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fucosylation is a common glycosylation type in pancreatic cancer stem cell-like phenotypes

World Journal of Gastroenterology 2015年第13期21卷 3876-3887页

作者： Naoko Terao Shinji Takamatsu Tomomi Minehira Tomoaki Sobajima Kotarosumitomo Nakayama Yoshihiro Kamada Eiji Miyoshi Department of Molecular Biochemistry and Clinical Investigation Osaka University Graduate School of MedicineSuita 565-0871Japan

AIM: To evaluate/isolate cancer stem cells(CSCs) from tissue or cell lines according to various definitions and cell surface markers. METHODS: Lectin microarray analysis was conducted on CSC-like fractions of the human pancreatic cancer cell line Panc1 by establishing anti-cancer drug-resistant cells. Changes in glycan structure of CSC-like cells were also investigated in sphere-forming cells as well as in CSC fractions obtained from overexpression of CD24 and CD44. RESULTS: Several types of fucosylation were increased under these conditions, and the expression of fucosylation regulatory genes such as fucosyltransferases, GDP-fucose synthetic enzymes, and GDP-fucose transporters were dramatically enhanced in CSC-like cells. These changes were significant in gemcitabineresistant cells and sphere cells of a human pancreatic cancer cell line, Panc1. However, downregulation of cellular fucosylation by knockdown of the GDP-fucose transporter did not alter gemcitabine resistance, indicating that increased cellular fucosylation is a result of CSC-like transformation. CONCLUSION: fucosylation might be a biomarker of CSC-like cells in pancreatic cancer.

关键词： Anti-cancer drug resistance Cancer stem cells fuco

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