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Role of Eclipta prostrata extract in improving spatial learning and memory deficits in D-galactose-induced aging in rats

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Journal of Traditional Chinese Medicine 2019年第5期39卷 649-657页

作者： Xia Xichao Yu Ruixue Wang Xiaowei Wei Mengwei Yi Li Wang Aimei Ma Yuhong Zhang Junfeng Ji Zhaohui Li Yuan Wang Qiong Medicine College of Pingdingshan University Pingdingshan 467000China Basic Medicine College Nanyang Medical UniversityNanyang 473061China

OBJECTIVE: To investigate the role of Eclipta prostrata (E. prostrata) extract in improving spatial learning and memory deficits in D-galactose-induced aging in rats. METHODS: Rats were divided into five groups, with 10 animals in each group. Aging rats were produced by treatment with 100 mg·kg-1·d-1 of D-galactose for 6 weeks. Rats in the E. prostrata treatment groups received an aqueous extract of E. prostrata orally at a concentration of 50, 100, or 200 mg·kg-1· d-1 for 3 weeks. Animals in both the normal and model groups were treated with similar volumes of saline. Spatial memory performance was measured using the Morris water maze. The mRNA levels and enzyme activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione reductase (GR) were analyzed using real- time quantitative PCR and spectrophotometry,respectively. The levels of induced nitric oxide synthase (iNOS), nitric oxide (NO), dopamine (DA), norepinephrine (NE), and serotonin (5-HT) were determined using enzyme-linked immunosorbent assay and spectrophotometry. RESULTS: Compared with the normal group, rats in the D-galactose-treated model group exhibited significant memory loss. There was severe damage to the hippocampal CA1 area, and expression levels of SOD, CAT, GPx, and GR were significantly decreased in the model group compared with the normal group. In the model group, levels of iNOS and NO were significantly increased compared with the normal group. However, treatment with E. prostrata extract reversed the conditions caused by D-galactose- induced aging, especially in the groups with higher treatment concentrations. Compared with the normal group, the levels of DA, NE, and 5-HT were significantly lower in the D-galactose-treated model group. In the E. prostrata extract-treated groups, however, there was a dose-dependent upregulation of DA, NE, and 5-HT expression. CONCLUSION: Our results suggest that administration of E. prostrata extract can result in an

关键词： Eclipta galactose Spatial learning Memory disorders Antioxidants

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Study on the dual-frequency oscillations in galactose-BZ system

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Chinese Journal of Chemistry 1997年第1期15卷 25-30页

作者： LI, He-XingDepartment of Chemistry, Shanghai Normal University,Shanghai 200234, China Department of Chemistry Shanghai Normal University Shanghai 900234 China

Three types of dual-frequency oscillations in KBrO3-galactose-acetone-MnSO4-H2SO4 have been observed in a batch reactor depending on the initial concentration of H2SO4 or Br-. Similar phenomena were also observed when galactose was replaced by other aldosugars such as glucose, arabinose, lactose and maltose. However no dual-frequency oscillation has been found when ketosugar like fructose was used instead of galactose as the substrate. The roles of acidity, bromide ion and acetone in dual-frequency oscillations have been analyzed. The mechanism of dual-frequency oscillations has been discussed.

关键词： galactose dual-frequency oscillations acidity bromide

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Curcumin delivery nanoparticles based on Maillard reaction of Haematococcus pluvialis protein/galactose for alleviating acute alcoholic liver damage

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Food Science and Human Wellness 2024年第5期13卷 2629-2641页

作者： Xinyi Liu Yukun Song Shasha Cheng Mingqian Tan School of Food Science and Technology Dalian Polytechnic UniversityDalian 116034China National Engineering Research Center of Seafood Dalian 116034China

The aim of this study is to investigate the feasibility of Maillard reaction products of Haematococcus pluvialis protein and galactose(HPP-GAL)for improving the bioactivities of curcumin(CUR)for alleviating alcoholic liver *** was embedded into HPP-GAL nanoparticles by the self-assembly of hydrogen bonding and hydrophobic interaction with the particle size around 200 ***-GAL enhanced the encapsulation efficiency and loading amount of CUR with the value of(89.21±0.33)%and(0.500±0.004)%,*** stabilities of CUR under strong acid,salt ion stability and ultraviolet irradiation conditions were improved by the ***-GAL-CUR nanoparticles exhibited excellent concentration-dependent in vitro antioxidant activities including DPPH and ABTS scavenging rates,and better protective effect on CUR against gastric acid environment as well as longer release of CUR in simulated intestinal *** addition,the HPPGAL-CUR delivery system possessed liver targeting property due to the existence of GAL,which could effectively alleviate the alcohol-induced liver damage and the inflammation indexes by inhibiting the oxidative ***,HPP-GAL-CUR nanoparticles might be a potential candidate system for the prevention of alcoholic liver damage in the future.

关键词： Haematococcus pluvialis protein galactose Curcumin nanocarrier Maillard reaction Alcoholic liver damage Liver targeting

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Multianalyte Biosensors for the Simultaneous Determination of Glucose and galactose Based on Thin Film Electrodes

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Chinese Chemical Letters 2004年第3期15卷 322-325页

作者： NengQinJIA ZongRangZHANG JiangZhongZHU GuoXiongZHANG DepartmentofChemistry ShanghaiNormalUniversityShanghai200234 StateKeyLabofTransducerTechnology ShanghaiInstituteofMetallurgyChineseAcademyofSciencesShanghai200050

A multianalyte biosensor for the simultaneous determination of glucose and galactose was developed by immobilizing glucose oxidase (GOD) and galactose oxidase (GAO) on Nafion-modified thin film platinum disk electrodes. The dual Pt working electrodes with disk shape and the surrounding ring shaped counter electrode were fabricated by thin film technology, which were integrated onto the same microchip. The response of the designed biosensor for glucose and galactose were linear up to 6.0 mmol/L and 3.5 mmol/L with sensitivities of 0.3 mA/mmol/L and 0.12 mA/mmol/L, respectively. No cross-talking effect was observed.

关键词： Multianalyte biosensor glucoe galactose Nafion thin film technology.

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Erratum on “galactose functionalized diketopyrrolopyrrole as NIR fluorescent probes for lectin detection and HepG2 cell targeting based on aggregation-induced emission mechanism” [Sci. China Chem., 2018, 61: 898–908]

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Science China Chemistry 2018年第12期61卷 1637-1638页

作者： YANDi Hang Xiaolei Cai Jian Wang Tao Jiang Jianli Hua Bin Liu Key Laboratory for Advanced Materials & Institute of Fine Chemicals East China University of Science and Technology Department of Chemical and Biomolecular Engineering National University of Singapore

In a recent publication [1],there is a mistake in Scheme 1,in which a propargyl alpha-mannoside was drawn on the arrow in the synthetic step from compounds 8/9 to 10/11. The correct structure should be a propargyl bet... 详细信息

关键词： galactose functionalized diketopyrrolopyrrole NIR

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Differing Roles for Clostridium acetobutylicum’s galactose Utilization Pathways

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Advances in Microbiology 2013年第6期3卷 490-497页

作者： C. J. Sund M. D. Servinsky E. S. Gerlach US Army Research Laboratory Biotechnology Branch Adelphi USA

There has been a surge of interest in acetone-butanol-ethanol fermentations of Clostridium acetobutylicum due to its capacity to ferment many carbohydrates found in biomass. This metabolic diversity makes it a promising candidate for conversion of inexpensive, heterogeneous carbohydrate feedstocks to biofuels. galactose is present in many such feedstocks due to its incorporation in plant cell walls. C. acetobutylicum encodes two galactose utilization pathways, the Leloir (LP) and the tagatose-6-P (T6P), and a previous study indicated genes for these pathways was differentially regulated during growth on galactose and lactose. In the current study we utilized quantitative PCR to further investigate gene expression levels and to show both pathways which were subject to carbon catabolite repression. During growth on galactose, mRNA for galactose-6-P isomerase from the T6P was induced to a greater extent than mRNA for glactokinase, the first enzyme in the LP. The galactose-6-P isomerase mRNAs were also more abundant than galactokinase mRNAs during growth on galactose. Analysis of theoretical ATP requirements to generate essential precursor metabolites indicated: 1) the LP is more efficient at generating upper glycolytic intermediates, 2) the T6P is more efficient at forming ATP, lower glycolytic intermediates and TCA cycle intermediates, 3) a combination of the two pathways is most efficient for forming precursor metabolites found in the pentose phosphate pathway. From this it can be suggested that the two pathways have different roles in the organism with the T6P generating most ATP and precursor metabolites and the LP providing upper glycolytic metabolites.

关键词： Clostridium acetobutylicum galactose Lactose Tagatose Leloir Pathway Fermentation

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Determination of Total galactose from Dried Blood Spots—Extensive Assay Evaluation of a CE-Marked Test-Kit

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Journal of Analytical Sciences, Methods and Instrumentation 2013年第3期3卷 163-166页

作者： Ralph Fingerhut Toni Torresani Swiss Newborn Screening Laboratory Children’s Research Center (CRC) University Children’s Hospital Zurich Switzerland.

Most newborn screening laboratories use CE-marked or FDA-approved test-kits, like in routine clinical chemistry. National regulations require only minimal evaluation from the customer, if the test-kits are used as specified by the manufacturer. The microtiter-based kit-concept is often based on the perception, that the laboratory always processes whole microtiter plates. However, in the daily routine, this is rather a rare exception, which leads to much higher costs per newborn, compared to the costs per assay in the test-kits. In addition the amount of wasted resources is quite high. Performance of the Neonatal Total galactose kit from Perkin Elmer was tested. We have determined specificity, limit of detection (LOD), limit of quantitation (LOQ), intra and inter assay variation, recovery, stability of measuring signal and reagents. Results were also compared with the Astoria Pacific Spot Check System. In addition, we had (by chance) the opportunity to test 2 kits, which were already expired for more than 3 years. LOD was 165 - 306 μmol/L and LOQ 475 - 703 μmol/L, depending on the definition of LOD/LOQ. Mean recovery was 112.8%, intra assay CVs were 11.3, 7.3, 4.0, and 3.0, and inter assay CVs 28.7, 15.9, 7.8, and 9.3, at 220, 590, 1200, and 2060 μmol/L respectively. Reconstituted and mixed reagents must be used within some hours, and were unstable even if stored at -20℃. However, if the reconstituted galactose substrate reagent and galactose oxidase reagent were only mixed according to the daily requirements, and the rest stored separately at -20℃, they were stable for at least 12 days. The performance of the expired test-kits did not differ from the others. The performance of the Total galactose kit is comparable to other tests used for newborn screening. However, we could significantly reduce the costs per newborn and reduce unnecessary production of waste, by thorough validation and modification of the assay procedures.

关键词： Newborn Screening galactosemia Total galactose

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Newborn screening for galactosemia:a 30-year single center experience

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World Journal of Pediatrics 2015年第2期11卷 160-164页

作者： Francesco Porta Severo Pagliardini Veronica Pagliardini Alberto Ponzone Marco Spada Department of Pediatrics University of TorinoTorinoItaly

Background:galactosemia due to complete or near-complete galactose-l-phosphate uridyltransferase(GALT)deficiency was the first disorder added to the pioneering newborn screening panel besides *** the last 50 years,many criticisms have been focused on the opportunity of its ***,long-term single center experiences with this issue are generally ***:We reviewed the outcome of newborn screening for hypergalactosemia performed at our department since 1982 and the correspondent long-term clinical ***:Among 1123909 newborns screened for hypergalactosemia,33 showed abnormal results confirmed at second tier *** patients were affected with classic galactosemia,8 partial GALT deficiency,3 severe galactokinase deficiency,7 transient galactosemia,one congenital porto-systemic shunt,and one glucose transporter 2 *** neonatal liver failure in the late first week of life(5.8±1.1 days)unavoidably complicated the clinical course of classic galactosemia,unless in three second-born siblings treated on the basis of presumptive diagnosis immediately after newborn screening sample collection on day *** early treatment and longterm steadily normal peripheral blood galactose,77%of patients with severe GALT deficiency present mild to severe intellectual *** patients with partial GALT deficiency showed normal intellectual development on a regular diet,as well as patients with galactokinase deficiency under ***:Availability of screening results within the fifth day after birth would allow the prevention of acute decompensation in classic galactosemia.A systematic diagnostic work-up in all positive newborns is essential to unravel the etiology of hypergalactosemia.

关键词： galactose galactosemia liver failure mental retardation newborn screening

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半乳糖修饰人血清白蛋白的质谱分析

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分析测试学报 2004年第Z1期23卷 37-38页

作者：蔡春蔡康荣广东医学院广东湛江524023

　　研究发现,存在于哺乳动物肝细胞膜上的血浆无唾液酸糖蛋白受体(asialoglycoprotein receptor,ASGP-R)是一种肝结合蛋白(hepatic binding protein,HBP),能专一性识别分子末端带有半乳糖残基的糖蛋白并与之结合,定向转到肝细胞内的溶... 详细信息

　　研究发现,存在于哺乳动物肝细胞膜上的血浆无唾液酸糖蛋白受体(asialoglycoprotein receptor,ASGP-R)是一种肝结合蛋白(hepatic binding protein,HBP),能专一性识别分子末端带有半乳糖残基的糖蛋白并与之结合,定向转到肝细胞内的溶酶体进行代谢[1],针对ASGP-R作为靶点的肝靶向药物载体半乳糖化人血白蛋白偶合物(galactosyl-neoglycoalbumin,NGA),已引起药物研究工作者的极大重视[2,3].将药物与带有半乳糖残基的蛋白偶合,可提高药物的肝靶向性,提高治疗指数,减少非靶器官的毒副作用.……

关键词： MALDI-TOF-MS Neoglycoalbumin Asialoglycoprotein receptor galactose

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Ligand dose-dependent activation of signaling pathways through the gustatory receptor NlGr11 linked to feeding efficacy in Nilaparvata lugens

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Insect Science 2023年第5期30卷 1420-1430页

作者： Wei-Wen Chen Kai Lin Jun Lv Qin Su Meng-Yi Zhang Kui Kang Wen-Qing Zhang State Key Laboratory of Biocontrol and School of Life Sciences Sun Yat-sen UniversityGuangzhouChina College of Biology and Agriculture Zunyi Normal UniversityZunyiGuizhou ProvinceChina

Insects often face both conditions with sufficient nutrients and conditions of undernutrition in the *** gustatory receptors,insects sense nutrients and regulate their physiological functions such as feeding and ***,it remains unclear whether signaling pathways activated by gustatory receptors depend on the concentration of nutrients and whether the difference in signaling pathways directly affects insects’physiological ***,we found that a sugar gustatory receptor,NlGr11,from the brown planthopper(BPH),Nilaparvata lugens,activated G protein-coupled signaling and ionotropic pathways when bound to high galactose *** subsequently demonstrated longer feeding times,feeding loads,and higher vitellogenin(NlVg)expression than BPHs exposed to high galactose concentrations,which only activated the ionotropic *** the first time,our findings link plant nutrient conditions,signaling pathways activated by nutrients,and their gustatory receptors,and nutrient dose-dependent feeding efficacy and vitellogenin(Vg)expression in an *** will help us to better understand the molecular mechanism for insect feeding strategies on plants at different stages of nutritional conditions.

关键词： feeding efficacy galactose G protein-coupled signaling pathway insect gustatory receptor ionotropic pathway

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