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Effect of ginsenoside rbl on Expressive Proteome of Rat Neurons

Agricultural Biotechnology 2012年第2期1卷 17-19页

作者： Chunyan ZHANG Qian XU Chunling ZHAO Chunlai ZHANG Department of Biochemistry Luzhou Medical College Department of Physiology Luzhou Medical College

[ Objective ] This study aimed to explore the effect of ginsenoside rbl on expressive proteome of rat neurons by technologies of proteomics, bioinformat- ies and MS peptide fingerprinting. [ Method ] Rat neurons were cultured conventionally and randomly separated into two groups. The experimental group was trea- ted with 5 μg/rnl rbl for 20 min, while control group was added with the same amount of medium. After cell lysis, the whole-cell protein was extracted. Two-di- mensional electrophoresis （2-DE） was used to separate the extracts. Differential expression of proteome between the two groups was analyzed by using ImageMaster 2D Platinum v5.0 software and the two protein spots expressed differently were selected for differential identification with MALDI-TOF-MS. [ Result] Based on the matching and comparative analysis of the protein spots, 418 protein spots were detected in experimental group, including 226 protein spots with differently expres- sive levels; according to the mass spectrometry, the two ginsenoside rbl-related and differentially-expressed protein spots were identified as cytochrome P-450 and phosducin-like protein, and both of them were phosphorylated proteins. [ Conclusion ] This study showed that the functions of those identified proteins were in- volved in signal transduction, suggesting that the effect of ginsenoside rbl on expressive proteome of rat neurons might be related to the corresponding signal trans- duction networks.

关键词： ginsenoside rbl Rat neurons Expressive proteome Bioinformatics

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Effects of ginsenoside Rb1 Babu Agent on the Amplitude of Microvascular Vasomotion

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Medicinal Plant 2018年第2期9卷 78-82页

作者： Yuxin ZHU Yang GAO Qian ZHANG Tao ZHANG Wenyu ZHU Bo FENG Jianfang WANG Hong DONG Xiang MU College of Animal Science and Technology Beijing University of AgricultureBeijing Key Laboratory of Traditional Chinese Veterinary Medicine Beijing Huairou District Bureau of Agriculture College of Veterinary Medicine China Agricultural University

[Objectives] To study the effects of ginsenoside rbl Babu agent on the amplitude of microvascular vasomotion. [Methods]The in vitro transdermal permeation of ginsenoside rbl Babu agent was performed by using intelligent transdermal apparatus,and the percutaneous fluid was collected and analyzed by using high performance liquid chromatography( HPLC). The Babu agent was stuck on the skin of acupoint area,determined by Laser Doppler Flowmeter,and amplitude of microvascular vasomotion of acupoint area was recorded. [Results] With the extension of transdermal time,the cumulative permeation rate of ginsenoside rbl increased. The amplitude of microvascular vasomotion could be significantly increased with the application of ginsenoside rbl Babu agent( P ginsenoside rbl Babu agent can release the drug into the acupoint,increase the amplitude of microvascular vasomotion,and achieve the effect of acupuncture. Therefore,ginsenoside rbl Babu agent can replace the acupuncture clinically to treat diseases.

关键词： ginsenoside rbl Babu agent Microvessel Vasomotion Amplitude

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