检索结果-南通市图书馆

首届长三角科技论坛——长三角生物医药发展论坛

作者：龚兴国钟文涛谢捷周远 nstitute of Biomacromolecule & Enzyme Engineering College of Life SciencesZhejiang UniversityHangzhou310027China Institute of Biomacromolecule & Enzyme Engineering College of Life SciencesZhejiang UniversityHangzhou310027China

v-Src is a non-receptor protein tyrosine kinase,which is involved in many signal transduct-ion pathways and closely related to the activation and/or development of *** we presentedthe expression,purification and initial characterization of gst-fusion v-Src in plsmid pLysS from abaterial expression *** culture conditions were examined in the indution of IPTG,andGSH affinity chromatography was used to purified v-Src *** the versatile sub-strate poly-(Glu:Tyr)4:1,ELISA was employed to ditermine the phosphorylation kinase activity of *** strategy seems to be more promise than the insect cell system or other eukaryotic systemsemployed in earlier Src expression.

关键词： v-src gst-fusion inclusion body orthogonalization protein tyrosine Kinase

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Expression, purification, and bioactivity of gst-fused v-Src from a bacterial expression system

引用

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2006年第1期7卷 13-19页

作者：龚兴国纪静谢捷周远张俊彦钟文涛 Institute of Biomacromolecule and Enzyme Engineering School of Life Sciences Zhejiang UniversityHangzhou 310027 China

v-Src is a non-receptor protein tyrosine kinase involved in many signal transduction pathways and closely related to the activation and development of cancers. We present here the expression, purification, and bioactivity of a gst (glutathione S-transferase)-fused v-Src from a bacterial expression system. Different culture conditions were examined in an isopropyl β-D-thiogalactopyranoside (IPTG)-regulated expression, and the fused protein was purified using GSH (glutathione) affinity chromatography. ELISA (enzyme-linked immunosorbent assay) was employed to determine the phosphorylation kinase activity of the gst-fused v-Src. This strategy seems to be more promising than the insect cell system or other eukaryotic systems em- ployed in earlier Src expression.

关键词： v-Src gst-fusion Inclusion body Orthogonalization Protein tyrosine kinase

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：