleandomycin glycosyltransferase variant P67T/S132F/A242V（ASP） was used to convert 10-hydroxycamptothecin into camptothecin-10-O-glucoside, which was confirmed by spectral analysis. Compared to the previously reported results, the present study reached the conversion rate up to 80% through the optimization of reaction conditions. In addition, compared with 10-hydroxycamptotheein（HCPT）, camptothecin-10-O-glucoside inhibited the proliferation of Huh7 cells in a concentration-dependent manner and showed stronger antineoplastic effect but lower toxicity. Furthermore, camptothecin-10-O-glucoside induced more apoptotic cells as compared with the parent compound.

关键词： Camptothecin-10-O-glucoside 10-Hydroxycamptothecin Glycosylation glucosyltransferase Antineoplastic

the Correlations between glucosyltransferase Activity and Deciduous Caries Susceptibility

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

the Correlations between Glucosyltransferase Activity and De...

Crocin Synthesis Mechanism in Crocus sativus

中华口腔医学会第14次全国口腔医学学术会议（2012年会）

作者： Ye Liang,Kewa Gao,Zeshuai Zeng,Xincheng Guo,Guoying Que~* Xiang-Ya school of Medicine,Central South University,Changsha,410078,China

Objectives:Dental caries is a kind of infectious disease,which is found prevalent among 70%children in *** caries is subject to *** some children have high dmft(decay miss and fill total) while some have low or none,s... 详细信息

关键词： Caries Susceptibility glucosyltransferase

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Tsinghua Science and Technology 2005年第5期10卷 567-572页

作者：杨波郭志刚刘瑞芝 Department of Chemical Engineering Tsinghua University Beijing 100084 China

Saffron （Crocus sativus） cells can synthesize crocin, crocetin digentiobiosyl ester, in suspension cultures. The crocin family biosynthesis mechanism was studied using high pressure liquid chromatography （HPLC） to determinate the glucosyltransferase activity and to develop a method for synthesizing medicine from saffron cells. Previous studies indicated that two glucosyltransferases might be involved in the formation of crocetin glucosyl- and gentiobiosyl-esters. GTasel formed an ester bond between crocetin carboxyl groups and glucose moieties while GTase2 catalyzed the formation of glucosidic bonds with glucosyl ester groups at both ends of the molecule. These enzymes can catalyze the formation of crocetin glucosides in vitro. GTasel activity is higher during the first four days of crocin glucosides biosynthesis, but decreases after four days. The formation and accumulation of crocin increase during the first six days and stabilized on the eighth day.

关键词： saffron crocetin crocetin di-neapolitanosyl ester glucosyltransferase glycosylation

Biofilm Formation by <i>Streptococcus mutans</i>and Related Bacteria

在线全文

学校读者我要写书评

暂无评论

Advances in Microbiology 2012年第3期2卷 208-215页

作者： Junko Nishimura Tadao Saito Hiroshi Yoneyama Lan Lan Bai Kazuhiko Okumura Emiko Isogai Department of Oral Surgery Health Sciences University of Hokkaido Hokkaido Japan Laboratory of Animal Microbiology Graduate School of Agricultural Science Tohoku University Sendai Japan Laboratory of Animal Products Chemistry Graduate School of Agricultural Science Tohoku University Sendai Japan

Caries is a disease of human dentition characterized by the loss of mineralized surfaces of the tooth;it is an infectious disease of the oral cavity in which biofilms play a causative role. Control of biofilms has traditionally relied on non-specific removal of plaque by mechanical means such as brushing, although it is difficult to remove biofilms by this method. Caries is also a widespread infection in children. Streptococcus mutans and S. sobrinus are important causative agents of caries. They produce a homologous exocellular polysaccharide called glucan, which strongly adheres to the enamel surface. This is a review of oral microbial biofilm formation by S. mutans and other related bacteria.

关键词： Caries Streptococcus mutans Streptococcus sobrinus Biofilm Exocellular Polysaccharide Glucan glucosyltransferase

UGT85A53 catalyzes the glucosylation of (Z)-3-hexenol,an important info chemical in interspecies interactions in Camellia sinensis

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

UGT85A53 catalyzes the glucosylation of (Z)-3-hexenol,an imp...