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Development and Validation of an Indirect Whole-virus ELISA Using a Predominant genotype vi Velogenic NewCastle Disease virus Isolated from Lebanese Poultry

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Open Journal of Veterinary Medicine 2023年第6期13卷 82-95页

作者： Houssam Shaib Hasan Hussaini Roni Sleiman Youmna Iskandarani Youssef Obeid Faculty of Agricultural and Food Sciences American University of Beirut Beirut Lebanon

Commercial ELISA kits are commonly used to assess the levels of chicken antibodies against NewCastle Disease virus (NDV) and trace a field strain infection. Nevertheless, the specificity of these kits vis-à-vis endemic strains in Lebanon remains in question. This study developed an in-house indirect ELISA system to evaluate the level of chicken antibodies against a predominant velogenic NDV strain belonging to genotype vi. A checkerboard analysis comprised a five-factorial multivariate experiment to optimize the protocol: coating antigen concentration, blocking buffer utilization, serum and conjugate dilution levels, and OD reading wavelength. The developed test was optimized and then validated through parallel testing of the sera of 20 broilers and 5 layers using standard serological assays. There was a strong correlation between the developed ELISA results and those obtained with the Hemagglutination Inhibition test (P vity, and reproducibility of the developed ELISA suggest that it can be used as the test of choice for the assessment of chicken antibody titers against locally circulating velogenic NDV strains, specifically those belonging to genotype vi. It also offers better help in the serological detection of birds’ exposure to the said strains.

关键词： ELISA Development genotype vi NewCastle Disease Poultry Lebanon

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Comparisons of the Pathogenicity between Pigeon-derived and Chickenderived genotype Ⅵ Newcastle Disease virus(NDV) Strains in Pigeons

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Agricultural Biotechnology 2016年第1期5卷 40-43页

作者： XingangHU Shuang WU Yinyun HUANG Guangfu GUO Jiangsu Agri-animal Husbandry Vocational College Taizhou 225300China Center for Epidemiologic Studies of Animals in Jiangsu Province Taizhou 225300China

[ Objective]This study aimed to compare differences in the pathogenicity between genotype vi Newcastle disease virus （NDV） strains from pigeons and chickens in pigeons. [ Method] Two-month-old pigeons were artificially inoculated with ZJ3 strain from chickens and WX-10-07-Pi strain from pigeons. After inoc- ulation, the clinical symptoms, pathological anatomical changes, tracheal and cloacal detoxification, and histological lesions of experimental pigeons were observed. [ Result] Both ZJ3 strain and WX-10-07-Pi strain could infect pigeons with the incidence rate of 100%, but the mortality rate was 0. The cloacal detoxification time of pigeons in WX-10-07-Pi infection group was longer, and the virus detection rate was higher; in addition, the virus could be detected in various tissues and organs of inoculated pigeons. [ Conclusion] Different genotypes of NDV are pathogenic to pigeons, but the pathogenicity is related to the features of NDV strains. genotype vib NDV from pigeons can be carried and discharged for a long term in pigeons, which can spread in pigeon groups more easily.

关键词： Newcastle disease virus genotype vi Pathogenicity Pigeon Chicken

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