检索结果-南通市图书馆

Effect of whole methanolic extract of galium verum on AGO cell line

Toxicology Communications 2022年第2期4卷 35-40页

作者： Sanaz Pashapour Masoumeh Heshmati Zahra Mousavi Somayeh Esmaeili Department of Pharmacology and Toxicology Faculty of Pharmacy and Pharmaceutical SciencesTehran Medical SciencesIslamic Azad UniversityTehran 009821Iran Department of Molecular and Cellular Sciences Faculty of Advanced Science and TechnologyTehran Medical SciencesIslamic Azad UniversityTehran 009821Iran Traditional Medicine and Materia Medica Research Center Department of Traditional PharmacySchool of Traditional MedicineShahid Beheshti University of Medical SciencesTehran 009821Iran

Objectives:Although many studies have suggested the anticancer properties of galium verum,there is still no accurate information regarding its side effects on normal ***,this study aimed to investigate the dual effects of the whole galium verummethanolic extract on the normal human fibroblast cell line(AGO)cell line at different ***:The cell line was randomly divided into a control group and groups exposed to concentrations of 12.5 to 400μg/*** was performed by the maceration *** addition,the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide(MTT)method was applied to measure cytotoxicity and flow ***,BCL2 associated X(BAX)andB-cell lymphoma 2(BCL2)genes were expressed by the real-time polymerase chain reaction to evaluate apoptosis and reactive oxygen species(ROS).Finally,data were compared between groups using a one-way analysis of ***:A significant reduction was observed in the cell viability of 90%at a concentration of 400μg/mL compared to the *** comparison,a significant increase was reported in cell viability at concentrations of 25-200μg/mL(P<0.0001).Furthermore,there was a significant 2.87-time increase in apoptosis compared to the control group(P<0.0001),but no significant differences were reported in cellular *** increased significantly by 5.7 times(P<0.05),and a significant 80-fold increase was found in the BAX/BCL2gene ratio(P<0.05).Conclusion:The whole methanolic extract could lower the viability of human fibroblasts at 400μg/mL and more by increasing apoptosis,thereby increasing BAX/BCL2gene expression and ROS ***,the extract exerted an increased effect on cell viability in a concentration-dependent manner on AGO and increased cell growth at concentrations less than 400μg/mL,highlighting different effects of the whole extract on the AGO cell line.

关键词： galium verum AGO Apoptosis ROS BAX gene BCL2 gene

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Therapeutic effects of the galium verum

引用

Food and Health 2022年第3期4卷 5-8页

作者： Abbas Zabihi Sanaz Pashapour Department of Biology Faculty of Basic SciencesIslamic Azad UniversityHamedan BranchHamedan 009881Iran Department of Pharmacology and Toxicology Faculty of Pharmacy and Pharmaceutical SciencesTehran Medical SciencesIslamic Azad UniversityTehran 009821Iran.

Medicinal plants apply become a popular cure between patients with various diseases *** of this review study of investigating of therapeutic effects of galium *** the current review study,PubMed,Scopus,and Google Scholar were searched to provide information have shown the therapeutic effects of *** plant,is famous,as yellow *** plant belongs to the Rubiaceae *** of *** plant represents that the plant included,chemical therapeutic *** compounds have been isolated from the galium species plant so *** have been traditionally applied for the cure of many *** former pharmacological researches represented that the *** plant possessed antioxidant effect,effects on the central nervous system,antimicrobial effects,endocrine effect,antihaemolytic activity,cytotoxic effects,etc.

关键词： galium verum medicinal plants cancers therapeutic effects

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：