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Effect of copper sources and levels on Hydrogen Peroxide generation of Mitochondria from Broiler Hepatocyte

Effect of copper sources and levels on Hydrogen Peroxide Gen...

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中国畜牧兽医学会家畜内科学分会第七届代表大会暨学术研讨会

作者： CAO Hua-bin~(a,b),SU Rong-sheng~b,GUO Jian-ying~b,PAN Jia-qiang~b,LI Ying~b,TANG Zhao-xin~(b*) (aCollege of Animal Science and Technology,JiangXi Agriculture University,middle Lushan road, Nanchang Economic and Technological Development District,Nanchang 330045,P.R.China b Laboratory of Clinical veterinary medicine,College of veterinary,South China Agriculture University, Wushan Avenue,Tianhe District,Guangzhou 510642,P.R.China)

In this study,experiment was performed with the objective of examining the effects of copper sources and levels on hydrogen peroxide(H2O2) generation of mitochondria from broiler *** were sources of Cu(CuS04 versus Cu-Met) fed at four dietary Cu levels.288 broilers(Cobb 500,Gallus domesticus) aged 1 day were randomly divided into 8 groups with 36 each and fed with diets as follows:Controls(Cu 11 mg·kg).and high copper(Cu 110 mg·kg,groupⅡ;Cu 220 mg·kg,high copper groupⅢ;Cu 330 mg·kg,high copper groupⅣ) for 60 days under normal condition, three sample-collections were processed at 12 day-old,36 day-old and 60 day-old respectively to investigate the changes of H202 generation of mitochondria from broiler *** result compared with those of control group,H202 generation of mitochondria in high copper group(Cu 110 mg·kg;Cu 220 mg·kg and Cu 330 mg·kg) were increased of two copper sources(Pgeneration of mitochondria from broiler hepatocyte in each group of Cu-Met were increased compared to those of each group of CuS04(Pgeneration of mitochondria from broiler fed with high dietary copper were mainly concentrated in mitochondrial complex *** results obtained above indicated that high dietary copper could induce oxidized stress damage in liver:at the same level of dietary copper supplement,the organic copper will lead to much faster H2O2 generation velocity of mitochondria from broiler hepatocyte than inorganic *** results can also suggest that mitochondrial complex IV maybe was one of attack target spots under high dietary copper stimulation of organism.

关键词： Broiler Copper Liver Mitochondria H202 generation velocity

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