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Total Triterpenoids from ganoderma Lucidum Suppresses Prostate Cancer Cell Growth by inducing Growth arrest and apoptosis

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Journal of Huazhong University of Science and Technology(Medical Sciences) 2015年第5期35卷 736-741页

作者：王涛谢子平黄展森李浩韦安阳狄金明肖恒军张志刚蔡柳洪陶欣齐涛陈地灵陈俊 Department of Urology Medical Center for Overseas PatientsNanfang HospitalSouthern Medical University Department of Urology Longjiang Hospital of Shunde District in Foshan City Department of Andrology Renmin HospitalHubei University of Medicine Department of Infertility and Sexual Medicine the Third Affiliated Hospital of Sun Yat-sen University Department of Urology the Third Affiliated Hospital of Sun Yat-sen University Department of Pathology the Third Affiliated Hospital of Sun Yat-sen University Center for Reproductive Medicine the Third Affiliated Hospital of Sun Yat-sen University State Key Laboratory of Applied Microbiology South ChinaGuangdong Institute of Microbiology

In this study,one immortalized human normal prostatic epithelial cell line（BPH） and four human prostate cancer cell lines（LNCa P,22Rv1,PC-3,and DU-145） were treated with ganoderma Lucidum triterpenoids（GLT） at different doses and for different time periods. Cell viability,apoptosis,and cell cycle were analyzed using flow cytometry and chemical assays. Gene expression and binding to DNA were assessed using real-time PCR and Western blotting. It was found that GLT dose-dependently inhibited prostate cancer cell growth through induction of apoptosis and cell cycle arrest at G1 phase. GLT-induced apoptosis was due to activation of caspases-9 and-3 and turning on the downstream apoptotic events. GLT-induced cell cycle arrest（mainly G1 arrest） was due to up-regulation of p21 expression at the early time and down-regulation of cyclin-dependent kinase 4（CDK4） and E2F1 expression at the late time. These findings demonstrate that GLT suppresses prostate cancer cell growth by inducing growth arrest and apoptosis,which might suggest that GLT or ganoderma Lucidum could be used as a potential therapeutic drug for prostate cancer.

关键词： ganoderma apoptosis cytometry arrest assessed caspases inhibited inducing viability downstream

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