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Microcalorimetric Studies on gene promoter Function of Cloned DNA Fragements from Halobacterium halobium J7 Plasmid pHH205 in Escherichia coli TG1

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Chinese Journal of Chemistry 2007年第8期25卷 1099-1104页

作者：雷克林侯汉娜刘义叶学成沈萍 College of Chemistry and Molecular Sciences Wuhan University Wuhan Hubei 430072 China Department of Chemistry Xiangfan University Xiangfan Hubei 441003 China State Key Laboratory of Virology Wuhan University Wuhan Hubei 430072 China

Halobacterium halobium is a typical kind of extremely halophilic bacterium. Combined with the antibiotic resistance assay, the microcalorimetric method was used to study the promoter function of the cloned DNA fragments from Halobacterium halobium J7 plasmid pHH205 in Escherichia coli TG1. The promoter probe vector, plasmid pKK232-8, was used to form the recombinants. The DNA fragment, which is the promoter for the chloramphenicol acetyl transferase （CAT） gene in plasmid pKK232-8, is about 800 bp, and the chloramphenicol resistance level presented by IC50 is about 200 μg·mL^-1, which suggests a high promoter activity. The conclusions show that there probably exist double-function or trinary-function gene promoters in Halobacterium halobium, and Archaea may contain rich genetic resources.

关键词： Halobacterium halobium J7 plasmid pKK232-8 gene promoter microcalorimetry

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Myostatin gene promoter:structure,conservation and importance as a target for muscle modulation

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Journal of Animal Science and Biotechnology 2019年第2期10卷 255-273页

作者： Carla Vermeulen Carvalho Grade Carolina Stefano Mantovani Lúcia Elvira Alvares Universidade Federal da Integracao Latino-Americana UNILAInstituto Latino-Americano de Ciências da Vida e da NaturezaAvenida Tarquínio Joslin dos Santos1000Foz do IguacuPR CEP 85870-901Brazil Departamento de Bioquímica e Biologia Tecidual Universidade Estadual de Campinas-UNICAMPRua Monteiro Lobato255CampinasSP CEP 13083-862Brazil.

Myostatin(MSTN) is one of the key factors regulating myogenesis. Because of its role as a negative regulator of muscle mass deposition, much interest has been given to its protein and, in recent years, several studies have analysed MSTN gene regulation. This review discusses the MSTN gene promoter, focusing on its structure in several animal species, both vertebrate and invertebrate. We report the important binding sites considering their degree of phylogenetic conservation and roles they play in the promoter activity. Finally, we discuss recent studies focusing on MSTN gene regulation via promoter manipulation and the potential applications they have both in medicine and agriculture.

关键词： CAAT box E-box gene promoter Myogenesis Myostatin SNP Transcription factors TATA box

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Analysis of the methylation pattern of six gene promoters in sperm of men with abnormal protamination

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Asian Journal of Andrology 2011年第2期13卷 342-346页

作者： Laszlo Nanassy Douglas T Carrell Andrology and IVF Laboratories Department of Surgery University of Utah School of Medicine Salt Lake City UT 84108 USA Department of Obstetrics and Gynecology University of Utah School of Medicine Salt Lake City UT 84208 USA Department of Physiology University of Utah School of Medicine Salt Lake City UT 84108 USA

It has recently been shown that alteration of the methylation pattern of imprinted genes is associated with different types of male infertility. The objective of our study was to investigate the methylation pattern of selected gene promoters in sperm of patients with abnormal protamine replacement. The promoters of OCT4, SOX2, NANOG, HOXC11, miR-17and CREMwere analyzed using bisulfite sequencing and the percentage of DNA methylation was compared between patients with an abnormal protamine l/protamine 2 （P1/ P2） ratio and normozoospermic controls. No significant quantitative differences were found between groups of patients with either an abnormally high or low P1/P2 ratio compared to normal controls. However, two individual samples from infertile subjects （2/20, 10%） showed an altered methylation pattern for the CREMgene promoter that was not found in control samples. These two samples had a significantly higher （P〈0.05） promoter methylation （5.58 and 4.23%, respectively） compared to the control group （0.46%）. In conclusion, in our pilot study, extreme methylations defects were not seen broadly in severely infertile men. However, two patients exhibited altered methylation of the CREMgene, which may be either causative or a result of abnormal protmaine replacement.

关键词： Epigenetic gene promoter male infertility methylation protamine

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Recent advances in molecular biology of gastric carcinogenesis

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Chinese Medical Journal 2003年第1期116卷 3-4页

作者：萧树东冉志华 Shanghai Institute of Digestive Disease

Gastric cancer is a major health care problem and the second most common fatal cancer worldwide. In the last decade, better insight has been gained into the molecular basis underlying the neoplasitc transformation of stomach. The dramatic variation in the incidence of gastric cancer in different geographical areas and from one generation to the next have led to the hypothesis that the incidence of gastric cancer is determined largely by environmental rather than genetic factors.

关键词： HELICOBACTER-PYLORI INFECTION TELOMERASE ACTIVITY MICROSATELLITE INSTABILITY promoter HYPERMETHYLATION MONGOLIAN GERBILS gene promoter CARCINOMA CANCER RISK LESIONS

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