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Influence of gastric inhibitory polypeptide on pentagastrinstimulated gastric acid secretion in patients with type 2 diabetes and healthy controls

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World Journal of Gastroenterology 2006年第12期12卷 1874-1880页

作者： Juris J Meier Michael A Nauck Bartholomaeus Kask Jens J Hoist Carolyn F Deacon Wolfgang E Schmidt Baptist Gallwitz Department of Medicine I St. Josef-HospitalRuhr-University Bochum Germany Diabeteszentrum Bad Lauterberg Germany Department of Medical Physiology The Panum Institute University of Copenhagen Denmark Department of Medicine IV Eberhard-Karls- University Ttibingen Germany

AIM： gastric inhibitory polypeptide is secreted from intestinal K-cells in response to nutrient ingestion and acts as an incretin hormone in human physiology. While animal experiments suggested a role for GIP as an inhibitor of gastric secretion, the GIP effects on gastric acid output in humans are still controversial. METHODS： Pentagastrin was administered at an infusion rate of 1 μg . kg^-1 . h^-1 over 300 min in 8 patients with type 2 diabetes （2 female, 6 male, 54± 10 years, BMI 30.5 ± 2.2 kg/m^2; no history of autonomic neuropathy） and 8 healthy subjects （2/6, 46 ± 6 years., 28.9 ± 5.3 kg/ m^2）. A hyperglycaemic clamp （140 mg/dl） was performed over 240 min. Placebo, GIP at a physiological dose （1 pmol . kg^-1 . min^-1）, and GIP at a pharmacological dose （4 mol . kg^-1 . min^-1） were administered over 60 min each. Boluses of placebo, 20 pmol GIP/kg, and 80 pmol GIP/kg were injected intravenously at the beginning of each infusion period, respectively. gastric volume, acid and chloride output were analysed in 15-min intervals. Capillary and venous blood samples were drawn for the determination of glucose and total GIP. Statistics were carried out by repeated-measures ANOVA and one-way ANOVA. RESULTS： Plasma glucose concentrations during the hyperglycaemic clamp experiments were not different between patients with type 2 diabetes and controls. Steady-state GIP plasma levels were 61 ±8 and 79 ± 12 pmol/I during the low-dose and 327±35 and 327± 17 pmol/I during the high-dose infusion of GIP, in healthy control subjects and in patients with type 2 diabetes, respectively （P= 0.23 and p 0.99）. Pentagastrin markedly increased gastric acid and chloride secretion （P〈 0.001）. There were no significant differences in the rates of gastric acid or chloride output between the experimental periods with placebo or any dose of GIP. The temporal patterns of gastric acid and chloride secretion were similar in patients with type 2 diabetes and healthy controls （P= 0.86 and P= 0.6

关键词： gastric inhibitory polypeptide gastric acid secretion Type 2 diabetes Hyperglycemic clamp Pentagastrin-stimulated acid secretion

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Construction and Expression of Recombinant Ghrelin Plasmid and Effects on Growth Performance and gastric acid secretion of Rats

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Animal Husbandry and Feed Science 2013年第5期5卷 227-231页

作者： DU Gai-mei JIANG Jia-jin YAN Wen-mei HU Zhi-hua LUO Bi-ping ZHANG Yu-hong ZHANG Miao LI Liu-an LIU Mao-jun Department of Animal Science and Technology Jinling Technology Institution Department of Animal Science Tianjin Agricultural University Institute of Veterinary Medicine Jiangsu Academy of Agricultural Sciences /Key Laboratory of Veterinary Bilological Engineering and TechnologyMinistry of Agriculture /National Center for Engineering Research of Veterinary Bio-products

The paper was to study construction and expression of rGhrelin （pcDNA3 -Ghrelin） and its effect on growth performance and gastric acid secretion of rats. Ghrelin amplified from gastric mucosa of weaned piglets was cloned into the expression vector pcDNA3 to get recombinant plasmid pcDNA3 - Ghrelin. Twelve weaning rats were randomly divided into two groups, six rats each group. The rats in each treatment group were individually injected with 100pg of naked plasmid pcDNA3 -Ghrelin, and the rats in control group were injected with empty plasmid. The weights and feed consumption of rats were measured after injection for 7, 14 and 29 d, respectively. The rats were sacrificed at the end of the experiment, and their stomach was separated and weighed, the pH value of gastric juice was measured as well. The results showed that the average daily gain of rats at 7 and 29 d were significantly higher than that in control group, respectively （P〈0.05）, and feed consumption did not have significant chan- ges; the feed meat ratio of rats in the treatment groups was significantly lower than that in control group （ P 〈0.05） ; the gastric relative weight and gastric weight did not change significantly, while the pH value of gastric juice of rats in treatment groups was significantly lower than that in control group （P 〈 0.05）. This indicated that after transfected expression of muscle tissue, ghrelin played an important regulatory role in growth and gastric acid secretion of rats.

关键词： Recombinant Ghrelin plasmid Expression gastric growth development gastric acid secretion Rat

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The effects of microinjection of sodium hydrogen sulfide into dorsal motor nucleus of the vagus on gastric acid secretion in rats

The effects of microinjection of sodium hydrogen sulfide int...

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中国生理学会第24届全国会员代表大会暨生理学学术大会

作者：孙洪兆 Yu Kai-Hua Ai Hong-Bin College of Life Science Qilu Normal University College of Life Science Shandong Normal University

Background:Physiological functions of endogenous hydrogen sulfide(H2S),as a neuromodulator in central nervous system attract much attention *** studies clue to us that H2S plays important roles in regulating gastric function within the dorsal motor nucleus of the vagus(DMV).The aim of this study is to investigate the effects of microinjection of sodium hydrogen sulfide(Na HS),exogenous hydrogen sulfide donor,on gastric acid secretion in *** and methods:Esophagus perfusion was used to collect the gastric *** titratable H+quantum was ***:Microinjection of Na HS(0.04mol/L and 0.08mol/L respectively)into the DMV significantly promoted gastric acid secretion in Wistar rats,but microinjection of physiological saline(PS,0.1ul)at the same position and the same volume did not change the H+quantity of gastric juice obviously(n=7,P>0.05).At the concentration of0.04 mol/L(0.1ul,n=6),H+quantity of gastric juice increased from 865.20±102.22(20 min of before microinjection)to 1872.10±136.80(the first 20 min of after microinjection,Pgastric juice increased from 963.6±78.96(20 min of before microinjection)to 1985.10±104.47(the first 20 min of after microinjection,Pgastric acid secretion obviously.

关键词： dorsal motor nucleus of the vagus gastric acid secretion hydrogen sulfide

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gastric Mucosal Blood Flow and acid secretion in Rats

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Journal of Medical Colleges of PLA(China) 1989年第3期4卷 259-262页

作者：李兆申周岱云许国铭 Department of Gastroenterology Changhai Hospital Second Military Medical University Shanghai

The relationship between gastric mucosal blood flow (GMBF) and gastric acid secretionwere studied in rats by using secretory stimulant (pentagastrin)and inhibitor(cimetidine). GMBFwas measured by Laser Doppler flowmetry (LDF) and gastric mucosal pH determined by microglasspH electrode. GMBF increased and gastric mucosal pH decreased significantly after intravenous injec-tion of 6μg/kg of pentagastrin; nevertheless GMBF decreased and gastric mucosal pH increasedmarkedly after intravenous administration of 100mg/kg of cimetidine. This indicates that pentagastrincan increase GMBF and gastric acid secretion, and cimetidine can decrease GMBF and gastric acidsecretion in rats, proving the close relationship between GMBF and gastric acid secretion in rats.

关键词： gastric mucosal blood flow gastric acid secretion H_2 antagonist Laser Doppler flowmetry

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Effect of Lanthanum on acid secretion from Isolated Mouse Stomach in Vitro

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Journal of Rare Earths 2004年第3期22卷 427-430页

作者：徐项桂夏洪涛芮光胡翠英袁福根 Department of Biology Department

To explore the effect and the mechanism of La^(3+) on gastric acid secretion (GAS) of isolated mouse stomach with perfused lumen, 12 cm H_2O column intragastric pressure-provided, whole stomach preparations from mice were incubated in buffer at 37 ℃ in vitro, and perfusate was measured for pH with a pHS-3 type pH meter. The results show that La^(3+) (0.41～820×10^(-6) mol·L^(-1)) significantly promotes GAS in a concentration-dependant manner. Proglutamine, a blocker of gastrin receptor, potently inhibits GAS, and it may block the promotive effect of La^(3+) on GAS, and this effect increases with the increase of proglutamin concentration. Cimetidine, a blocker of histamine H_2 receptor, also potently inhibits GAS, and blocks the promotive effect of La^(3+) on GAS in the same manner with proglutamine. These results suggest that La^(3+) promotes GAS in isolated stomach possibly by stimulating the releases of gastrin from G cell and Histamine from ECL cell or by activating the gastrin receptors and Histamine H_2 receptors on the parietal cell, thereby accelerating the acid secretion of parietal cells in stomach.

关键词： physiology La^(3+) ion isolated mouse stomach gastric acid secretion proglutamine cimetidine rare earths

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Direct measurement of gastric H^+/K^+-ATPase activities in patients with or without Helicobacter pylori-associated chronic gastritis

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World Journal of Gastroenterology 2005年第23期11卷 3514-3517页

作者： Duangporn Thong-Ngam Pisit Tangkijvanich Pichet Sampatanukul Paungpayom Prichakas Varocha Mahachai Piyaratana Tosukowong Department of Physiology Department of Biochemistry Department of Pathology Department of Nuclear Medicine Department of Medicine

AIM:The role of Helicobacter pylori (H pylori) infection in gastric acid secretion of patients with chronic gastritis remains controversial. This study was designed to elucidate the effect of H pylori on H+/K+-ATPase activities in gastric biopsy specimens. METHODS: Eighty-two patients with chronic gastritis who had undergone upper endoscopy were included in this study. H pylori infection was confirmed by rapid urease test and histology. gastric H+/K+-ATPase activities and serum gastrin concentrations were measured by an enzymatic method and radioimmunoassay, respectively. For those patients who received triple therapy for eradicating H pylori, changes in the activity of gastric H+/K+-ATPase and serum gastrin levels were also measured. RESULTS: The mean gastric H+/K+-ATPase activity in Hpylori-positive group (42 patients) was slightly higher than that in Hpylori-negative group (29 patients) (169.65±52.9 and 161.38±43.85nmol P/(mg·h),respectively, P=0.301). After eradication of H pylori, the gastric H+/K+-ATPase activities slightly decreased compared to prior therapy (165.03±59.50 and 158.42±38.93 nmol P/(mg·h), respectively, P=0.805). The mean basal gastrin concentration was slightly higher in H pylori-positive patients than in H pylori-negative patients (87.92±39.65 pg/mL vs75.04±42.57 pg/mL, P= 0.228). The gastrin levels fell significantly after the eradication of H pylori. (Before treatment 87.00±30.78 pg/mL, after treatment 64.73±18.96 pg/mL, P=0.015). CONCLUSION: gastric H+/K+-ATPase activities are not associated with H pylori status in patients with chronic gastritis.

关键词： H pylori gastric acid secretion Chronic gastritis

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