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The g-protein β subunit AgB1 Promotes Hypocotyl Elongation through Inhibiting Transcription Activation Function of BBX21 in Arabidopsis

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Molecular Plant 2017年第9期10卷 1206-1223页

作者： Dong-bei Xu Institute of Crop Sciences Chinese Academy of Agricultural Sciences (CAAS)/National Key Facility for Crop Gene Resources and Genetic Improvement Key Laboratory of Biology and Genetic Improvement of Triticeae Crops Ministry of Agriculture Beijing 100081 China Institute of Botany Jiangsu Province and Chinese Academy of Sciences No.1 Qianhu Houcun Zhongshanmen Wai Nanjing Jiangsu Province 210014 PR China Beijing Engineering Research Center for Hybrid Wheat Beijing Academy of Agriculture and Forestry Sciences Beijing 100097 China College of Agronomy Northwest A&F University Yangling Shaanxi 712100 China

Hypocotyl development in Arabidopsis thaliana is regulated by light and endogenous hormonal cues, mak- ing it an ideal model to study the interplay between light and endogenous growth regulators. BBX21, a B-box （BBX）-Iike zinc-finger transcription factor, integrates light and abscisic acid signals to regulate hypocotyl elongation in Arabidopsis. Heterotrimeric g-proteins are pivotal regulators of plant development. The short hypocotyl phenotype of the g-protein I^-subunit （AgB1） mutant （agbl-2） has been previously identified, but the precise role of AgB1 in hypocotyl elongation remains enigmatic. Here, we show that AgB1 directly interacts with BBX21, and the short hypocotyl phenotype of agbl-2 is partially suppressed in agb1-2bbx21-1 double mutant. BBX21 functions in the downstream of AgB1 and overexpression of BBX21 in agbl-2 causes a more pronounced reduction in hypocotyl length, indicating that AgB1 plays an oppositional role in relation to BBX21 during hypocotyl development. Furthermore, we demonstrate that the C-terminal region of BBX21 is important for both its intracellular localization and its transcriptional activation activity that is inhibited by interaction with AgB1. ChiP assays showed that BBX21 specifically associates with its own promoter and with those of BBX22, HY5, and gA2oxl. which is not altered in agbl-2. These data suggest that the AgB1-BBX21 interaction only affects the transcrip- tional activation activity of BBX21 but has no effect on its DNA binding ability. Taken together, our data demonstrate that AgB1 positively promotes hypocotyl elongation through repressing BBX21 activity.

关键词： Arabidopsis BBX21 g-protein 13 subunit hypocotyl elongation transcriptional activation

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