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Resolving the geometric structure of trastuzumab by mobility capillary electrophoresis and native mass spectrometry

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Chinese Chemical Letters 2024年第3期35卷 221-225页

作者： Wenjing Zhang Jie Hong Lei Yang Zuqiang Xu Yu Xiang Wei Xu School of Life Science Beijing Institute of TechnologyBeijing 100081China

Available online Immunoglobulins G(IgGs)are Y-shaped globular proteins,however,their high flexibility and heterogeneity pose great challenges to their structure and conformation *** structure of IgG closely correlates to its biofunctions,such as the antibody escape of human immunodeficiency virus(HIV)could attribute to the distance mismatch between the ends of two Fab arms(antigen-binding sites)and envelope glycoprotein spikes on virion ***,we report the first use of mobility capillary electrophoresis(MCE)and native mass spectrometry(nMS)to resolve the internal geometric structure and conformation of an IgG(trastuzumab)in solution *** proteolysis,the ellipsoid dimensions of IgG and its subunits were measured by MCE-nMS *** was then reconstructed,in which the sizes and relative positions of these three subunits in three-dimensional space were *** was found that the two Fab arms have an angle of~102.1°and a distance of~11.0 nm between the two antigen-binding sites under native condition,and the Fc arm was tilted~16.0°towards one of the Fab *** was not on the plane of Fab-Fab,but has an angle of no larger than 103.1°.Under acidic environment(pH 3.0),each subunit of the IgG would unfold into larger dimensions,and the angles between these subunits also *** great potential for tumor imaging and therapy,the structure of F(ab')_(2)fragments was also measured and validated by molecular dynamic *** was found that the electrostatic force among these three subunits and steric hindrance stemming from Fc help maintaining the angle between two Fab arms.

关键词： Native mass spectrometry Mobility capillary electrophoresis Trastuzumab Protein structure geometric structure

Analytic approach on geometric structure of invariant manifolds of the collinear Lagrange points

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Science China(Physics,Mechanics & Astronomy) 2012年第9期55卷 1703-1712页

作者： LU Jing WANG Qi WANG ShiMin School of Aeronautics Science and Engineering Beihang University Beijing 100191 China

An analytical method is proposed to find geometric structures of stable,unstable and center manifolds of the collinear Lagrange *** a transformed space,where the linearized equations are in Jordan canonical form,these invariant manifolds can be approximated arbitrarily closely as Taylor series around Lagrange *** invariant manifolds are represented by algebraic equations containing the state variables only without the help of *** the so-called geometric structure of these invariant manifolds is *** stable,unstable and center manifolds are tangent to the stable,unstable and center eigenspaces,*** an example of applicability,the invariant manifolds of L 1 point of the Sun-Earth system are *** stable and unstable manifolds are symmetric about the line from the Sun to the Earth,and they both reach near the Earth,so that the low energy transfer trajectory can be found based on the stable and unstable *** periodic or quasi-periodic orbits,which are chosen as nominal arrival orbits,can be obtained based on the center manifold.

关键词： analytical method stable manifold unstable manifold center manifold geometric structure

A Density Functional Theory Study of the geometric and Electronic structure of MgF_2 (110) Surface

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Journal of Wuhan University of Technology(Materials Science) 2013年第1期28卷 22-25页

作者：王丽平 HAN Peide ZHANG Caili HAO Yuying 许并社 Key Laboratory of Interface Science and Engineering in Advanced Materials of Taiyuai University of Technology Department of Physics and Optelectronics Taiyuan University of Technology College of Materials Science and Engineering Taiyuan University of Technology

Abstract＂ Ab initio density functional theory （DFT） was employed to study geometric and electronic structure of MgF2 （110） surface. Three different clean surface models have been considered. The results show that the surface terminated with one-layer F has the smallest relaxation and the lowest surface energy, which indicates this model is the most energetically favorable structure of MgF2（110） surface. Furthermore, the electronic properties are also discussed from the point of density of states and charge density. Analysis of electronic structure shows that the band gap of the surface is significantly narrowed with respect to the bulk. The electrons of the surface exhibit strong locality and larger effective mass.

关键词： geometric structure electronic structure stability surface energy first principles

From concept to commercialization:A review of tubular solid oxide fuel cell technology

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Journal of Energy Chemistry 2024年第10期97卷 79-109页

作者： Ruyan Chen Yuan Gao Jiutao Gao Huiyu Zhang Martin Motola Muhammad Bilal Hanif Cheng-Xin Li State Key Laboratory for Mechanical Behavior of Materials School of Materials Science and Engineering Xi'an Jiaotong University Department of Inorganic Chemistry Faculty of Natural Sciences Comenius University Bratislava

The reduced sealing difficulty of tubular solid oxide fuel cells(SOFCs) makes the stacking of tubular cell groups relatively easy,and the thermal stress constraints during stack operation are smaller,which helps the stack to operate stably for a long *** special design of tubular SOFC structures can completely solve the problem of high-temperature sealing,especially in the design of multiple single-cell series integrated into one tube,where each cell tube is equivalent to a small electric stack,with unique characteristics of high voltage and low current output,which can significantly reduce the ohmic polarization loss of tubular *** paper provides an overview of typical tubular SOFC structural designs both domestically and *** on the geometric structure of tubular SOFCs,they can be divided into bamboo tubes,bamboo flat tubes,single-section tubes,and single-section flat tube ***,this article provides an overview of commonly used materials and preparation methods for tubular SOFCs,including commonly used materials and preparation methods for support and functional layers,as well as a comparison of commonly used preparation methods for microtubule SOFCs,It introduced the three most important parts of building a fuel cell stack:manifold,current collector,and ceramic adhesive,and also provided a detailed introduction to the power generation systems of different tubular SOFCs,Finally,the development prospects of tubular SOFCs were discussed.

关键词： geometric structure Preparation methods Stack Support material Tubular solid oxide fuel cell

geometric structures of Stable Time-Variant State Feedback Systems

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Journal of Beijing Institute of Technology 2007年第4期16卷 500-504页

作者：仲锋惟孙华飞张真宁 School of Science Beijing Institute of Technology

A new technique for considering the stabilizing time-variant state feedback gains is proposed from the viewpoint of information geometry. First, parametrization of the set of all stabilizing time-variant state feedback gains is given. Moreover, a diffeomorphic structure between the set of stabilizing time-variant state feedback gains and the Cartesian product of positive definite matrix and skew symmetric matrix satisfying certain algebraic conditions is constructed. Furthermore, an immersion and some results about the eigenvalue locations of stable state feedback systems are derived.

关键词： geometric structure state feedback immersion diffeomorphism

An Original Didactic of the Standard Model “The Particle’s geometric Model” (Nucleons and K-Mesons)

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Journal of High Energy Physics, Gravitation and Cosmology 2024年第3期10卷 1054-1078页

作者： Giovanni Guido Abele Bianchi Gianluigi Filippelli External Contact Referent of the EEE Project at the High Scholl C. Cavalleri Parabiago Italy External Contact Referent of the Extreme Energy Events (EEE) Project at the High Scholls Gandini Lodi Italy Astronomic Observatory Brera Milan Italy

This paper shows a didactic model (PGM), and not only, but representative of the Hadrons described in the Standard Model (SM). In this model, particles are represented by structures corresponding to geometric shapes of coupled quantum oscillators (IQuO). By the properties of IQuO one can define the electric charge and that of color of quarks. Showing the “aurea” (golden) triangular shape of all quarks, we manage to represent the geometric combinations of the nucleons, light mesons, and K-mesons. By the geometric shape of W-bosons, we represent the weak decay of pions and charged Kaons and neutral, highlighting in geometric terms the possibilities of decay in two and three pions of neutral Kaon and the transition to anti-Kaon. In conclusion, from this didactic representation, an in-depth and exhaustive phenomenology of hadrons emerges, which even manages to resolve some problematic aspects of the SM.

关键词： Golden Particle Quark Sub-Oscillator Semi-Quanta IQuO geometric structure Golden Number Massive Coupling Interpenetration Nucleon Kaon Boson

Research Progress of Fabrics with Different geometric structures for Triboelectric Nanogenerators in Flexible and Wearable Electronics

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Advanced Fiber Materials 2023年第6期5卷 1852-1878页

作者： Dali Yan Jian Ye Yahui Zhou Xingxin Lei Bo Deng Weilin Xu State Key Laboratory of New Textile Materials and Advanced Processing Technologies Wuhan Textile UniversityWuhan 430200China

Widespread reliance on fossil fuels,and the resulting imbalance between energy supply and demand have emerged as significant obstacles to achieving sustainable *** nanogenerators(TENGs)offer a viable solution to this *** the various materials used in TENGs,fabrics with geometric structures have attracted considerable interest because of their advantageous properties,such as their light weight,breathable structures,favorable softness,and excellent *** review provides a comprehensive introduction to fabric geometric(fabric structure with yarn as the basic unit,including woven fabrics formed by warp and weft yarns and knitted fabrics formed by yarn coils,etc.)TENGs,including their definition,working principle,and mechanisms,and explores the recent progress in TENGs based on one-,two-,and three-dimensional structures,classifying them into woven and knitted fabrics according to the fabrication *** summarize the advantages and disadvantages of TENGs with different *** the intrinsically limited conductivity of the fiber and fabric,progress in improving the comprehensive output performance of TENGs via combination with other conductive materials and surface modification is ***,this review concludes with a discussion of the challenges,opportunities,and potential applications related to TENGs based on fabric geometric *** study is expected to provide readers with new strategies and conceptual ideas to improve the performance of TENGs constructed with fabrics,particularly through the optimization of their structures.

关键词： geometric structure Triboelectric nanogenerators Dimensional Fabric-based

Information geometric structures for the Thermodynamic Manifold

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Journal of Beijing Institute of Technology 2010年第4期19卷 491-494页

作者：张士诚孙华飞李春辉 School of Science Beijing Institute of Technology Beijing 100051 China School of Mathematics Xuzhou Normal University Xuzhou Jiangsu 221116 China

In view of information geometry,the state space S of thermodynamic parameters is *** a Riemannian metric for S is defined and then the α-geometric structures of S is *** of results obtained by other authors are exten... 详细信息

关键词： thermodynamic manifold information geometry geometric structure

Oxidation of formic acid on stepped Au(997) surface

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Chinese Journal of Catalysis 2016年第10期37卷 1738-1746页

作者：邬宗芳姜志全金岳康熊锋孙光辉黄伟新中国科学技术大学微尺度物质科学国家实验室(筹) 能量转换材料中国科学院重点实验室和化学物理系安徽合肥230026

The adsorption and reaction of formic acid （HCOOH） on clean and atomic oxygen‐covered Au（997） surfaces were studied by temperature‐programmed desorption/reaction spectroscopy （TPRS） and X‐ray photoelectron spectroscopy （XPS）. At 105 K, HCOOH molecularly adsorbs on clean Au（997） and interacts more strongly with low‐coordinated Au atoms at （111） step sites than with those at （111） terrace sites. On an atomic oxygen‐covered Au（997） surface, HCOOH reacts with oxygen at‐oms to form HCOO and OH at 105 K. Upon subsequent heating, surface reactions occur among ad‐sorbed HCOO, OH, and atomic oxygen and produce CO2, H2O, and HCOOH between 250 and 400 K. The Au（111） steps bind surface adsorbates more strongly than the Au（111） terraces and exhibit larger barriers for HCOO（a） oxidation reactions. The surface reactions also depend on the relative coverages of co‐existing surface species. Our results elucidate the elementary surface reactions between formic acid and oxygen adatoms on Au surfaces and highlight the effects of the coordina‐tion number of the Au atoms on the Au catalysis.

关键词： Gold catalysis Surface chemistry Model catalyst geometric structure Coordination number