检索结果-南通市图书馆

Comprehensive Analysis of Differential gene expression profile via RNA Sequencing in the Human Ovarian Cancer SKOV3 Cells Treated with Simvastatin

引用

Chemical Research in Chinese Universities 2022年第2期38卷 632-638页

作者： LIU Qianyu DU Zhenwu ZHU He ZHAO Tianyu PAN Ying Department of Gynecology and Obstetrics the China-Japan Union Hospital of Jilin UniversityChangchun 130033P.R.China Department of Orthopedics the Second Hospital of Jilin UniversityChangchun 130041P.R.China Research Centre the Second Hospital of Jilin UniversityChangchun 130041P.R.China Department of Gynecology and Obstetrics the Second Hospital of Jilin UniversityChangchun 130041P.R.China

The experimental studies have demonstrated that some statins have an anticancer effect against ovarian cancer in vitro and in vivo, however, the potential molecular mechanism is still unclear. In the present study, high throughput RNA sequencing(RNA-seq) technology was applied to exploring the mRNA changes treated with Simvastatin in the human ovarian cancer SKOV3 cell line. The result of CCK-8 assay shows that Simvastatin inhibits significantly SKOV3 cell viability in a concentration-dependent manner, and cell cycle according to the flow cytometry analysis result shows cell cycle arrest of G1 phase is induced in SKOV3 cells with 10 μg/mL simvastatin added for 24 h. The differential expression genes(DEGs) analysis of RNA-seq data shows that there are a total of 372 DEGs found in the Simvastatin group, of which 150 mRNAs are up-regulated and 222 mRNAs are down-regulated(fold change>2.0, q value expression of KLF2, RHOB and CIDEB is up-regulated while that of DKK1, AMOTL2 and ANKRD1 is down-regulated. The DEGs are enriched in thirteen significant KEGG pathways including cell cycle, DNA replication, apoptosis, etc. This study provides supported useful information about transcriptome profiles of SKOV3 cell treated with simvastatin, and offers an application basis for the further researches on the mechanism of Simvastatin in the treatment of ovarian cancer.

关键词： Simvastatin Ovarian cancer RNA sequencing gene expression profile

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Analysis of hippocampal gene expression profile of Alzheimer's disease model rats using genome chip bioinformatics

引用

Neural Regeneration Research 2012年第5期7卷 332-340页

作者： Yinghong Li Zhengzhi Wu Yu Jin Anmin Wu Meiqun Cao Kehuan Sun Xiuqin Jia Manyin Chen First Affiliated Hospital of Shenzhen University/Second People＇s Hospital of Shenzhen City Shenzhen 518035 Guangdong Province China Second Clinical Medical College of Jinan University/Shenzhen Institute of Geriatrics Shenzhen 518020 Guangdong Province China

In this study, an Alzheimer＇s disease model was established in rats through stereotactic injection of condensed amyloid beta 1-40 into the bilateral hippocampus, and the changes of gene expression profile in the hippocampus of rat models and sham-operated rats were compared by genome expression profiling analysis. Results showed that the expression of 50 genes was significantly up-regulated （fold change 〉 2）, while 21 genes were significantly down-regulated in the hippocampus of Alzheimer＇s disease model rats （fold change 〈 0.5） compared with the sham-operation group. The differentially expressed genes are involved in many functions, such as brain nerve system development, neuronal differentiation and functional regulation, cellular growth, differentiation and apoptosis, synaptogenesis and plasticity, inflammatory and immune responses, ion channels/transporters, signal transduction, cell material/energy metabolism. Our findings indicate that several genes were abnormally expressed in the metabolic and signal transduction pathways in the hippocampus of amyloid beta 1 40-induced rat model of Alzheimer＇s disease, thereby affecting the hippocampal and brain functions.

关键词： amyloid beta 1-40 Alzheimer＇s disease hippocampus genome chip gene expression profile neural regeneration

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Effects of emodin on gene expression profile in small cell lung cancer NCI-H446 cells

引用

Chinese Medical Journal 2007年第19期120卷 1710-1715页

作者： FU Zhong-yan HAN Jin-xiang HUANG Hai-yan Key Laboratory of Ministry of Health for Biotech-Drug Shandong Medicinal Biotechnology Center Shandong Academy of Medical Sciences Jinan 250062 China Medical School Shandong University Jinan 250012 China

Background The treatment of patients with small cell lung cancer （SCLC） is based on chemotherapy. However, the treatment is limited by the development of drug resistance. Emodin has been shown to exhibit an anti-cancer effect. But the molecular mechanism remains unclear. This study was conducted to investigate the effect of emodin on the gene expression profile changes in SCLC NCI-H446 cells. Methods NCI-H446 cells were treated with emodin and cell viability was determined by MTT assay. Cell apoptosis was determined by both flow cytometry and caspase-3 activity assay. The effect of emodin on the gene expression profile of NCI-H446 cells was analyzed using cDNA microarray. Semi-quantitative reverse transcriptase-polymerase chain reaction （RT-PCR） was used to validate the microarray results. Results Emodin suppressed viability, induced apoptosis and changed cell cycle of NCI-H446 cells. Among the 1262 genes, 10 genes were up-regulated and 8 genes were down-regulated more than 2 folds in NCI-H446 cells when compared with the control cells after treatment with emodin for 12 hours, while 12 genes were up-regulated and 24 genes were down-regulated after treatment with emodin for 24 hours. These genes were involved in metabolism, signal transduction, transcription regulation, cytoskeleton organization, immune response, transport, protein synthesis, cell cycle control, cell adhesion and RNA processing. The RT-PCR results were consistent with those obtained by the microarray. Conclusions Emodin affects the expression of genes involved in various cellular functions and plays important roles in cell apoptosis, tumor metastasis and chemotherapy-resistance, which suggests emodin might become an effective chemopreventive or chemotherapeutic agent for SCLC.

关键词： emodin small cell lung cancer gene expression profile

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Effects of Panaxadiol Saponins Component as A New Chinese Patent Medicine on Proliferation,Differentiation and Corresponding gene expression profile of Megakaryocytes

引用

Chinese Journal of Integrative Medicine 2016年第1期22卷 28-35页

作者：温伟为孙馨庄海峰林筱洁郑智茵高瑞兰尹利明 The Third People's Hospital of Hangzhou Hangzhou 310009China Department of Oncology Zhejiang Provincial People's HospitalHangzhou 310014China Institution of Hematology Research the First Affiliated Hospital of Zhejiang Chinese Medical UniversityHangzhou 310006China

Objective： To investigate the effects of panaxadiol saponins component （PDS-C） isolated from total saponins of panax ginseng on proliferation, differentiation and corresponding gone expression profile of megakaryocytes. Methods： Bone marrow culture of colony forming assay of megakaryocytic progenitor cells （CFU-MK） was observed for the promoting proliferation mediated by PDS-C, and differentiation of megakaryocytic blasts caused by PDS-C was analyzed with flow cytometry in CHRF-288 and Meg-01 cells, as well as proliferation, differentiation-related genes expression profile and protein expression levels were detected by human gone expression microarray and western blot. Results： In response to PDS-C 10, 20 and 50 mg/L, CFU-MK from 10 human bone marrow samples was increased by 28.9± 2.7%, 41.0% ± 3.2% and 40.5% ± 2.6% over untreated control, respectively （P〈0.01, each）. Flow cytometry analysis showed that PDS-C treated CHRF-288 cells and Meg-01 cells significantly increased in CD42b, CD41, TSP and CD36 positive ratio, respectively. PDS-C induced 29 genes up-regulated more than two-fold commonly in both cells detected by human expression microarray representing 4000 known genes. The protein expression levels of ZNF91, c-Fos, BTF3a, GATA-1, RGS2, NDRG2 and RUNX1 were increased with western blot in correspond to microarray results. Conclusion： PDS-C as an effective component for hematopoiesis, play the role to enhance proliferation and differentiation of megakaryocytes, also up-regulated expression of proliferation, differentiation-related genes and proteins in vitro. KEYWORDS panaxadiol saponins, megakaryocyte, gone expression profile, proliferation, differentiation

关键词： panaxadiol saponins megakaryocyte gene expression profile proliferation differentiation

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Analysis of gene expression profile in Lung Adenosquamous Carcinoma Using cDNA Microarray

引用

Wuhan University Journal of Natural Sciences 2004年第6期9卷 967-972页

作者： YANGFei YANGJiong JIANGMan YEBo ZHANGYu-xia CHENHong-lei XIADong LIUMing-qiu DepartmentofPathology SchoolofMedicineWuhanUniversityWuhan430071HubeiChina DepartmentofRespiratoryMedicine RenminHospitalWuhanUniversityWuhan430060HubeiChina

gene expression profile of the lung adenosquamous carcinoma was characterized by using cDNA microarray chip containing 4 096 human genes. Among target genes, 508 differentially expressed genes were identified in adenosquamous carcinoma of the lung, 232 genes were overexpressed and 276 genes were underexpressed. Among them, 92 genes are cell signals transduction genes, 34 genes are proto-oncogenes and tumor suppressor genes or cell cycle related genes or cell apoptosis related genes, 29 genes are cell skeleton genes, 28 genes are DNA synthesis, repair and recombination genes, 12 genes are DNA binding and transcription genes. These genes may be associated with the occurence and development of adenosquamous carinome of the lung. Key words lung carcinoma - adenosquamous carcinoma - microarray - gene expression profile CLC number R 734.2 Foundation item: Supported by the National Natural Science Foundation of China (39870305)Biography: YANG Fei(1972-), female, Ph. D candidate, research direction: etiology and pathogenesis of lung cancer.

关键词： lung carcinoma adenosquamous carcinoma microarray gene expression profile

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

THE gene expression profile OF HIGHLY METASTATIC HUMAN OVARIAN CANCER CELL LINE BY gene CHIP

引用

Chinese Journal of Cancer Research 2001年第4期13卷 265-270页

作者：吕桂泉许沈华牟瀚舟朱赤红羊正炎高永良楼洪坤刘祥麟杨文程勇 Zhejiang Cancer Research Institute Hangzhou 310022 Department of Gynecology Zhejiang Cancer Hospital Hangzhou United gene Scientific Tech. (Group) Col. Ltd. Shanghai 200092

Objective: To study the gene expression of high metastatic human ovarian carcinoma cell line (HO-8910PM) and to screen for novel metastasis- associated genes by cDNA microarray. Methods: The cDNA was retro-transcribed from equal quantity mRNA derived from tissues of highly metastatic ovarian carcinoma cell line and normal ovarian, and was labeled with Cy5 and Cy3 fluorescence as probes. The mixed probes were hybridized with BioDoor 4096 double dot human whole gene chip. The chip was scanned by scanArray 3000 laser scanner. The acquired image was analyzed by Imagene 3.0 software. Results: By applying the cDNA microarray we found: A total of 323 genes whose expression level were 3 times higher or lower in HO-8910PM cell than normal ovarian epithelium cell were screened out, with 71 higher and 252 lower respectively. Among these 10 were new genes. 67 genes showed expression difference bigger than 6 times between HO-8910PM cell and normal ovarian epithelium cell, among these genes 12 were higher, 55 lower, and two new genes were found. Conclusion: cDNA microarray technique is effective in screening the differentially expressed genes between human ovarian cancer cell line (HO-8910PM) and normal ovarian epithelium cell. Using the cDNA microarray to analyze of human ovarian cancer cell line gene expression profile difference will help the gene diagnosis, treatment and protection.

关键词： Ovarian cancer High metastasis cDNA microarray gene expression profile

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Differentially gene expression profile Related to Inflammation in Endometrial Cells Induce by Lipopolysaccharide

引用

Journal of Reproduction and Contraception 2009年第1期20卷 27-34页

作者： Li-hua QIN Ruo-guang WANG Sheng LI Chun-mei LI Guangzhou University of TCM College of the Second Clinical Medicine Guangzhou 510405 China Institute of Combined Traditional Chinese and Western Medicine Hunan University of TCM Changsha 410007.China

Objective To investigate differentially expressed genes related to inflammation in endometrial cells induced by Lipopolysaccharide（LPS）. Methods Normal endometrium in the proliferative phase of specimen from 3 cases for the experiment was collected. The LPS group were treated with 50 μg/ml LPS. Total RNA was extracted using Trizol reagent from cells. RNA quality was assessed by determining the OD260/280 ratio by agarose gel electrophoresis, the chip was scanned by laser scanner. The acquired was analyzed. Results A total of differentially expressed genes were found, these genes were relative to many aspects. Among them, the expression of genes involved in inflammation were up-regulated by LPS, such as overexpression of IL-1β, 8, etc. Conclusion The results indicates that inflammation-related genes may be one of the mechanisms of abnormal uterine bleeding by LPS-induced.

关键词： gene expression profile lipopolysaccharide（LPS） endometrial cell inflammation

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Identifying genetic susceptibility to Aspergillus fumigatus infection using collaborative cross mice and RNA-Seq approach

引用

Animal Models and Experimental Medicine 2024年第1期7卷 36-47页

作者： Roa'a H.S.Yosief Iqbal M.Lone Aharon Nachshon Heinz Himmelbauer Irit Gat-Viks Fuad A.Iraqi Department of Clinical Microbiology and Immunology Sackler Faculty of MedicineTel-Aviv UniversityTel-AvivIsrael School of Molecular Cell Biology and Biotechnology George S.Wise Faculty of Life SciencesTel Aviv UniversityTel AvivIsrael Institute of Computational Biology Department of BiotechnologyUniversity of Natural Resources and Life SciencesMuthgasse 181190 ViennaAustria

Background:Aspergillus fumigatus(Af)is one of the most ubiquitous fungi and its infection potency is suggested to be strongly controlled by the host genetic back-ground.The aim of this study was to search for candidate genes associated with host susceptibility to Aspergillus fumigatus(Af)using an RNAseq approach in CC lines and hepatic gene expression.Methods:We studied 31 male mice from 25 CC lines at 8 weeks old;the mice were infected with Af.Liver tissues were extracted from these mice 5 days post-infection,and next-generation RNA-sequencing(RNAseq)was performed.The gene-E analysis platform was used to generate a clustered heat map matrix.Results:Significant variation in body weight changes between CC lines was ob-served.Hepatic gene expression revealed 12 top prioritized candidate genes differ-entially expressed in resistant versus susceptible mice based on body weight changes.Interestingly,three candidate genes are located within genomic intervals of the previ-ously mapped quantitative trait loci(QTL),including Gm16270 and Stox1 on chromo-some 10 and Gm11033 on chromosome 8.Conclusions:Our findings emphasize the CC mouse model's power in fine mapping the genetic components underlying susceptibility towards Af.As a next step,eQTL analysis will be performed for our RNA-Seq data.Suggested candidate genes from our study will be further assessed with a human cohort with aspergillosis.

关键词： aspergillus fumigatus infection collaborative cross(CC)mice gene expression profile gene-network host susceptibility quantitative trait loci(QTL)mapping RNA-Seq

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Study on the Effect of Youguiyin(右归饮) on Regulating gene expression profile of the Rats with Femoral Head Necrosis Induced by Steroid

引用

Chinese Journal of Integrative Medicine 2012年第4期18卷 269-275页

作者：吴承亮毛强金红婷肖鲁伟童培建 Zhejiang Traditional Chinese Medicine University Zhejiang Provincial Institute of Ortopaedics and Traumatology Ortopaedics and Traumatology Department of Zhejiang Provincial Traditional Chiese Medicine Hospital the First Affiliated Hospital of Zhejiang Traditional Chinese Medicine University

Objective： To observe the regulation of Youguiyin （YGY, 右归钦） on the gene expression profile of the rat with steroid-induced femoral head necrosis （sFHN）, for the sake of investigating its molecular mechanism of sFHN prevention and treatment. Methods： All the 30 rats were randomly divided into three groups, the normal control group （A）, the model control group （B）, and the YGY treated group （C）, 10 in each group. After rats in Groups B and C were being made into FHN models with steroid injection, they received a daily intragastdc administration of saline and YGY respectively in equal volume for a total of 6 weeks, while to the unmodeled normal rats in Group A, saline was administered instead. The rats were sacrificed at the terminal of administration; their mRNA from femoral head tissue was extracted and prepared to cDNA probe through inverse transcription for detecting gene expression profile by microarray, outcomes of which was passing fluorescence quantitative PCR verification, and the differential expressed genes were analyzed adopting gene ontology （GO） method. Results： Compared with Group A, the numbers of differential genes found in Groups B and C were 190 and 92, respectively, but the changing trend in the two groups was opposite, mainly manifested as down-regulating in Group B/Group A （GB/GA） and up-regulating in Group C/Group B （GC/GB）. The analysis showed that these differential genes were mainly assigned to cell apoptosis, signal transduction, metabolism, cell proliferation and differentiation, cell cycle, blood coagulation, antioxidant activity, etc. Conclusions： sFHN was regulated by various genes; the regulation of YGY on expressions of these genes and the intra]extra-cellular signaling processes was possibly the molecular mechanism of YGY for preventing/treating sFHN. This study gave an explanation to the effectiveness of Chinese medicine in preventing/treating FHN from aspects of gene expression and enriched the Chinese medicine theory of ＂Kidney （Shen） governing bones＂.

关键词： Youguiyin steroid-induced femoral head necrosis gene chip gene expression profile molecular mechanism

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

gene expression profile of Developing Cassava Storage Roots Revealed an Active Process of Glycolysis/Gluconeogenesis

Gene Expression Profile of Developing Cassava Storage Roots ...

引用

中国植物生理学会第十次会员代表大会暨全国学术年会

作者： Jun Yang, Dong An, Peng Zhang* SIBS-ETH Shanghai Center for Cassava Biotechnology, National Laboratory of Plant Molecular genetics, Institute of Plant Physiology & Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai 200032, China

＜正＞Cassava storage root is a major sink for photosynthetic carbon assimilation. Its formation involves changes of transcriptional regulation and metabolic activities. For example, to accumulate starch in its parenc... 详细信息

关键词： cassava storage root development gene expression profile microarray

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：