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中国植物病理学会2024年学术年会

作者： ZENG Quan YU Quan MO Yingxi LIANG Haoming CHEN Baoshan MENG Jiaorong State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources and Ministry and Province co-sponsored Collaborative Innovation Center for Sugarcane and Sugar Industry Guangxi University College of Life Science and Technology Guangxi University Guangxi Key Laboratory of Sugarcane Biology and Academy of Sugarcane and Sugar Industry College of AgricultureGuangxi University

fusarium sacchari is one of the causal agents of Pokkah boeng,an important disease of sugarcane *** acyl-CoA dehydrogenases(ACADs) are a family of flavoenzymes involved in the β-oxidation of fatty acid and amino acid catabolism in ***,little is known about the role of the ACADs in the pathogenesis of ***,and there was no systemic report on the gene family *** prior study,a mutant library of *** was generated,and subsequent pathogenicity evaluations led to the discovery of a mutant harboring an insertion in the FsACAD gene,exhibiting diminished *** this study,a total of 14 ACAD-encoding genes(FsACAD-1 through FsACAD-14) were identified by screening the whole genome sequence of *** FsACAD genes were found to distribute across seven chromosomes and could be classified into six clades by phylogenetic analysis of the protein *** vivo mRNA quantification unveiled that FsACAD genes expressed differentially during infection of the *** expression patterns of FsACAD genes also differed significantly in response to the induction of different class of fatty ***,fatty acid utilization assays of the FsACAD-deletion mutants revealed that different ACADs varied in their preference and ability to breakdown the fatty acids with different carbon chain *** a result,different FsACAD-deletion mutants exhibited varied degree of adverse impact on the biological traits of the fungus,including growth,conidiation,tolerance to stress,and *** findings provide in insights into the comprehensive roles of the FsACADs in *** and may facilitate the future effort to identify key FsACAD targets for better control of Pokkah boeng disease.

关键词： fusarium sacchari Pokkah boeng disease ACAD fatty acid β-oxidation

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Microbial transformation of ginsenoside Rb1,Rb3 and Rc by fusarium sacchari

Microbial transformation of ginsenoside Rb1,Rb3 and Rc by Fu...

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2008 International Conference on Ginseng

作者： ZHAO Yuqing~1 HAN Ying~2 1 Department of Traditional Chinese Material Medica,Shenyang Pharmaceutical University,Shenyang,110016,P.R.China 2 College of Land and Environment,ShenyangAgricultural University,Shenyang,110161,P.R.China

Purpose Ginseng saponins(ginsenosides)are the principal components with anti-diabetic and anti-tumor *** 30 ginsenosides have already been isolated and identified from ginseng *** studies have shown that transformation of ginsenosides into deglycosylated ginsenosides is required for more effective physiological action of ginsenosides in *** paper report that ginsenosides Rb,Rb and Rc belonging to the major protopanaxadiol ginsenosides,were transformed using fusarium sacchari,a filamentous fungus firstly obtained and identified from the soil-cultivated ginseng with better transformation *** Metabolites were elucidated on the basis of physicochemical constants and NMR spectra and the kinetic curves for substrate and metabolites were determined by *** The transform parthway of Rb, Rb and Rc from ginsenoside by fusarium sacchari were as follows:Rb was transformed into compound K via Rd and F,Rb was transformed into compound Mx via gypenoside IX,Rc was transformed into compound K via Fe and *** The result suggest that fusarium sacchari was a fungus with strong transformation ability and the transformation of ginsenoside by F. sacchari were selectively hydrolyzed firstly at glycosidic bond of glucose and glucose,while the other type of glycosidic bond taking second place.

关键词： transformation ginsenoside Rb1 Rb3 and Rc fusarium sacchari

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甘蔗镰孢菌碳水化合物结合模块效应基因Fs11724的分离鉴定及功能分析

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热带作物学报 2022年第1期43卷 34-42页

作者：李慧雪黄振周宇明暴怡雪姚姿婷张木清姚伟亚热带农业生物资源保护与利用国家重点实验室广西南宁530004 广西甘蔗生物学重点实验室广西南宁530004 广西大学农学院广西南宁530004

甘蔗镰孢菌(fusarium sacchari)是引起毁灭性病害甘蔗梢腐病(sugarcane pokkah boeng disease, PBD)的主要病原菌之一。病原菌分泌的效应蛋白在病原与植物互作中发挥至关重要的作用。本研究基于课题组前期甘蔗镰孢菌全基因测序数据,设... 详细信息

甘蔗镰孢菌(fusarium sacchari)是引起毁灭性病害甘蔗梢腐病(sugarcane pokkah boeng disease, PBD)的主要病原菌之一。病原菌分泌的效应蛋白在病原与植物互作中发挥至关重要的作用。本研究基于课题组前期甘蔗镰孢菌全基因测序数据,设计基因特异性引物,通过RT-PCR扩增得到一个新的含CBM4;结构域的效应蛋白基因,并对其功能进行了初步探索。序列分析表明Fs11724基因开放阅读框822 bp,编码273个氨基酸,分子量为28.24 kDa,理论pI值为4.29,亲水性平均系数–0.072,属于亲水性分泌蛋白;通过农杆菌介导的烟草瞬时表达系统证实Fs11724抑制由BAX诱导的细胞程序性死亡,表明Fs11724具有潜在抑制寄主防御反应的毒性功能;同时,利用酵母分泌系统对其编码蛋白信号肽的分泌功能进行验证,确定该基因编码蛋白为典型分泌蛋白;qRT-PCR结果分析表明Fs11724在甘蔗镰孢菌侵染前、后期上调表达,表达量在168 hpi达到峰值。该研究表明Fs11724编码蛋白为甘蔗镰孢菌F. sacchari的一个效应蛋白,其信号肽具有分泌功能,并且可以在非寄主植物烟草上抑制BAX诱导的细胞坏死,在真菌侵染植物过程中显著表达,可能在调控植物免疫反应中发挥作用。研究结果为进一步研究病原–甘蔗互作,揭示甘蔗镰孢菌发病机制奠定基础。

关键词：甘蔗 fusarium sacchari CBM 效应蛋白 qRT-PCR

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Evaluation of Endophytic Bacteria for Plant Growth Promotion and Pathogen Suppression Traits in Saccharum officinarum

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Journal of Agricultural Science and Technology(A) 2017年第8期7卷 537-545页

作者： Penumatsa Kishore Varma Kotam Vijay Krishna Kumar Vanapalli Chandra Sekhar Dandu Adilakshmi Madugula Suresh Nambala Raja Kumar Karnam Jayaehandra Ravuri Anitha Department of Plant Pathology Regional Agricultural Research Station Anakapalle 531001 Andhra Pradesh lndia Department of Genetics and Plant Breeding Regional Agricultural Research Station Anakapalle 531001 Andhra Pradesh lndia

Endophytes are beneficial microbes that are capable of promoting growth, besides protecting colonized plants against plant pathogens. These microbes are of either bacterial, fungal or actinomycetes in plants. In the study, the endophytic bacteria isolated from sugarcane with their characterization related to plant growth promotion and pathogen suppression have been reported. Roots, shoots and leaves of rooted tissue culture plantlets of sugarcane cultivars of 87A298 and 2009A107 were excised aseptically and isolated endophytic bacterial strains. The strains were identified using 16S rRNA gene sequence based homology. Molecular characterization of these strains was carried out for presence of antimicrobial genes. The results showed that the endophytes isolated from sugarcane tissue culture plantlets were of the genera Bacillus （B. amyloliquefaciens, B. subtilis, B. cereus, B. safensis, B. siamensis, B. aryabhattai, B. flexus and B. velezensis） and Paenibacillus pabuli. There were three antimicrobial peptides （AMPs） producing genes of bacilysin, bacillomycin and fengycin in B. amyloliquefaciens （SE1, SE7）, B. siamensis （SE4, SE16）, B. subtilis （SE2, SE3） and B. velezensis （SE15）. The biochemical characterization assays showed that some of these strains could produce hydrogen cyanide （HCN）, protease, cellulase and indole acetic acid （IAA）. Few strains （SE1 and SE4） were phosphate solubilizers, whereas nine isolates were found to be diazotrophs. Most of the bacterial isolates were found antagonistic to fusarium sacchari, the sugarcane wilt pathogen under in vitro conditions. Overall, the results suggested the scope and potentiality of sugarcane endophytic bacteria, isolated from tissue culture plantlets, in promoting plant growth and suppression of sugarcane pathogen.

关键词： Bacillus fusarium sacchari Paenibacillus antimicrobial peptides.

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