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Investigating Biological Relationships in Burkina Faso Using DNA Testing

American Journal of Molecular Biology 2025年第1期15卷 97-109页

作者： Bapio Valérie Elvira Jean Télesphore Bazie Moussa Ouattara Serge Théophile Soubeiga Abdou Azaque Zoure Prosper Bado Abel Sorgho Touwendpoulimdé Isabelle Kiendrebeogo Lassina Traore Soumaïla Sawadogo Luc Zongo Théodora M. Zohoncon Florencia W. Djigma Théophane Albert T. Yonli Mahamoudou Sanou Jacques Simpore Centre de Recherche Biomolculaire Pietro Annigoni (CERBA) Ouagadougou Burkina Faso Universit Joseph KI-ZERBO Unit de Formation en Sciences de la Vie et de la Terre Laboratoire de Biologie Molculaire et de Gntique (LABIOGENE) Ouagadougou Burkina Faso Centre National de la Recherche Scientifique et Technologique (CNRST) Institut de Recherche en Sciences de la Sant (IRSS) Ouagadougou Burkina Faso Faculty of Health Sciences University Saint Thomas dAquin (USTA) Ouagadougou Burkina Faso Centre Hospitalier Universitaire Pdiatrie Charles De Gaulle Universit Joseph Ki-Zerbo Ouagadougou Burkina Faso

DNA testing for biological relationships is becoming increasingly common in Burkina Faso. STR analysis remains the most reliable technique for resolving disputes or claims in court regarding biological relationships. This study aimed to establish the links of biological relationships between subjects by analyzing 21 STR loci. The participants were 14 individuals referred to CERBA by the Justice in 2022. Oral or blood samples were taken for each subject. DNA was extracted, and the analysis of DNA polymorphism by PowerPlex® 21 Kit (Part No. DC8902) was performed by capillary electrophoresis on the ABI PRISM 3130 sequencer. DNA profiles were compiled using the GeneMapper IDX software version 1.2. Of the fourteen subjects examined, twelve of these samples had complete genetic profiles, while the other two had partial and absent profiles. The results confirmed the inclusion of three cases of Paternity, one case of maternity, one case of a relationship of brotherhood, and the exclusion of one case of maternity and one case of a relationship of brotherhood. DNA tests improve the resolution of filiations, but they require ethical and cultural awareness and a strengthened legal framework to prevent and protect society.

关键词： DNA STR Identification genetic fingerprint Burkina Faso

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Development of two microsatellite multiplex PCR systems for high throughput genotyping in Populus euphratica

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Journal of Forestry Research 2009年第A3期20卷 195-198页

作者： Eusemann Pascal Fehrenz Steffen Schnittler Martin Department of Botany and Landscape Ecology EMA U Greifswald Grimmer Str. 88 17487 Greifswald Germany

Eighteen microsatellite primer pairs previously developed at Oak Ridge National Laboratory for Populus tremuloides Michx. and Populus trichocarpa Tort. ＆ Gray were screened for amplification in Euphrates poplar, Populus euphratica Oliv. Thirteen loci were found to express polymorphisms ranging from two to 17 alleles. The eight most variable loci were selected to set up and optimize two multiplex polymerase chain reaction （PCR） assays. Three populations containing altogether 436 trees were used to characterize the selected loci and ascertain their applicability for parentage analysis and genotyping studies. Through cross-checking of clonal identity against sex of the genotyped trees we estimated the maximum error rate for merging genotypes to be less than 0.045.

关键词： clone identification Euphrates poplar genetic fingerprint parentage analysis population structure SSR primers

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