检索结果-南通市图书馆

NKX6.3 protects against gastric mucosal atrophy by downregulatingβ-amyloid production

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2019年第3期25卷 330-345页

作者： Jung Hwan Yoon Yeon Soo Lee Olga Kim Hassan Ashktorab Duane T Smoot Suk Woo Nam Won Sang Park Department of Pathology College of Medicine The Catholic University of Korea Functional RNomics Research Center College of Medicine The Catholic University of Korea Department of Hospital Pathology College of Medicine The Catholic University of Korea Department of Medicine Howard University Department of Medicine Meharry Medical Center

BACKGROUND Atrophic gastritis is characterized by loss of appropriate glands and reduction in gastric secretory function due to chronic inflammatory processes in gastric mucosa. Moreover, atrophic gastritis is considered as a precancerous condition of gastric cancer. However, little is known about the molecular mechanism underlying gastric mucosal atrophy and its contribution to gastric carcinogenesis.Thus, we hypothesized that transcription factor NKX6.3 might be involved in maintaining gastric epithelial homeostasis by regulating amyloid β(Aβ)production.AIM To determine whether NKX6.3 might protect against gastric mucosal atrophy by regulating Aβ production.METHODS We identified NKX6.3 depletion induced cell death by cell count and Western blot assay. Production and mechanism of Aβ oligomer were analyzed by enzymelinked immunosorbent assay, Western blot, immunoprecipitation, real-timequantitative polymerase chain reaction and immunofluorescence analysis. We further validated the correlation between expression of NKX6.3, Helicobacter pylori CagA, Aβ oligomer, apolipoprotein E(ApoE), and β-secretase 1(Bace1) in 55 gastric mucosae.RESULTS NKX6.3 depletion increased both adherent and floating cell populations in HFE-145 cells. Expression levels of cleaved caspase-3,-9, and poly ADP ribose polymerase were elevated in floating HFE-145^(shNKX6.3) cells. NKX6.3 depletion produced Aβ peptide oligomers, and increased expression of ApoE, amyloid precursor protein, Aβ, Bace1, low-density lipoprotein receptor, nicastrin, high mobility group box1, and receptor for advanced glycosylation end product proteins. In immunoprecipitation assay, γ-secretase complex was stably formed only in HFE-145^(shNKX6.3) cells. In gastric mucosae with atrophy, expression of Aβpeptide oligomer, ApoE, and Bace1 was detected and inversely correlated with NKX6.3 expression. Treatment with recombinant Aβ 1-42 produced Aβoligomeric forms and decreased cell viability in HFE-145^(shNKX6.3) cells. Additionally,NKX6.3 depletion increased expression of in

关键词： NKX6.3 Gastric mucosa Atrophy Amyloid β gastrokine 1

gastrokine2在胃癌中的表达及临床意义

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

中华临床医师杂志（电子版） 2013年第20期7卷 18-21页

作者：王峰时林森汪灏王萌冯敏管文贤南京大学医学院附属鼓楼医院普通外科 210008

目的探讨gastrokine 2在胃癌的发生、发展过程中的作用。方法应用实时荧光定量聚合酶链反应(RT-PCR)和Western blot印迹法检测76例诊断明确的胃癌患者的癌组织及癌旁组织中gastrokine2的表达,并分析胃癌组织中mRNA和蛋白的表达与临床病... 详细信息

目的探讨gastrokine 2在胃癌的发生、发展过程中的作用。方法应用实时荧光定量聚合酶链反应(RT-PCR)和Western blot印迹法检测76例诊断明确的胃癌患者的癌组织及癌旁组织中gastrokine2的表达,并分析胃癌组织中mRNA和蛋白的表达与临床病理特征之间的关系。结果 gastrokine 2 mRNA和蛋白在胃癌组织中的表达均低于癌旁组织,差异有统计学意义(Pgastrokine 2 mRNA的表达与肿瘤的位置、浸润深度、分化程度、Lauren分型、淋巴结转移及患者的性别和年龄均无关(P>0.01),而幽门螺杆菌(HP)阳性胃癌患者的癌组织中gastrokine 2 mRNA表达低于HP阴性者(Pgastrokine 2 mRNA表达量高于Ⅱ、Ⅲ、Ⅳ期胃癌患者(Pgastrokine 2蛋白的表达与肿瘤的位置、浸润深度、分化程度、Lauren分型、淋巴结转移、TNM分期、幽门螺杆菌感染及患者的性别和年龄均无关(P>0.01)。结论 gastrokine 2在胃癌组织中的表达显著降低,它作为一种保护性基因在胃癌的发生和发展中扮演着重要角色,随着胃癌的进展,其抑制肿瘤的作用降低,同时HP感染可能减弱其对胃黏膜的保护作用。

关键词：胃肿瘤 gastrokine 2基因螺杆菌,幽门实时荧光定量聚合酶链反应印迹法,蛋白质

gastrokine 1在胃癌组织中的表达及其与胃癌临床病理特征的关系

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

中国普外基础与临床杂志 2012年第10期19卷 1089-1093页

作者：陈佳慧肖江卫任明扬魏寿江田小兵周彤谢贤镛王崇树南充市中心医院川北医学院第二临床学院普外二科四川南充637000 川北医学院附属医院普外一科四川南充637000 川北医学院肝胆胰肠研究所四川南充637000 川北医学院四川南充637000 川北医学院附属医院病理科四川南充637000

目的探讨gastrokine 1在胃癌的发生、发展过程中的作用。方法应用实时荧光定量逆转录聚合酶链反应(RT-PCR)和免疫组织化学方法检测52例诊断明确的胃癌患者的癌组织及癌旁组织中gastrokine 1的表达,并分析其与胃癌患者临床病理学特征的... 详细信息

目的探讨gastrokine 1在胃癌的发生、发展过程中的作用。方法应用实时荧光定量逆转录聚合酶链反应(RT-PCR)和免疫组织化学方法检测52例诊断明确的胃癌患者的癌组织及癌旁组织中gastrokine 1的表达,并分析其与胃癌患者临床病理学特征的关系。结果 gastrokine 1基因和蛋白在胃癌组织中的表达均低于癌旁组织〔基因△Ct值:9.07±5.06比0.85±3.79;蛋白表达阳性率:5.8%(3/52)比86.5%(45/52)〕,差异均有统计学意义(Pgastrokine 1基因的表达与肿瘤的位置、浸润深度、分化程度、淋巴结转移及分期,患者的性别和年龄以及术前外周血CEA及CA19-9水平均无关(P>0.05),而幽门螺旋杆菌(Helicobacter pylori,HP)阳性胃癌患者的癌组织中gastrokine 1基因表达低于HP阴性者(△Ct值分别为11.14±5.19和7.67±4.18,Pgastrokine 1在胃癌组织中的表达显著降低,它作为一种保护性基因在胃癌的发生和发展中扮演着重要角色,它的保护作用可能因HP感染而减弱。

关键词： gastrokine 1基因胃癌幽门螺旋杆菌

Down-Regulation of GKN1 in Gastric Cancer Is Not Associated with the RUNX3 Expression

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Journal of Biosciences and Medicines 2017年第11期5卷 80-90页

作者： Chiara Stella Di Stadio Filomena Altieri Antonella Federico Giuseppina Miselli Antonello Niglio Maurizio De Palma Emilia Rippa Paolo Arcari Department of Molecular Medicine and Medical Biotechnology University of Naples Federico II Naples Italy Hospital A. Cardarelli Naples Italy CEINGE Advanced Biotechnology Scarl Naples Italy

gastrokine 1 (GKN1) is a gastric mucosal protein highly expressed and secreted in normal individuals but during Helicobacter pylori infection or in gastric carcinogenesis it is strongly down-regulated or totally absent. In gastric cancer, the GKN1 gene is silenced through an epigenetic mechanism most likely mediated by a transcription factor that promotes on GKN1 promoter the activity of the enzymes SUV39H1 and HDACs. Because RUNX3 is a potential candidate in the regulation of molecular carcinogenesis process of stomach cancers, we tried to assess if RUNX3 could be involved in GKN1 down-regulation in GC. 17 paired of non-tumoral and tumoral surgical specimens from patients with gastric cancer were analyzed for GKN1 and RUNX3 by Western blotting and chromatin immunoprecipitation (Chip) assays. The overall results indicated that RUNX3 expression was not associated with the down-regulation of GKN1. The expression levels of RUNX3 in non-tumoral and tumoral samples suggest that RUNX3 does not act as a tumor suppressor but that it might play a complex oncogenic role in gastric cancer cells.

关键词： gastrokine 1 Gastric Cancer Epigenetics RUNX3 Tumor Suppressor

Association of H3K4me3 and CBX7 Levels to the Down-Regulation of <i>GKN</i>1 Gene Expression in Gastric Cancer Cells

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Biosciences and Medicines 2019年第5期7卷 146-156页

作者： Chiara Stella Di Stadio Filomena Altieri Antonella Federico Giuseppina Miselli Maurizio Grillo Emilia Rippa Paolo Arcari Department of Molecular Medicine and Medical Biotechnology University of Naples Federico II Naples Italy Department of Surgery Antonio Cardarelli Hospital Naples Italy CEINGE Advanced Biotechnology Scarl Naples Italy

gastrokine 1 (GKN1) is a highly secreted gastric mucosal protein in normal individuals but strongly down-regulated or totally absent in gastric cancer subjects. An epigenetic mechanism might be responsible for GKN1 gene silencing probably through the activity of a transcription factor in association with the enzymes SUV39H1 and HDACs on the GKN1 promoter. In fact, compared to non-tumor tissues, a high increase of H3K9me3 level was observed in the corresponding tumor ones. Because H3K4me3 seems to be a possible epigenetic mark for active euchromatin, we try to verify the H3K4me3 level on the GKN1 promoter in gastric cancer tumor specimens. In addition, we also attempt to highlight if CBX7 could be the possible regulatory transcription factor correlated to GKN1 gene promoter. Therefore, we evaluated if the CBX7 expression levels could be associated with GKN1 down-regulation in gastric cancer. To this purpose, 2 pairs of non-tumor and tumor surgical specimens from patients with gastric cancer were analyzed for H3K4me3 by chromatin immunoprecipitation (ChiP) assays, and 9 pairs were instead analyzed by Western blotting for GKN1, and CBX7 expression levels, respectively. The results suggested that the observed increase of H3K4me3 in tumor samples was not in agreement with its proposed function whereas the expression of CBX7 was not associated with the down-regulation of GKN1. In particular, the expression levels of CBX7 in tumor samples might suggest a survival role in gastric cancer.

关键词： CBX7 gastrokine 1 Gastric Cancer Epigenetics H3K4 Methylation

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

GKN、TFF蛋白与胃癌

胃肠病学 2011年第6期16卷 373-375页

作者：王钰虹陈洁陈旻湖中山大学附属第一医院消化内科 510080

gastrokine(GKN)和三叶因子家族(TFF)是在正常胃黏膜上皮细胞中大量特异性表达且结构和功能密切相关的两个蛋白家族。GKN蛋白具有调节胃黏膜上皮细胞增殖和凋亡、维持胃黏膜完整和促进受损黏膜修复的作用;TFF蛋白具有促进损伤黏膜修复... 详细信息

gastrokine(GKN)和三叶因子家族(TFF)是在正常胃黏膜上皮细胞中大量特异性表达且结构和功能密切相关的两个蛋白家族。GKN蛋白具有调节胃黏膜上皮细胞增殖和凋亡、维持胃黏膜完整和促进受损黏膜修复的作用;TFF蛋白具有促进损伤黏膜修复以及影响细胞运动、黏附、凋亡等作用。两者在胃癌组织和细胞株中表达异常,可能在胃癌的发生中起重要作用,为胃癌特异性抑癌基因。本文就GKN、TFF蛋白与胃癌的关系作一综述。

关键词： gastrokine 三叶因子家族胃肿瘤基因肿瘤抑制