Lignin is one of the main components of cell walls and is essential for resistance to insect pests in *** plants are damaged by aphid(Aphis gossypii) worldwide but resistant breeding is undeveloped due to scarce knowl...
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Lignin is one of the main components of cell walls and is essential for resistance to insect pests in *** plants are damaged by aphid(Aphis gossypii) worldwide but resistant breeding is undeveloped due to scarce knowledge on resistance genes and the mechanism. This study reported a lignin biosynthesisrelated gene identified in the F_(2) population derived from the cross between cotton cultivars Xinluzao 61(resistant to aphid) and Xinluzao 50(susceptible to aphid). A quantitative trait locus was mapped on chromosome D04 with a logarithm of odds(LOD) score of 5.99 and phenotypic effect of 27%. RNA-seq analysis of candidate intervals showed that the expression level of GH_D04G1418 was higher in the resistant cultivar than in the susceptible cultivar. This locus is close to AtLAC4 in the phylogenetic tree and contains a conserved laccase domain. Hence, it was designated ghlac4-3. Silencing of ghlac4-3 in Xinluzao 61 via virus-induced gene silencing(VIGS) resulted in decreased lignin content and increased susceptibility to aphids. These results suggest that ghlac4-3 might enhance aphid resistance by regulating lignin biosynthesis in cotton.
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