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Regulatory effects of CDT1、RAN genes in human hepatocytesthat were genomic instability induced by radiation

Regulatory effects of CDT1、RAN genes in human hepatocytesth...

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中国生物化学与分子生物学会第十一次会员代表大会暨2014年全国学术会议

作者： Xuhong Dang Yahui Zuo Yayi Yuan Jiangong Liu Hongyan Liu China Institute for radiation Protection

It has been known that genome instability exists in many cancers, and it may be a potentialcause of tumorigenesis. Our studies demonstrated that genomic instability can be inducedby ionizing radiation in human hepatocytes, and CDT1、RAN、HAVCR2 genes were differentialexpressed in human hepatocytes that were genomic instability induced by ionizingradiation. To further investigate the regulatory effects of CDT1、RAN、HAVCR2 genes ongenomic instability induced by ionizing radiation, rna interference was used to silence thegenes including CDT1、RAN respectively in human hepatocytes that were genomic ***, CDT1 and RAN were overexpressed respectively. The apoptosis wasdetected by Annexin Ⅴ-APC flow cytometry(FCM) and the cell cycle was determined byFCM with single PI staining. And the expression changes of CDT1、RAN、HAVCR2、p53genes were assayed by real time fluorescence quantitative PCR technology. The resultsshowed that CDT1 silencing downregulated RAN expression, upregulated HAVCR2、p53genes expression, and inhibited apoptosis;however, overexpression of CDT1 downregulatedRAN、p53 genes and promotes apoptosis. RAN silencing upregulated HAVCR2、p53genes expression and inhibited apoptosis, arrest the cells at G1 phase;however, over expression of RAN down regulated CDT1、 p53 genes and promotes apoptosis. In conclusion,CDT1、RAN、HAVCR2、p53 genes interacted each other, involving genomic instabilitythrough apoptosis regulation and cell cycle regulation.

关键词： genomic instability human hepatocytes rna interference ionizing radiation overexpression

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