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园艺学报 2023年第2期50卷 279-294页

作者：张欣漆艳香曾凡云王艳玮谢培兰谢艺贤彭军中国热带农业科学院环境与植物保护研究所农业农村部热带作物有害生物综合治理重点实验室海南省热带农业有害生物监测与控制重点实验室海口571101

香蕉枯萎病病原菌尖孢镰刀菌古巴专化型(fusarium oxysporum ***)4号生理小种(foc4)含有两个进化上高度保守的Dicer-like基因focDCL1和focDCL2。为了探究该基因的功能及其在RNAi中的作用机制,利用同源重组的方法获得ΔfocDCL1、ΔfocDCL... 详细信息

香蕉枯萎病病原菌尖孢镰刀菌古巴专化型(fusarium oxysporum ***)4号生理小种(foc4)含有两个进化上高度保守的Dicer-like基因focDCL1和focDCL2。为了探究该基因的功能及其在RNAi中的作用机制,利用同源重组的方法获得ΔfocDCL1、ΔfocDCL2和ΔfocDCL1/2基因敲除突变体。表型分析显示,与foc4野生型相比,突变体菌丝的营养生长无显著差异,但产孢量下降;ΔfocDCL2突变体在非生物胁迫刚果红处理后菌落明显变小且气生菌丝增多;ΔfocDCL2和ΔfocDCL1/2的致病力下降。miRNA深度测序数据显示,与foc4野生型相比,ΔfocDCL1、ΔfocDCL2和ΔfocDCL1/2敲除突变体的miRNA长度分布和5′–端首位碱基出现频率都发生了改变,都能产生自身特异miRNA。DCL功能存在交叉和冗余,miRNA可以通过依赖独立的、交叉的或者联合的DCL发生;此外,鉴定到不依赖DCL形成的miRNA。这些结果表明focDCL在产孢量、非生物胁迫、致病力以及小RNA发生中发挥作用。

关键词：香蕉枯萎病 fusarium oxysporum f.sp.cubense Dicer-like基因致病力测试 miRNA

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Studies on infection of fusarium oxysporum *** in banana by using green fluorescent protein reporter

Studies on infection of Fusarium oxysporum *** in banana by ...

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第三届全国微生物资源学术暨国家微生物资源平台运行服务研讨会

作者：肖荣凤刘波 fujian Academy of Agricultural Sciences

fusarium wilt of banana(Musa spp.) caused by fusarium oxysporum ***(foc) was one of the most serious fungal diseases in banana.A highly pathogenic strain fJAT-3076 of foc(race 4) was transformed with genes coding for Green fluorescent Protein(GfP),and used to follow colonization of root,rhizome and pseudostem tissues in Cavendish cultivar of *** 3-10 days after inoculation,the conidia and hyphae were observed to penetrate the young roots *** hyphae were found inside the root xylem by 10 *** symptoms progressed,the hyphae continued to upwards the rhizome and pseudostem xylem by 17 *** plants inoculated with foc died after 24 days,whilst foc had spread all over the xylem and part of hyphae reached the pseudostem ***,the highest level of hyphal density was observed in the pseudostem,while middle in root and least in *** it could effectively prevent the hyphae from the rhizome up to the pseudostem,the foc might be delayed or controlled.

关键词： banana infection fusarium oxysporum f.sp.cubense GfP

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Screening of antagonistic actinomyces against fusarium oxysporum *** race 4 and identification of strain Da07210

Screening of antagonistic actinomyces against Fusarium oxysp...

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2012年海南省微生物学会学术年会

作者： Jun Zhu Bo Chen Hui-qing Huang Min Liu Ye-hui Zheng Shi-xiang Bao Key Laboratory of Tropical Crop Biotechnology Ministry of Agriculture/Institute of Tropical Bioscience and BiotechnologyChinese Academy of Tropical Agricultural Sciences

Using fusarium oxysporum *** race 4 as target strain,antagonistic actinomycete strains were screened from the soil of Yingeling virgin forest and the healthy plants of diseased orchards in Hainan *** them strain Da07210 possessed prominent bioactivity and genetic *** the basis of morphology, physiological and biochemical properties,16S rDNA and phylogenetic tree,the strain was identified as Streptomyces noursei.

关键词： fusarium oxysporum f.sp.cubense antagonistic actinomyces screening identification

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The differential expressed genes of banana induced by fusarium oxysporum *** infection

The differential expressed genes of banana induced by Fusari...

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第十届全国植物结构与生殖生物学学术研讨会

作者： Tiecheng Peng Yunyun Chen Jingyao Luo Hua Tang National Key Laboratory Base of Tropic Biological Resources Sustainable Utilization College of Agriculture Hainan University

fusarium wilt of banana is a destructive fungus disease caused by fusarium oxysporum *** disease had harmful effects on banana planting and its industrial *** order to study the disease pathogenic mechanism of banana,we used cDNA-AfLP technique to identify transcripts that were induced or inhibited by fusarium oxysporum *** *** 222 isolated cDNA fragments,we sequenced and get 137 unique cDNAs that involved in disease and defense response,transcription regulation,signal transduction,primary metabolism,energy metabolism,cell growth and division,protein destination and storage,*** these ESTs,9 ESTs were related to disease and defense response,including NBS-LRR type resistance gene,hot shock protein,and alcohol dehydrogenase,ect;19 ESTs were related to transcriptional regulation,including the transcription factor MYB and MYC,zinc finger protein,retrotransposon,and Glycine-rich RNA binding protein,ect;13 ESTs were related to signal transduction,including the ubiquitin-conjugating enzyme E2,ect.6 important genes were chosen out to analysis the expression patterns through sqRT-PCR,and analyzed their relationship with pathogenic mechanism of banana fusarium wilt.

关键词： Banana fusarium oxysporum f.sp.cubense Differential expressed gene cDNA-AfLP Semi-quantitative RT-PCR

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Construction of PEG-mediated Genetic Transformation and Gene Knockout System in fusarium oxysporum f. *** Tropic Race 4

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Agricultural Biotechnology 2020年第1期9卷 15-17,21页

作者： Lei ZHANG Yan GUO Yunyue WANG Weihua TANG Sijun ZHENG State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan Ministry of Education Key Laboratory of Agriculture Biodiversity for Plant Disease ManagementYunnan Agricultural UniversityKunming 650201China Agricultural Environment and Resources Institute Yunnan Academy of Agricultural SciencesKunming 650205China Institute of Plant Physiology and Ecology Shanghai Institutes for Biological SciencesChinese Academy of SciencesShanghai 200032China Bioversity International Kunming 650205China

fusarium wilt of banana, caused by fusarium oxysporum f. sp. cubense tropic race 4(foc TR4), is a typical vascular and soil-borne disease which has significantly threatened the sustainable development of banana industry. In order to reveal the infection process and pathogenesis of foc TR4, the young mycelia(66.7 mg/ml) of wild-type strain of foc TR4(WT-foc TR4) cultured for 18-20 h were lysed with enzyme mixture for protoplast formation, which consisted of 25 mg/ml driselase, 0.4 mg/ml chitinase, 15 mg/ml lysing enzyme and 1.2 mol/L potassium chloride. The resulted protoplasts of 2×10~7 cells/ml were used to test the efficiency of transformation mediated by polyethylene glycol, and up to 9 transformants per microgram of DNA were obtained. AmCyan, RfP and YfP genes were stably transferred into the WT-foc TR4, separately, using the protoplast transformation system. The gene foOCH1 encoding α-1, 6-mannosyltransferase in the WT-foc TR4 was knocked out using the split-marker recombination technology. The genetic transformation and gene knockout system in this pathogen lays a foundation for the study of functional genomics and plant-pathogen interactions.

关键词： fusarium wilt of banana fusarium oxysporum f.sp.cubense Protoplasts Transformation split-marker recombination

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Optimization of fermentation conditions through response surface methodology to enhanced antibacterial metabolite production that Streptomyces sp.1-14 isolated from the Cassava rhizosphere soil

Optimization of fermentation conditions through response sur...

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中国植物病理学会第十一届全国会员代表大会暨2018年学术年会

作者：云天艳 feng Renjun Zhou Dengbo Pan Yueyun Chen Yufeng Wang fei Yin Liyan Zhang Yindong 谢江辉 Institute of Tropical Agriculture and forestry Hainan University Key Laboratory of Biology and Genetic Resources of Tropical Crops Ministry of AgricultureInstitute of Tropical Bioscience and BiotechnologyChinese Academy of Tropical Agricultural Sciences

Streptomyces sp.1-14 isolated from the rhizosphere soil of cassava were evaluated for antibacterial efficacy against fusarium oxysporum *** race 4(fOC4).Among 63 strains tested,thirteen recorded potent antibacterial properties and were further screened against eight bacterial pathogens.A strain showing maximum inhibition against all the test pathogens was identified by 16S rDNA sequencing as Streptomyces sp.1-14 and was selected for further *** propagation of Streptomyces 1-14 in soil under simulated conditions,can found that fOC4 had no significant influence on multiplication and survival of Streptomyces 1-14 in soil,but indigenous microorganisms in soil had significant influence on the counts of Streptomyces *** achieve maximum metabolite production was optimized through response surface methodology employing Plackett-Burman,the path of steepest ascent test and Box-Behnken *** composition of the final optimized fermentation conditions was glucose,38.877 g/L;CaCl·2HO,0.161 g/L;temperature,29.97℃;and inoculation amount,8.93%.The optimization resulted antibacterial activity of 56.13%against fOC4,which was 12.33%higher than that before optimization(43.80%).The results obtained that using response surface methodology to optimize the fermentation medium had a significant effect on the production of bioactive metabolites by Streptomyces *** the other hand,pH,light,storage temperature,*** fermentation must be closely monitored to reduce the formation of fermentation products with reduced antibacterial *** method is useful for further investigation of the production of anti-fOC4 substances,can be used to develop bio-control agents to prevent and control banana fusarium wilt.

关键词： Streptomyces iranensis 1-14 fusarium oxysporum f.sp.cubense Response surface methodology Bioactive metabolites

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