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Obstructor,a frankliniella occidentalis protein,promotes transmission of tomato spotted wilt orthotospovirus

Insect Science 2023年第3期30卷 741-757页

作者： Xiaobin Zheng Yanran Wan Min Tao Jiangjiang Yuan Kun Zhang Jing Wang Youjun Zhang Pei Liang Qingjun Wu Department of Plant Protection Institute of Vegetables and FlowersChinese Academy of Agricultural SciencesBejingChina Department of Entomology China Agricultural UniversityBejingChina

Tomato spotted wilt orthotospovirus(TSWV)causes substantial economic losses to vegetables and other *** is mainly transmitted by thrips in a persistent and proliferative manner,and its most efficient vector is the western flower thrips,frankliniella occidentalis(Pergande).In moving from the thrips midgut to the salivary glands in preparation for transmission,the virions must overcome multiple *** several proteins that interact with TSWV in thrips have been characterized,we hypothesized that additional thrips proteins interact with TSWV and facilitate its *** the current study,67 F occidentalis proteins that interact with GN(a structural glycoprotein)were identified using a split-ubiquitin membrane-based yeast 2-hybrid(MbY2H)*** proteins,apolipoprotein-D(ApoD),orai-2-like(Orai),and obstructor-E-like isoform X2(Obst),were selected for further study based on their high abundance and interaction strength;their interactions with Gn were confirmed by MbY2H,yeastβ-galactosidase and luciferase complementation *** relative expressions of ApoD and Orai were significantly down-regulated but that of Obst was significantly up-regulated in viruliferous *** interfering with Obst in larval stage,the TSWV acquisition rate in 3 independent experiments was significantly decreased by 26%,40%,and 35%,*** addition,when Obst was silenced in adults,the virus titer was significantly decreased,and the TSWV transmission rate decreased from 66.7%to 31.9%using the leaf disk method and from 86.67%to 43.33%using the living plant ***,the TSWV acquisition and transmission rates were not affected by interference with the ApoD or Orai *** results indicate that Obst may play an important role in TSWV acquisition and transmission in frankliniella occidentalis.

关键词： frankliniella occidentalis obstructor tomato spotted wilt orthotospovirus transmission virus-vector interaction

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The mRNA Expression Profiles of Five Heat Shock Protein Genes from frankliniella occidentalis at Different Stages and Their Responses to Temperatures and Insecticides

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Journal of Integrative Agriculture 2014年第10期13卷 2196-2210页

作者： WANG Hai-hong Stuart R Reitz WANG Li-xia WANG Shuai-yu LI Xue LEI Zhong-ren State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural Sciences Malheur County Extension Oregon State University The General Station of Plant Protection in Shandong

The westem flower thrips, frankliniella occidental＆（Pergande） is a highly invasive pest that is able to exploit many crops across a wide range of environmental conditions. Five full-length cDNAs of heat shock protein （HSP） genes （Fo-HSP90, Fo-HSP70, Fo-HSP60, Fo-HSP40 and Fo-HSP28.9） were cloned from F. occidentalis, and their expression profiles were investigated under conditions of thermal stress and insecticide exposure, and at different stages during development, using real-time quantitative PCR. All five gene sequences showed high similarity to homologs in other species, indicating the conserved fimction of this gene family. HSP60 represents an informative phylogenetic marker at the ordinal taxonomic level within Insecta, but HSP90, which has two homologous copies in Hymenoptera, was not informative. The expression of Fo-HSPs under thermal stress suggests that Fo-HSP90, Fo-HSP70, and Fo-HSP28.9 are inducible by both cold and heat stress, Fo-HSP40 is only heat-inducible, and Fo-HSP60 is thermally insensitive. There were two patterns of cold induction of Fo-HSPs： one is from 0 to 4℃ and the other is around -8℃. All five Fo-HSPs genes were induced by exposure to sublethal concentrations of the insecticide avermectin. The expression of the five Fo-HSPs during different developmental stages suggests that they all play a role in development of F. occidentalis.

关键词： frankliniella occidentalis heat shock protein genes environmental stress development expression profile

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Field resistance to spinosad in western flower thrips frankliniella occidentalis(Thysanoptera:Thripidae)

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Journal of Integrative Agriculture 2016年第12期15卷 2803-2808页

作者： LI Dong-gang SHANG Xiao-yong Stuart Reitz Ralf Nauen LEI Zhong-ren Si Hyeock Lee GAO Yu-lin State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural Sciences Institute of Plant Protection Dezhou Academy of Agricultural Sciences Malheur County Extension Oregon State University Bayer Crop Science RD-SMRPest Control Biology Department of Agricultural Biotechnology Seoul National University

The western flower thrips, frankliniella occidentalis, is one of the most destructive sucking pests of vegetables, fruits and ornamental crops in China. Spinosad is one of the most commonly used insecticides to manage thrips. To assess the incidence of spinosad resistance in F. occidentalis field populations in eastern China, survival rates for 24 different populations were compared with those of a susceptible laboratory strain. All populations showed significantly higher resistance to spinosad compared with the control as determined by comparing median lethal concentrations. Two populations from Shouguang and Liaocheng in Shandong Province were classified as having moderate and high levels of resistance to spinosad with a mean resistance ratio of 17.0 and 89.2, respectively. Our research indicates a widespread reduction in spinosad efficacy for controlling F. occidentalis field populations, and that resistance management strategies should be implemented as soon as practicable, to reduce the potential of progressive resistance development and loss of efficacy.

关键词： insecticide resistance spinosad bioassay frankliniella occidentalis

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The diurnal flight activity and influential factors of frankliniella occidentalis in the greenhouse

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Insect Science 2010年第6期17卷 535-541页

作者： Xing-Hui Liang Zhong-Ren Lei Jin-Zeng Wen Ming-Liang Zhu State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant Protection Chinese Academy of Agricultural Sciences Beijing China

The daily flight activity of westem flower thrips, frankliniella occidentalis （Pergande）（Thysanoptera： Thripidae） was examined using color, yellow-light and chemical attractant traps in a cucumber greenhouse and under controlled laboratory conditions. In the greenhouse, flying thrips were most abundant between hours 08：00-10：00, declined at mid-day, and then slightly increased during hours 14：00-16：00; however they decreased to a very low level at 18：00. The use of light traps showed no thrip flight activity during the night. The total number of thrips that flew onto cards on rainy or cloudy days was higher than that on sunny days. We reconfirmed that traps treated with attractant attracted 4.0-9.4 times more thrip than the untreated traps. Under laboratory controlled conditions, thrip flight activity was highest under light intensity between 4 000 and 6 000 lux, air temperature of 28℃, and RH of 70%. In addition, starved thrips flew more readily than non-starved thrips.

关键词： attractant daily flight activity frankliniella occidentalis factors traps

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CopE and TLR6 RNAi-mediated tomato resistance to western flower thrips

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Journal of Integrative Agriculture 2023年第2期22卷 471-480页

作者： Jelli VENKATESH Sung Jin KIM Muhammad Irfan SIDDIQUE Ju Hyeon KIM Si Hyeock LEE Byoung-Cheorl KANG Department of Agriculture Forestry and BioresourcesResearch Institute of Agriculture and Life SciencesPlant Genomics Breeding InstituteCollege of Agriculture and Life SciencesSeoul National UniversitySeoul 08826Republic of Korea Department of Agricultural Biotechnology Seoul National UniversitySeoul 08826Republic of Korea Vegetable Research Division National Institute of Horticulture and Herbal ScienceRural Development AdministrationJeonju 55365Republic of Korea Research Institute of Agriculture and Life Sciences Seoul National UniversitySeoul 08826Republic of Korea

The western flower thrips(WFT;frankliniella occidentalis)is a mesophyll cell feeder that damages many *** of WFT is complex due to factors such as high fecundity,short reproduction time,ability to feed on a broad range of host plants,and broad pesticide *** challenges have driven research into developing alternative pest control approaches for *** study analyzed the feasibility of a biological control-based strategy to manage WFT using RNA interference(RNAi)-mediated silencing of WFT endogenous *** the delivery of RNAi,we developed transgenic tomato lines expressing double-stranded RNA(dsRNA)of coatomer protein subunit epsilon(CopE)and Toll-like receptor 6(TLR6)from *** genes are involved in critical biological processes of WFT,and their dsRNA can be lethal to these insects when ingested *** WFT that fed on the transgenic dsRNAexpressing tomato flower stalk showed increased mortality compared with insects that fed on wild-type *** addition,WFT that fed on TLR6 and CopE transgenic tomato RNAi lines showed reduced levels of endogenous CopE and TLR6 transcripts,suggesting that their mortality was likely due to RNAi-mediated silencing of these ***,our findings demonstrate that transgenic tomato plants expressing dsRNA of TLR6 and CopE can be lethal to ***,suggesting that these genes may be deployed to control insecticide-resistant WFT.

关键词： coatomer protein subunit epsilon(CopE) frankliniella occidentalis insect resistance RNA interference Toll-like receptor 6(TLR6) tomato transgenics

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Compatibility of early natural enemy introductions in commercial pepper and tomato greenhouses with repeated pesticide applications

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Insect Science 2020年第5期27卷 1111-1124页

作者： Beatriz Dader Ignacio Colomer Angeles Adan Pilar Medina Elisa Vinuela Unidad de Proteccion de Cultivos Escuela Tecnica Superior de Ingenieria AgronomicaAlimentaria y de Biosistemas(ETSIAAB)Universidad Polit^cnica de Madrid(UPM)MadridSpain Departamento de Ingenieria Rural Escuela Superior de IngenieriaUniversidad de AlmeriaAlmeriaSpain

Successful integrated pest management in protected crops implies an evalu-ation of the compatibility of pesticides and natural enemies(NE),as control strategies that only rely on one tactic can fail when pest populations exceed NE activity or pests become resistant to *** in Almeria(Spain),growers release NE prior to transplanting or early in the crop cycle to favor their settlement before pest arrival because this improves biocontrol efficacy,although it extends pesticide exposure *** pur-pose of this research was to evaluate the compatibility of two applications of pesticides with key NE in 2-year trials inside tomato and sweet pepper commercial greenhouses:Nesidiocoris tenuis(Reuter)(Hemiptera:Miridae),Orius laevigatus(Say)(Hemiptera:Anthocoridae)and Amblyseius swirskii(Athias-Henriot)(Acari:Phytoseidac).In tomato,flubendiamide and chlorantraniliprole(IOBC category 1)were compatible with ***,but chlorpyrifos.:methyl and spinosad(IOBC categories 2-3),which efectively reduced Tiuta absoluta(Meyrick)(Lepidoptera:Gelechidae)density,compromised its predatory *** sweet pepper,chlorantraniliprole(IOBC category 1)was the only pesticide compatible with *** while chlorantraniliprole,emamectin benzoate,spirote-tramat and pymetrozine were harmless(IOBC category 1)to Amblyseius swirskii,and sulfoxaflor slightly harmful(IOBC category 2)to this phytoseiid predator.

关键词： Amblyseius swirskii chemical control frankliniella occidentalis Nesidiocoris tenuis Orius laevigatus Tuta absoluta

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Effects of single and dual species herbivory on the behavioral responses of three thrips species to cotton seedlings

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Insect Science 2017年第4期24卷 684-698页

作者： Rehan Silva Gimme H. Walter Lewis J. Wilson Michael J. Furlong School of Biological Sciences The University of Queensland St. Lucia Queensland 4072 Australia Cotton Research Unit CSIRO Agriculture Flagship Locked Bag 59 Narrabri NSW 2390 Australia

This study investigated the olfactory responses of 3 thrips species [frankliniella schultzei Trybom, E occidentalis Pergrande and Thrips tabaci Lindeman （Thysanoptera： Thripidae）] to cotton seedlings [Gossypium hirsutum L. （Malvales： Malvaceae）] simulta- neously damaged by different combinations of herbivores. Cotton seedlings were damaged by foliar feeding Tetranychus urticae Koch （Trombidiforms： Tetranychidae）, Helicoverpa armigera Hibner （Lepidoptera： Noctuidae）, Aphis＇ gossypii Glover （Hemiptera： Aphidi- dae） or root feeding Tenebrio molitor L. （Coleoptera： Tenebrionidae）. Thrips responses to plants simultaneously damaged by 2 species of herbivore were additive and equivalent to the sum of the responses of thrips to plants damaged by single herbivore species feeding alone. For example, E occidentalis was attracted to T. urticae damaged plants but more attracted to undamaged plants than to plants damaged by H. armigera. Plants simultane- ously damaged by low densities of T. urticae and H. armigera repelled E occidentalis but as T. urticae density increased relative to H. armigera density, E occidentalis attraction to coinfested plants increased proportionally. Thrips tabaci did not discriminate between un- damaged plants and plants damaged by H. armigera but were attracted to plants damaged by T. urticae alone or simultaneously damaged by T. urticae and H. armigera. Olfactometer assays showed that simultaneous feeding by 2 herbivores on a plant can affect predator- prey interactions. Attraction of F. occidentalis to plants damaged by its T. urticae prey was reduced when the plant was simultaneously damaged by H. armigera, T. molitor, or A. gossypii and F. schultzei was more attracted to plants simultaneously damaged by T. urticae and H. armigera than to plants damaged by T. urticae alone. We conclude that plant responses to feeding by I species of herbivore are affected by responses to feeding by other herbivores. These plant-mediated interactions between herbivo

关键词： attraction dual-herbivory frankliniella occidentalis frankliniella schultzei induced responses predictability simultaneous damage Thrips tabaci

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Occurrence Regularity and Control of Thrips on Leek

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Plant Diseases and Pests 2018年第3期9卷 33-39页

作者： Chen Dexi He Zhongquan Guo Yunjian Huang Tengfei Yi Xueqing Liu Huan Lu Daijuan Liu Fuping Institute of Plant Protection Sichuan Academy of Agricultural Sciences Chengdu 610066 China Agriculture and Forestry Bureau of Pidu District Pidu 610017 China

[Objective] The paper was to clear the species and occurrence regularity of thrips severely occurred in leek field, and to study the main control methods. [Methods] The species of thrips on leek were identified in Sichuan Province, and the damage symptoms, occurrence regularity and control methods of thrips in leek field were studied. [Result] The morphological characteristic of thrips on leek was frankliniella occidentalis(Pergande). ITS molecular identification results showed that the amplified fragment was 520 bp, which shared the similarity of 99.81% with the base se-quence of F. occidentalis. F. occidentalis concentrated at the height of 80 cm from the ground; in sunny days, the occurrence frequency of F. occidentalis was relatively high during 15:00 and 17:00; rainwater had obvious lethal effect on F. occidentalis, and the lethal rate was 29.30%. Field trial showed that 20% dinotefuran SG and 40% acetamiprid GR had good control effect against F. occidentalis. The control effect of 20% dinotefuran SG 1 500 times dilution was the best, up to 92.56%. [Conclusion] The results will provide effective technical support for large-scale control of thrips in leek field.

关键词： Leek frankliniella occidentalis Biological characteristics Occurrence regularity Control method

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