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Molecular characterization and genetic analysis of Gnrh2 and Gthβin different ploidy level fishes

Progress in Natural Science:Materials International 2009年第11期19卷 1569-1579页

作者： Yu Long~1,Huan Zhong~1,Shaojun Liu~*,Min Tao,Lin Chen,Jun Xiao,Yun Liu Key Laboratory of Protein Chemistry and Developmental Biology of Education of Ministry of China,College of Life Sciences, Hunan Normal University,Changsha 410081,China Key Laboratory of Protein Chemistry and Developmental Biology of Education of Ministry of China College of Life Sciences Hunan Normal University Changsha 410081 China

The gonadotropin-releasing hormone(GnRH) and the gonadotropin hormone(GTH) play crucial roles in regulating the gonadal development of the *** this study,the Gnrh2,Fshβand LhβcDNAs were cloned and characterized in red crucian carp,triploids and tetraploids,and their phylogenetic relations were comparatively *** the Gnrh2 cDNAs in different ploidy fishes encoded proteins of 86 amino acids,which consisted of a signal peptide,a GnRH2 decapeptide and a GnRH-associated peptide (GAP) linked by a proteolytic cleavage site(Gly-Lys-Arg).The GnRH2 decapeptide and proteolytic cleavage site were absolutely consistent among the three ploidy fishes,but the differences in signal peptide and GAP between diploids and tetraploids were fewer than those between diploids and *** was presumed that the red crucian carp was the original maternal parent of tetraploids,so they had closer *** addition,the Fshβand LhβcDNAs of these three fishes encoded proteins of 130 and 140 amino acids,respectively. Compared with the molecules of other teleosts,the cysteine residues and potential glycosylation sites of Lhβin these three fishes are fully ***,most teleosts of Fshβhad 12 cysteine residues,while those of these three fishes were 13,and 12 of which might form six conserved disulfide bridges by utilizing the cleavage sites between the first and the second cysteine ***,the lack of the second glycosylation site in Fshβof these three fishes might influence the special structure and biological *** the other hand,the phylogenic tree analyses revealed that Gnrh2,Fshβand Lhβhad similar phylogeny relationships among the cyprinids, which indicated that they were conserved in molecular structure and function during the evolution.

关键词： Gnrh2 Gthβ Molecular structure genetic analyses Triploids Tetraploids

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