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Journal of Central South University of Technology 2007年第1期14卷 73-77页

作者：魏万之翟秀荣曾金祥高艳萍龚淑果 State key Laboratory of Chemo-Biosensing and Chemometrics School of Chemistry and Chemical EngineeringHunan University Department of Chemistry Jining Teachers College

A new nanocomposite material for construction of glucose biosensor was prepared. The biosensor was formed by entrapping glucose oxidase(Gox) into chitosan/nanoporous ZrO2/multiwalled carbon nanotubes nanocomposite film. In this biosensing thin film, the multiwalled carbon nanotubes can effectively catalyze hydrogen peroxide and nanoporous ZrO2 can enhance the stability of the immobilized enzyme. The resulting biosensor provides a very effective matrix for the immobilization of glucose oxidase and exhibits a wide linear response range from 8 μmol/L to 3 mmol/L with a correlation coefficient of 0.994 for the detection of glucose. And the response time and detection limit of the biosensor are determined to be 6 s and 3.5 μmol/L, respectively. Another attractive characteristic is that the biosensor is inexpensive, stable and reliable.

关键词： biosensor nanocomposite glucose oxidase nanoporous ZrO2 multiwalled carbon nanotubes chitosan

Predominating stable adsorption and direct electrochemistry of glucose oxidase on carbon nanotubes by oxygen-containing groups

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Chinese Chemical Letters 2007年第3期18卷 313-315页

作者： Chun Hai Yang Cheng Guo Hu Sheng Shui Hu Departraent of Chemistry Wuhan University Wuhan 430072 China Key Laboratory of Biological Resources Protection and Utilization of Hubei Province Enshi 445000 China

Stable adsorption and direct electrochemistry of glucose oxidase （COx） occurred on nitric acid （HNO3）-treated multi-walled carbon nanotubcs （MWNTs） instead of as-received MWNTs, demonstrating the critical roles ... 详细信息

关键词： glucose oxidase Adsorption Direct electrochemistry Carbon nanotubes Oxygen-containing groups

Direct Electron Transfer between glucose oxidase and Multi-walled Carbon Nanotubes

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Chinese Journal of Chemistry 2003年第8期21卷 1088-1091页

作者：徐锦忠朱俊杰吴强胡征陈洪渊 Laboratory of Mesoscopic Materials Science Department of ChemistryNanjing University

The direct electrochemical behavior between the glucose oxidase (GOD) and the multi walled carbon nanotubes (MWNTs) has been studied. Two pairs of cyclic voltammetric peaks corresponding to the two different processes, i.e. mass transport and surface reaction of GOD are observed on this MWNTs. The formal potentials with E o′=-0.45 V and E o′=-0.55 V were obtained respectively. The GOD film was observed on the carbon nanotube by the TEM.

关键词： carbon nanotube glucose oxidase direct electron transfer

The Amperometric glucose Enzyme Electrode by Immobilized glucose oxidase in glucose oxidase/N-ethyl-Poly (4-vinyl)pyridine Multilayer

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Chinese Chemical Letters 1998年第11期9卷 0-0,0页

作者： Ma, YZ Heze Teachers Coll Dept Chem Shandong 274015 Peoples R China

Multilayers of glucose oxidase(GOD)/N-ethyl-poly (4-vinyl) pyridine (EPVP) have been assembled on thiol self-assembled monolayers on gold *** electrode can be used as an amperometric enzyme elcctrode for glucose. Fe (CN)63-/4- incorporated in EPVP layer acts as the electron mediator, the linear range was 0.1 to 6 mmol/L when the number of GOD layers was 5.

关键词： Enzyme electrode glucose oxidase N-ethyl-poly (4-vinyl) pyridine. multilayer.

A glucose Biosensor Based on Immobilization of glucose oxidase in Chitosan Network Matrix

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Chinese Journal of Chemistry 2005年第3期23卷 275-279页

作者：姚慧李楠徐锦忠朱俊杰 KeyLaboratoryofAnalyticalChemistryforLifeScience DepartmentofChemistryNanjingUniversityNanjingJiangsu210093China

A simple and promising glucose biosensor was constructed by successful entrapment of glucose oxidase (GOD) into chitosan matrix, which was cross‐linked with glutaraldehyde (GA). This material provided good biocompatibility and the stabilizing microenvironment around the enzyme. The morphologies and properties of chitosan and chitosan/GOD were characterized by FTIR, UV‐Vis and SEM techniques. This biosensor exhibited a fast amperometric response to glucose. The linear range for glucose determination was from 1×10 −5 to 3.4×10 −3 mol·L −1 , with a detection limit of 5×10 −6 mol·L −1 based on S/N =3. The biosensor could retaCin ca. 90% of its original activity after two weeks of storage under dry conditions at 4 °C.

关键词： chitosan glucose oxidase biosensor, glucose crosslink

Enhanced thermostability and catalytic efciency of glucose oxidase in Pichia Pastoris

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Systems Microbiology and Biomanufacturing 2022年第2期2卷 296-304页

作者： Hanlei Zhou Pu Zheng Pengcheng Chen Xiaowei Yu Dan Wu The Key Laboratory of Industrial Biotechnology Ministry of EducationSchool of BiotechnologyJiangnan UniversityWuxi 214122China

glucose oxidase(GOD)has many practical applications,but its poor thermostability limits its broader *** this research,three primary mutants of wild-type GOD were designed using rational mutagenesis,and the GODm mutant was constructed by combinatorial *** expression,purifcation,and enzymatic properties of the mutants were *** specifc enzyme activity of GODm was 2.10-fold higher than that of wild type,and the(k_(cat)/K_(m))value was increased by *** treatment at 55℃for 3 h,GODm retained 37.5%of its enzymatic activity,and the half-life(t_(1/2))of GODm at 55℃and 65℃was 2.28-fold and 3.36-fold higher than that of wild type,*** analyzing the three-dimensional structure of wild type and the GODm mutant,it was found that T30V formed a new hydrogen bond with FAD and strengthened the hydrophobic interaction,D315K optimized the surface electrostatic interaction,and A162T improved the efciency of the electron ***,a novel mutant with improved thermostability and catalytic efciency was obtained in this research.

关键词： glucose oxidase Pichia pastoris Thermostability Catalytic efciency Rational mutagenesis

A Chemiluminescence Optical Fiber glucose Biosensor Based on Co-immobilizing glucose oxidase and Horseradish Peroxidase in a Sol-gel Film

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Chemical Research in Chinese Universities 2002年第1期18卷 12-15页

作者： LI Ying-xiu, ZHU Lian-de and ZHU Guo-yi State Key Lab of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P.R. ChinaZHAO Chun College of Chemistry, Jilin University, Changchun 130023, P Changchun Institute of Applied Chemistry Chinese Academy of Sciences State Key Lab of Electroanalytical Chemistry Changchun 130022 College of Chemistry Jilin University Changchun 130023

An optical fiber bienzyme sensor based on the luminol chemiluminescent reaction was developed and demonstrated to be sensitive to glucose. glucose oxidase(GOD) and horseradish peroxidase(HRP) were co-immobilized by microencapsulation in a sol-gel film derived from tetraethyl orthosilicate(TEOS). The calibration plots for glucose were established by the optical fiber glucose sensor fabricated by attaching the bienzyme silica gel onto the glass window of the fiber bundle. The linear range was 0 2-2 mmol/L and the detection limit was approximately 0 12 mmol/L. The relative standard deviation was 5.3% ( n =6). The proposed biosensor was applied to glucose assay in ofloxacin injection successfully.

关键词： Fiber optical biosensor Sol-gel Chemiluminescence glucose oxidase Horseradish peroxidase glucose assay

A glucose biosensor based on direct electrochemistry of glucose oxidase immobilized onto platinum nanoparticles modified graphene electrode

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Science China(Physics,Mechanics & Astronomy) 2012年第7期55卷 1163-1167页

作者： LIU AiRong HUANG ShiMing State Key Laboratory of Pollutton Control and Resources Reuse College of Environmental Science and EngineeringTongji UniversityShanghai 200092China Department of Physics Tongji UniversityShanghai 200092China

The platinum nanoparticles were adsorbed on graphene oxide sheets and played an important role in catalytic reduction of graphene oxide with hydrazine, leading to the formation of graphene-Pt nanoparticles. Because of their good electronic properties, biocompatibility and high surface area, graphene-Pt based composites achieved the direct electron transfer of redox enzyme and maintained their bioactivity well. The graphene-Pt nanocomposites were characterized using X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), high-resolution transmission electron microscopy (HR-TEM) and selected area electron diffraction (SAED). The amperometric biosensor fabricated by depositing glucose oxidase over Nafion-solubilized graphene-Pt electrode retained its biocatalytic activity and has offered fast and sensitive glucose quantification.

关键词： platinum nanoparticle glucose oxidase graphene biosensor

Light-triggered plasmonic vesicles with enhanced catalytic activity of glucose oxidase for programmable photothermal/starvation therapy

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Science China Materials 2021年第5期64卷 1291-1301页

作者： Zhuoting Deng Yaling He Muhammad Rizwan Younis Shan Lei Chao Jiang Yanyan Yuan Peng Huang Jing Lin Marshall Laboratory of Biomedical Engineering International Cancer CenterLaboratory of Evolutionary Theranostics(LET)School of Biomedical EngineeringShenzhen University Health Science CenterShenzhen 518060China

glucose oxidase(GOx)-based nanotheranostic agents hold great promise in tumor starvation and its synergistic therapy. Self-assembled plasmonic gold vesicles(GVs) with unique optical properties, large hollow cavity, and strong localized surface plasmon resonance, can be used as multi-functional nanocarriers for synergistic therapy. Herein,GOx-loaded GVs(GV-GOx) were developed for light-triggered GOx release as well as enhanced catalytic activity of GOx, achieving programmable photothermal/starvation therapy. Under near-infrared laser irradiation, the GV-GOx generated strong localized hyperthermia due to plasmon coupling effect of GVs, promoting the release of encapsulated GOx and increasing its catalytic activity, resulting in enhanced tumor starvation effect. In addition, the high photothermal effect improved the cellular uptake of GV-GOx and allowed an efficient monitoring of synergistic tumor treatment via photoacoustic/photothermal duplex imaging in vivo. Impressively, the synergistic photothermal/starvation therapy demonstrated complete tumor eradication in 4 T1 tumorbearing mice, verifying superior synergistic anti-tumor therapeutic effects than monotherapy with no apparent systemic side effects. Our work demonstrated the development of a light-triggered nanoplatform for cancer synergistic therapy.

关键词： light-triggered release plasmonic vesicles glucose oxidase photothermal therapy starvation therapy

Direct Electron Transfer Reactions of glucose oxidase and D-Amino Acid oxidase at a Glassy Carbon Electrode in Organic Media

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Advances in Manufacturing 1998年第1期 80-83页

作者： Wei Jianjun Qin Yiqin Liu Haiying(School of Chemistry and Chemical Engineering) Deng Jiaqi(Fudan University)

Cyclic voltammetry is employed to demonstrate feasibility of direct electron transfer of glucose oxidase and D amino acid oxidase at a glassy carbon electrode in organic media. The reversible slight conformational change of glucose oxidase is observed by changing 0.1 mol/L phosphate buffer to acetonitrile containing 10% v/v of water and 0.05 mol/L tetrabutyalammonium perchlorate, and vice versa.

关键词： glucose oxidase D amino acid oxidase direct electron transfer