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Biotechnological preparation of chicken skin gelatine using factorial design of experiments

Food Bioscience 2022年第3期47卷 898-907页

作者： Petr Mrázek Robert Gál Pavel Mokrejš Jana Orsavová Dagmar Janáčová Tomas Bata University in Zlín Department of Polymer EngineeringVavrečkova 275760 01ZlínCzech Republic Tomas Bata University in Zlín Department of Food TechnologyMostní 5139760 01ZlínCzech Republic Tomas Bata University in Zlín Language CentreŠtefánikova 5670760 01ZlínCzech Republic Tomas Bata University in Zlín Department of Automation and Control EngineeringNad Stráněmi 4511760 01ZlínCzech Republic

Poultry meat production steadily increases and its by-products including legs or skins may be further utilized to prepare gelatine,generally by using alkali or acid *** they are toxic,different methods are desirable,*** proteolytic *** enzymes is beneficial as they shorten processing time and reduce the required water *** conditions(the enzyme amount during the pre-treatment,first and second extraction temperature and time)of the process may influence gelatine quality and its *** this study,gelatines were prepared from chicken skin at different conditions in the series of combined factorial design of *** significance of the influence of the conditions on the quality(gel strength,viscosity,melting and gelling point)and yield of chicken skin gelatine(CSG)was *** highest yield(31.5%)was obtained at the conditions of the enzyme amount of 0.8%,first extraction temperature 80℃ and time 30 min,second extraction temperature 90℃ and time 60 ***,the highest CSG quality of gel strength(190 Bloom),viscosity(4.06 mPas),melting point(38.3℃)and gelling point(20.5℃)was obtained at the conditions of 0.2%/50℃/30 min in the first extraction.

关键词： Collagen Chicken skin Enzyme pre-treatment gelatine Gelling properties

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Mechanical property and biocompatibility of co-precipitated nano hydroxyapatite-gelatine composites

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Journal of Advanced Ceramics 2015年第3期4卷 237-243页

作者： Huirong LE Kiruthika NATESAN Sundaram PRANTI-HARAN School of Marine Science and Engineering Plymouth UniversityUnited Kingdom Peninsular Schools of Medicine and Dentistry Plymouth UniversityUnited Kingdom School of Engineering Physics and MathematicsUniversity of DundeeUnited Kingdom

Uniform and well dispersed nano hydroxyapatite(HA)-gelatine composites were obtained by co-precipitation of hydroxyapatite and denatured calf skin *** process allowed much higher concentration of hydroxyapatite to be produced over conventional hydrothermal process to improve the *** effect of gelatine on the morphology,mechanical properties,and biocompatibility of hydroxyapatite particles was *** cell tests of the consolidated hydroxyapatite-gelatine composites showed that the composites have excellent biocompatibility.

关键词： biomaterials nanocomposites hydroxyapatite(HA) gelatine biocompatibility

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Microstructure and Mechanical Properties of Calcium Phosphate Cement/gelatine Composite Scaffold with Oriented Pore Structure for Bone Tissue Engineering

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Journal of Wuhan University of Technology(Materials Science) 2012年第1期27卷 92-95页

作者：漆小鹏叶建东 YE Jiandong School of Materials Science and Engineering South China University of Technology School of Material and Chemical Engineering Jiangxi University of Science and Technology

The macroporous calcium phosphate（CPC） cement with oriented pore structure was prepared by freeze casting. SEM observation showed that the macropores in the porous calcium phosphate cement were interconnected aligned along the ice growth direction. The porosity of the as-prepared porous CPC was measured to be 87.6% by Archimede＇s principle. XRD patterns of specimens showed that poorly crystallized hydroxyapatite was the main phase present in the hydrated porous calcium phosphate cement. To improve the mechanical properties of the CPC scaffold, the 15% gelatine solution was infiltrated into the pores under vacuum and then the samples were freeze dried to form the CPC/gelatine composite scaffolds. After reinforced with gelatine, the compressive strength of CPC/gelatine composite increased to 5.12 MPa, around fifty times greater than that of the unreinforced macroporous CPC scaffold, which was only 0.1 MPa. And the toughness of the scaffold has been greatly improved via the gelatine reinforcement with a much greater fracture strain. SEM examination of the specimens indicated good bonding between the cement and gelatine. Participating the external load by the deformable gelatine, patching the defects of the CPC pores wall, and crack deflection were supposed to be the reinforcement mechanisms. In conclusion, the calcium phosphate cement/gelatine composite with oriented rmre structure nrenared in this work might be a potential scaffold for bone tissue engineerinm

关键词： calcium phosphate cement scaffold gelatine oriented pores mechanical properties

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A Qualitative Morphological Comparison of Two Haemostatic Agents in a Porcine Liver Trauma Model

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Surgical Science 2013年第8期4卷 359-364页

作者： Kenneth E. Coenye Claire Bourgain Claudia Keibl Sylvia Nürnberger Martijn van Griensven Department for Pathological Anatomy Free University Brussels (VUB) Brussels Belgium Departments for Surgery and Emergency Medicine AZ Jan Portaels Vilvoorde Belgium Ludwig Boltzmann Institute for Experimental and Clinical Traumatology in the Research Centre of the AUVA Vienna Austria

Background: Many surgical haemostatic agents are available which aim to reduce morbidity and mortality of bleeding, as well as associated costs. We used qualitative techniques to compare the effectiveness of two topical gelatine-based haemostatic agents in a porcine liver trauma model. Methods: We compared the activity of Floseal? (with human or bovine thrombin), Surgiflo? and Surgiflo? plus 5000 IU bovine thrombin per 10 mL of product (n = 6 test sites/group). Different clinical scenarios were evaluated in two anaesthetised piglets;normal physiological conditions (normotension and normothermia), and a status of hypotension, hypothermia and haemodilution, requiring inotropic support. Laparotomy was performed, and five identical stab wounds were made on each liver lobe. Four of these wounds were randomly treated with one of the agents under investigation, while one wound was kept as an untreated control. Haemostasis was observed during and immediately following surgery. After euthanisation, wounds were examined (blinded for treatment) macroscopically, microscopically and using scanning electron microscopic evaluation. Results: Surgiflo? produced some degree of clotting in 1/3 applications in both piglets. Co-treatment with thrombin resulted in a minor improvement in performance in the animal with normal physiological conditions (some degree of clotting in all wounds);no improvement was seen in the animal in a status of hypotension, hypothermia and haemodilution. Floseal? induced clotting in all wounds for both piglets. Microscopic and scanning electron microscope examination of the stab wounds demonstrated that Floseal? created a stable, dense agglomerate of gelatine and fibrin, which firmly adhered to the adjacent liver tissue. In wounds treated with Surgiflo? or Surgiflo? plus thrombin, the gelatine contained more air bubbles, resulting in less fibrin inclusion in the clot and weaker adhesion of the clot to the liver tissue. Conclusion: Floseal? created a dense and

关键词： Haemostasis Coagulation Thrombin Fibrin gelatine Sealant Clot Surgery Trauma Wound Healing

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