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Analysis of gene effect on Four Characters of Immature Embryo Culture in Maize

Agricultural Sciences in China 2007年第11期6卷 1291-1296页

作者： SONG Yun XIA Yan-li WEI Xin ZHANG Zhi-ming ZHAO Mao-jun RONG Ting-zhao PAN Guang-tang Maize Research Institute Sichuan Agricultural University Yaan 625014 P.R.China Key Laboratory of Crop genetic Resources and Improvement Ministry of Education/Sichuan Agricultural University Yaan 625014 P.R. China College of Life Science Shanxi Agricultural University Taigu 030801 P.R.China Sichuan Institute of Chinese Material Medica Chengdu 610041 P.R.China

This study was to find the regularity in the hereditary variation for the main culturing characters of the immature embryo culture in maize. Two kinds of inbred-line, R18-599 （red） with very excellent embryo culturing capacity and R15 with very poor embryo culturing capacity, were used as P1 and P2 for obtaining six generations. By culturing immature embryos of the six generations, four culturing characters, namely embryonic callus induction efficiency, nonembryonic callus induction efficiency, cloning ability of the embryonic callus, and number of regenerating plants, were analyzed using the general mean analysis and generation joint analysis. Results showed that the embryonic callus induction efficiency accorded with two major additive-dominance-epistatic genes and polygene-mixed additive-dominance-epistatic inheritance model. The induction efficiency of the nonembryonic callus accorded with two major additive-dominance-epistatic genes. The number of regenerating plants accorded with one major gene and polygene-mixed additive-dominance inheritance model. The cloning ability of the embryo callus accorded with two major genes and polygene-mixed inheritance model, whereas the effect of epistatic gene on this character was identified results of the two methods, generation joint analysis may genetic information. to be different using the two methods. By comparison of the not only raise experimental precision but also provide more

关键词： maize (Zea mays L.) immature embryo culture inheritance model gene effect

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Isolation, Identification and Earliness effect Analysis of Rice Dominant Earliness gene Ef-cd

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Agricultural Sciences in China 2005年第8期4卷 561-566页

作者： DENG Xiao-jian WANG Ping-rong LI Xiu-lan DONG Chun-lin YANG Zhi-rong Rice Research Institute Sichuan Agricultural University Chengdu 611130 P.R.China College of Life Sciences Sichuan University Chengdu 610064 P.R.China

Ef-cd gene is a dominant earliness gene located on the short arm of rice chromosome 3. In this paper, through continuous backcross, self-pollination and molecular marker assisted selection, individual Ef-cd gene was isolated and its nearly isogenic lines were constructed by using early-maturing indica line 6442S-7 as the donor parent, and by using latematuring indica line Minghui 63 （MH63）, Shuhui 881 （SH881） and Shuhui 527 （SH527） as the recurrent parents （genetic background）, respectively. Further, it was found out that Ef-cd gene could generally advance rice to head 11-14 d earlier. So, it was considered that Ef-cd gene played an important role in rapid developing early-maturing and super high-yielding rice varieties.

关键词： Rice Growth duration Earliness Dominant earliness gene gene effect

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effect of Rolled Leaf gene Rl_(t) on Grain Quality in Hybrid Rice

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Rice science 2005年第3期12卷 168-172页

作者： SHAO Yuan-jian CHEN Zong-xiang CHEN Hong-qi ZHANG Ya-fang PAN Xue-biao Agronomy College Yangzhou University Yangzhou 225009 China Nantong Agricultural Vocational College Nantong 226007China China National Rice Research Institute Hangzhou 310006 China

effects of rolled leaf gene Rl（t） on grain quality characters of hybrid rice were analyzed by using three pairs of rolled leaf near-isogenic lines under two fertilizer treatments. Under normal fertilizer level （e.g. 450 kg urea per ha）, head rice rates and milled rice recovery of rolled leaf hybrids were significantly higher than those of corresponding non-rolled crosses, while the chalky rice rate and chalkiness were all lower. Of the RVA profiles, the peak viscosity, the hot paste viscosity and the breakdown viscosity of the rolled were all higher than those of the corresponding non-rolled ones to various degrees. Increasing fertilizer application for promoting panicle development increased the brown, milled and head rice rates except for Shanyou 63, furthermore, significant difference of head rice rates existed between the rolled leaf Shanyou 559 and Shanyou 559; while the peak viscosity, the hot paste viscosity and the breakdown viscosity all decreased to different levels; changes of values of other characters had no apparent regularity. It suggested that Rl（t） could improve rice quality under certain conditions.

关键词： hybrid rice rice quality rolled leaf gene effect starch viscosity rapid visco analyzer

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Studies on the Heredity of the Traits Related to the Photoperiod-Sensitive Phenomenon Among the Temperate ×Tropical Crosses in Maize

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Agricultural Sciences in China 2003年第2期2卷 119-126页

作者： CHEN Yan-hui, ZHANG Xiang-qian, CHANG Sheng-he, WU Lian-cheng,WU Jian-yu and XI Zhang-yingAgronomy College , Henan Agricultural University , Zhengzhou 450002 , P. R. China Agronomy College Henan Agricultural University Zhengzhou 450002 P. R. China

The genetic study of characters concerning photoperiod sensitivity of the growth and development stages, plant and ear height was carried out from 5 temperate tropical combinations by using P1 , P2, F1, F2, BC1, BC2 generations mean analysis. The results showed that the square sums of additive, of dominance and of epistatic gene effects contributed to total genetic variance were 11. 3 -53. 6%, 36.0 -78. 6% and 5.5 -27.0% respectively for five traits of the insensitive×insensitive combination to photoperiod, which suggested dominance gene effect was the most important among various gene effects. In insensitive X sensitive combination to photoperiod, the square sums of additive gene effect with 66. 9 - 84. 5 %, of dominance gene effect with 11. 6 30. 7% and of epistatic gene effect with 1.4-5. 2% were found for five tested traits, which showed additive gene effect held a dominant position, and dominance and epistatic gene effects were reduced compared with insensitive×insensitive combination. The differences existed among different insensitive×sensitive combinations. The proportion of the genetic component among the insensitive×sensitive crossbreed offspring in the temperate zone had a certain dosage effect on fading photoperiod sensitivity reaction. The sensitivity of BC1 , with 25 % of the tropical genetic component, is extremely weak, but with high application value.

关键词： Maize Photoperiod sensitivity characters gene effect generation analysis

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