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Heat stress impacts the multi-domain ruminal microbiota and some of the functional features independent of its effect on feed intake in lactating dairy cows

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Journal of Animal Science and Biotechnology 2022年第6期13卷 1902-1916页

作者： Tansol Park Lu Ma Shengtao Gao Dengpan Bu Zhongtang Yu Department of Animal Sciences The Ohio State UniversityColumbusOHUSA Department of Animal Science and Technology Chung-Ang UniversityAnseong-siGyeonggi-doRepublic of Korea State Key Laboratory of Animal Nutrition Institute of Animal ScienceChinese Academy of Agricultural SciencesBeijing 100193People’s Republic of China CAAS-ICRAF Joint Lab on Agroforestry and Sustainable Animal Husbandry Beijing 100193People’s Republic of China

Background:Heat stress(HS)affects the ruminal microbiota and decreases the lactation performance of dairy *** HS decreases feed intake,the results of previous studies were confounded by the effect of HS on feed *** study examined the direct effect of HS on the ruminal microbiota using lactating Holstein cows that were pair-fed and housed in environmental chambers in a 2×2 crossover *** cows were pair-fed the same amount of identical total mixed ration to eliminate the effect of feed or feed *** composition and structure of the microbiota of prokaryotes,fungi,and protozoa were analyzed using metataxonomics and compared between two thermal conditions:pair-fed thermoneutrality(PFTN,thermal humidity index:65.5)and HS(87.2 for daytime and 81.8 for nighttime).Results:The HS conditions altered the structure of the prokaryotic microbiota and the protozoal microbiota,but not the fungal *** stress significantly increased the relative abundance of Bacteroidetes(primarily Gram-negative bacteria)while decreasing that of Firmicutes(primarily Gram-positive bacteria)and the Firmicutes-toBacteroidetes *** genera were exclusively found in the heat-stressed cows and thermal control *** co-occurrence and mutual exclusion between some genera were also found exclusively for each thermal *** stress did not significantly affect the overall functional features predicted using the 16S rRNA gene sequences and ITS1 sequences,but some enzyme-coding genes altered their relative abundance in response to ***:Overall,HS affected the prokaryotes,fungi,and protozoa of the ruminal microbiota in lactating Holstein cows to a different extent,but the effect on the structure of ruminal microbiota and functional profiles was limited when not confounded by the effect on feed ***,some genera and co-occurrence were exclusively found in the rumen of heat-stressed *** effects should be attributed to the direct effect

关键词： functional profiles Heat stress Microbiome Multi-kingdom Network analysis Ruminal microbiota

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Dietary energy sources and levels shift the multi-kingdom microbiota and functions in the rumen of lactating dairy cows

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Journal of Animal Science and Biotechnology 2020年第4期11卷 1073-1088页

作者： Tansol Park Lu Ma Ying Ma Xiaoqiao Zhou Dengpan Bu Zhongtang Yu Department of Animal Sciences The Ohio State UniversityColumbusOHUSA The State Key Laboratory of Animal Nutrition Institute of Animal ScienceChinese Academy of Agricultural SciencesBeijingP.R.China CAAS-ICRAF Joint Lab on Agroforestry and Sustainable Animal Husbandry BeijingP.R.China.

Background: Dietary energy source and level in lactation diets can profoundly affect milk yield and *** dietary effects on lactation performance are underpinned by alteration of the rumen microbiota, of which bacteria, archaea, fungi, and protozoa may vary differently. However, few studies have examined all the four groups of rumen microbes. This study investigated the effect of both the level and source of dietary energy on rumen bacteria, archaea, fungi, and protozoa in the rumen of lactating dairy cows. A 2 × 2 factorial design resulted in four dietary treatments: low and high dietary energy levels(LE: 1.52–1.53;and HE: 1.71–1.72 Mcal/kg dry matter) and two dietary energy sources(GC: finely ground corn;and SFC: steam-flaked corn). We used a replicated 4 × 4 Latin square design using eight primiparous Chinese Holstein cows with each period lasting for 21 d. The rumen microbiota was analyzed using metataxonomics based on kingdom-specific phylogenetic markers [16 S r RNA gene for bacteria and archaea, 18 S r RNA gene for protozoa, and internally transcribed spacer 1(ITS1) for fungi] followed with subsequent functional prediction using ***: The GC resulted in a higher prokaryotic(bacterial and archaeal) species richness and Faith's phylogenetic diversity than SFC. For the eukaryotic(fungi and protozoa) microbiota, the LE diets led to significantly higher values of the above measurements than the HE diets. Among the major classified taxa, 23 genera across all the kingdoms differed in relative abundance between the two dietary energy levels, while only six genera(none being protozoal)were differentially abundant between the two energy sources. Based on prokaryotic amplicon sequence variants(ASVs) from all the samples, overall functional profiles predicted using PICRUSt2 differed significantly between LE and HE but not between the two energy sources. Fish Taco analysis identified Ruminococcus and Coprococcus as the taxa potentially contributing

关键词： Corn processing method Dietary energy level functional profiles Multi-kingdom Rumen microbiota

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