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genetic manipulation in higher organisms Ⅰ. GOLDFISH OVA AS MATERIALS OF OPERATION, mRNA MEDIATED ALTERATION OF THE LIVER SPECIFIC ISOZYMES

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Science in China,Ser.A 1977年第6期 803-806页

作者：牛满江童第周

Both crucian and carp egg-mRNAs were found to mediate the transformation of goldfish tail from the double （veil-shaped） to the single （fork-shaped）. Livers of these single tailed goldfish were tested for genetic change using lactate dehydrogenase isozymes （LDH） as marker. Electrophoresis of liver extracts on starch gel showed that the liver specific LDH-C4 isozymes of both the egg-mRNA and liver-mRNA injected goldfish resembled the control goldfish except an additional intermediate band. The migration distance of this new band matched the third band of the hybrids （carp× goldfish）. These findings lead to the conclusion that carp egg-mRNA contains a fraction partly responsible for liver development. Rat liver was found possessing the cathodally migrating C4 isozymes. Injection of rat liver-mRNA into goldfish eggs resulted in the intensification of the liver specific C4 isozymes. It appears therefore that the input informational molecules （liver-mRNA） and the output molecular products (liver C4 iszym

关键词： mRNA MEDIATED ALTERATION OF THE LIVER SPECIFIC ISOZYMES genetic manipulation in higher organisms GOLDFISH OVA AS MATERIALS OF OPERATION

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genetic manipulation in higher organisms Ⅲ.DETECTION OF SOYA PROTEIN IN SEEDS DERIVED FROM SOYA mRNA-TREATED RICE

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Science in China,Ser.A 1980年第1期 119-123页

作者：牛满江张葆英林忠平马诚张玉廉 Department of Biology Temple University Institute of Botany Academia Sinica Institute of Zoology

Poly A-attached RNA is isolated from soya seedlings. It is injected into ovaries offlowered rice and also used to treat anther. The RNA-treated anthers are cultured in *** are then induced from those anthers. Some of the developed calli give rise to *** from both series are found through immune reaction to contain soya protein while thecontrol has none.

关键词： mRNA genetic manipulation in higher organisms DETECTION OF SOYA PROTEIN IN SEEDS DERIVED FROM SOYA mRNA-TREATED RICE

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