检索结果-南通市图书馆

glutamate triggers the expression of functional ionotropic and metabotropic glutamate receptors in mast cells

Cellular & Molecular Immunology 2021年第10期18卷 2383-2392页

作者： Md Abdul Alim Mirjana Grujic Paul W.Ackerman Per Kristiansson Pernilla Eliasson Magnus Peterson Gunnar Pejler Department of Public Health and Caring Sciences General Medicine and Preventive MedicineUppsala UniversityUppsalaSweden Department of Medical Biochemistry and Microbiology Uppsala UniversityUppsalaSweden Department of Molecular Medicine and Surgery Karolinska InstituteStockholmSweden Department of Orthopedics and Sports Medicine Linköping UniversityLinköpingSweden Academic Primary Health Care UppsalaSweden Department of Anatomy Physiology and BiochemistrySwedish University of Agricultural SciencesUppsalaSweden

Mast cells are emerging as players in the communication between peripheral nerve endings and cells of the immune system.However,it is not clear the mechanism by which mast cells communicate with peripheral nerves.We previously found that mast cells located within healing tendons can express glutamate receptors,raising the possibility that mast cells may be sensitive to glutamate signaling.To evaluate this hypothesis,we stimulated primary mast cells with glutamate and showed that glutamate induced the profound upregulation of a panel of glutamate receptors of both the ionotropic type(NMDAR1,NMDAR2A,and NMDAR2B)and the metabotropic type(mGluR2 and mGluR7)at both the mRNA and protein levels.The binding of glutamate to glutamate receptors on the mast cell surface was confirmed.Further,glutamate had extensive effects on gene expression in the mast cells,including the upregulation of proinflammatory components such as IL-6 and CCL2.glutamate also induced the upregulation of transcription factors,including Egr2,Egr3 and,in particular,FosB.The extensive induction of FosB was confirmed by immunofluorescence assessment.glutamate receptor antagonists abrogated the responses of the mast cells to glutamate,supporting the supposition of a functional glutamate–glutamate receptor axis in mast cells.Finally,we provide in vivo evidence supporting a functional glutamate–glutamate receptor axis in the mast cells of injured tendons.Together,these findings establish glutamate as an effector of mast cell function,thereby introducing a novel principle for how cells in the immune system can communicate with nerve cells.

关键词： glutamate glutamate receptors Mast cells NMDA receptors Tryptase

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

glutamate in cancers:from metabolism to signaling

引用

The Journal of Biomedical Research 2020年第4期34卷 260-270页

作者： Haowei Yi Geoff Talmon Jing Wang Department of Genetics Cell Biology and AnatomyUniversity of Nebraska Medical CenterOmahaNE 68198USA. Department of Pathology and Microbiology University of Nebraska Medical CenterOmahaNE 68198USA. Eppley Institute for Research in Cancer and Allied Diseases University of Nebraska Medical CenterOmahaNE 68198USA.

Glutamine and glutamate are major bioenergy substrates for normal and cancer cell growth.Cancer cells need more biofuel than normal tissues for energy supply,anti-oxidation activity and biomass production.Genes related to metabolic chains in many cancers are somehow mutated,which makes cancer cells more glutamate dependent.Meanwhile,glutamate is an excitatory neurotransmitter for conducting signals through binding with different types of receptors in central neuron system.Interestingly,increasing evidences have shown involvement of glutamate signaling,guided through their receptors,in human malignancy.Dysregulation of glutamate transporters,such as excitatory amino acid transporter and cystine/glutamate antiporter system,also generates excessive extracellular glutamate,which in turn,activates glutamate receptors on cancer cells and results in malignant growth.These features make glutamate an attractive target for anti-cancer drug development with some glutamate targeted but blood brain barrier impermeable anti-psychosis drugs under consideration.We discussed the relevant progressions and drawbacks in this field herein.

关键词： glutamate cancers metabolism glutamate receptors glutamate transporters

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Quantitative Autoradiographic Study on Receptor Regulation in the Basal Ganglia in Rat Model of Levodopa-induced Motor Complications

引用

Journal of Huazhong University of Science and Technology(Medical Sciences) 2009年第2期29卷 156-162页

作者：徐岩张振涛秦开蓉 Stella M.Papa 曹学兵 Department of Neurology Union HospitalTongji Medical CollegeHuazhong University of Science and Technology Department of Rehabilitation Tongji HospitalTongji Medical CollegeHuazhong University of Science and Technology Department of Neurology School of MedicineEmory University

In order to study neurotransmitter receptor regulation in the basal ganglia involved in the functional changes underlying levodopa-induced motor complications, quantitative autoradiography was used to observe receptor bindings of dopamine D1 and D2, N-methyl-D-aspartate （NMDA）, amino-3-hydroxy-5-methylisoxazole propionic acid （AMPA） and amino butyric acid （GABA） in the basal ganglia of rats that had unilateral nigrostriatal lesions and had been chronically treated with levodopa until motor complications developed. The rats were randomly assigned to three groups： normal, denervated and treatment-complicated groups. The results showed that response duration to levodopa became progressively shorter and abnormal involuntary movement （AIM） score was progressively increased during the course of levodopa treatment. Chronic treatment augmented D1 receptors more than denervation, and reduced D2 receptors that were also increased by dopamine denervation. Striatal NMDA receptors were substantially up-regulated in the treatment-complicated group. Levodopa treatment did not change receptors of nigral AMPA, pallidal GABA, and subthalamic GABA, which remained the same as that in denervation group. However, chronic treatment reversed the increase of nigral GABA receptors caused by the lesion. It was concluded that a shortening of response duration and AIM mimicked levodopa-induced motor complications of Parkinson＇s patients. These data suggested that up-regulation of dopamine D1 and NMDA receptors in the striatum leads to an imbalance of stimulation through the striatal output pathways, which is associated with levodopa-induced motor complications.

关键词： levodopa-induced motor complications Parkinson＇s disease glutamate receptors GABA receptors dopaminc receptors autoradiography

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Putative Nitrogen Sensing Systems in Higher Plants

引用

Journal of Integrative Plant Biology 2006年第8期48卷 873-888页

作者： Hon-Ming Lam Ying Ann Chiao Man-Wah Li Yuk-Kwong Yung Sang Ji Department of Biology Chinese University of Hong Kong Shatin Hong Kong Molecular Biotechnology Program Chinese University of Hong Kong Shatin Hong Kong

Nitrogen （N） metabolism is essential for the biosynthesis of vital biomolecules. N status thus exerts profound effects on plant growth and development, and must be closely monitored. In bacteria and fungi, a few sophisticated N sensing systems have been extensively studied. In animals, the ability to receive amino acid signals has evolved to become an integral part of the nervous coordination system. In this review, we will summarize recent developments in the search for putative N sensing systems in higher plants based on homologous systems in bacteria, fungi, and animals. Apparently, although plants have separated and diversified from other organisms during the evolution process, striking similarities can be found in their N sensing systems compared with those of their counterparts; however, our understanding of these systems is still incomplete. Significant modifications of the N sensing systems （including cross-talk with other signal transduction pathways） in higher plants may be a strategy of adaptation to their unique mode of life.

关键词： general amino acid control glutamate receptors His-Asp phosphorelay nitrogen metabolism nitrogen sensing PII, signal transduction.

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The Cardiovascular Inhibition of Hydrogen Sulfide in the Nucleus Tractus Solitarii of Anesthetized Rats is in Relation with KATP Channel Opening and glutamate Receptor Activation Mechanism

The Cardiovascular Inhibition of Hydrogen Sulfide in the Nuc...

引用

第八届海峡两岸心血管科学研讨会

作者： Wei QIAO~(1,2),Xiao-yun LI~1,Chen CHAI~2,Nong CAO~2,Yan LU~1 (1.Department of Clinical Laboratory,SanAiTang Hospital,Lanzhou 730030,China 2.Department of General surgery,First Hospital of Lanzhou University,Lanzhou 730000,China)

Objective Hydrogen sulfide(HS),the colorless gas with the smell of rotten eggs,is regarded as a novel gaseous signaling molecule of nervous,cardiovascular,and inflammatory systems.Accumulating evidence suggests that HS plays an important role in the central control of the cardiovascular system.However, the cardiovascular regulation of HS remains to be determined within the nucleus tractus solitarii(NTS). Therefore,our study aimed to investigate the possible physiological role of endogenous HS in modulating the cardiovascular effects in the NTS in anaesthetized and paralyzed rats.Methods the dosage effects of endogenous HS within NTS,32 anesthetized and paralyzed rats,which were randomized into 4 groups,received topically application of HS donor NaHS(2,20 and 200 pmol) or vehicle(100 nL,n=8).The cardiovascular functions of endogenous HS were further determined by increasing the content of endogenous HS by microinjection of SAM,the allostertic activator of CBS into NTS in 6 rats.As control,the vehicle(aCSF,100 nL) was injected into NTS in another 7 rats.Another rats were initially pretreated with HA,glibenclamide, kynurenic acid or vehicle(aCSF or 1%DMSO) 10 min before microinjection of 20 pmol NaHS into the NTS. The original tracings of BP and HR responses to intra - NTS NaHS were recorded for at least 60 min.Results the NTS caused the transient and dose - dependent hypotension and bradycardia(P<0.01),and subsequently the values gradually recovered to the basal level in about one minute.Injection of CBS allosteric activator S - ademetionine(SAM) into the NTS also produced significant decreases in BP(from 101±8 to 82±7 mmHg,P<0.01) and HR(from 469±16 to 449±14 bpm,P<0.01),which was similar to those of 20 pmol NaHS.Pretreatment with hydroxylamine or vehicle(aCSF or 1%DMSO) failed to affect the cardiovascular functions of intra - NTS NaHS.However,pretreatment with glibenclamide(by 78%and 79%) or kynurenic acid(59%and 53%) effectively eliminated the inhibitory effects of NaHS(20 pmol) on BP and HR respectively.Conclus

关键词： blood pressure heart rate hydrogen sulfide KATP channels glutamate receptors the nucleus tractus solitarii

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

HIV-1 Glycoprotein 120 Enhancement of N-Methyl-D-Aspartate NMDA Receptor-Mediated Excitatory Postsynaptic Currents: Implications for HIV-1-Associated Neural Injury

HIV-1 Glycoprotein 120 Enhancement of N-Methyl-D-Aspartate N...

引用

中国神经科学学会第十二届全国学术会议

作者： Yan Zhou Jianuo Liu Huangui Xiong Department of Pharmacology Guangxi Medical University Department of Pharmacology and Experimental Neuroscience University of Nebraska Medical Center

Objective Human immunodeficiency virus type 1(HIV-1) envelope glycoprotein 120(gp120) plays an important role in HIV-1-associated neuropathology. Multiple pathways have been proposed for gp120-induced neurotoxicity, amongst is the activation of N-Methyl-D-Aspartate receptors(NMDARs). It has been shown that gp120 causes neuronal injury and gp120 transgenic mice exhibit neurological similarity to humans infected with HIV-1, all of which can be blocked or attenuated by NMDAR antagonists. Several lines of evidence indicate the subtype and location of activated NMDARs are key determinants of the nature of NMDAR physiology.The present study is to examine the subtype and the location of NMDARs affected by gp120. Methods "blind"whole-cell patch recordings were used to study gp120 on subtype NMDARmediated EPSCs in the CA1 region of rat hippocampal slices. Results Our results showed bath application of gp120 increased both NR2 A-and NR2 B-mediated EPSCs possibly via a presynaptic mechanism, with much stronger effect on NR2 B-mediated EPSCs. In contrast, gp120 failed on enhancing AMPA receptor-mediated EPSCs. Ca imaging studies revealed that gp120 potentiated glutamate-induced increase of intracellular Ca concentration in rat hippocampal neuronal cultures which were blocked by a NMDAR antagonist, but not by an AMPA receptor antagonist, indicating gp120 induce Ca influx through NMDARs. Further investigation demonstrated that gp120 increased the EPSCs mediated by extrasynaptic NR2 BRs. Conclusion Taken together, these results demonstrate that gp120 interacts with both NR2 A and NR2 B subtypes of NMDARs with a predominant action on the extrasynaptic NR2 B, which may implicate a role for NR2 B in HIV-1-associated neuropathogenesis.

关键词： HIV Synaptic transmission CA1 glutamate receptors Neurodegeneration

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：